The use of Fourier transform infrared spectroscopy to assay for urease from Pseudomonas aeruginosa and Canavalia ensiformis

Karmali, K.; Karmali, Amin; Teixeira, Adriano; Marcelo‐Curto, M. J.

doi:10.1016/s0003-2697(04)00360-4

Cited by 17 publications

(8 citation statements)

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“…The reaction data recorded at 2 min intervals (Figure C) revealed that the formation of bicarbonate at 1361 cm –1 (υ a CO 2 ) was the most suitable method to determine the kinetics of urease by ATR-FTIR. These results are in accordance with those in the literature . For this reason, only the bicarbonate calibration curve is presented.…”

Section: Resultssupporting

confidence: 92%

“…These results are in accordance with those in the literature. 22 For this reason, only the bicarbonate calibration curve is presented. The calibration curve for bicarbonate at 1361 cm −1 (Figure 4D) was used to determine the urease activity by the concentration of the bicarbonate produced during urea hydrolysis.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In addition, many conventional methods have fallen into disuse due to several factors, such as delayed result acquisition, high cost of the reagents used in the analyses, and their toxicity. 22 Furthermore, the traditional methods adopting ultraviolet−visible spectroscopy cannot be used for the determination of urease enzyme kinetics in the presence of polyphenols because false-positive results can be observed because of their coloration and the immense presence of phenolic groups. 23,24 In this context, to improve our knowledge, this was the first study with polyphenols obtained from the bark of trees with a goal of agricultural application.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The monitoring of enzymatic kinetics using Fourier transform infrared spectroscopy (FTIR) with the attenuated total reflection (ATR) accessory can be determined either by substrate consumption or by product formation since both will present different spectra. , This method has considerable advantages over traditional methods since the enzymatic reaction can be studied in real time. In addition, many conventional methods have fallen into disuse due to several factors, such as delayed result acquisition, high cost of the reagents used in the analyses, and their toxicity . Furthermore, the traditional methods adopting ultraviolet–visible spectroscopy cannot be used for the determination of urease enzyme kinetics in the presence of polyphenols because false-positive results can be observed because of their coloration and the immense presence of phenolic groups. , …”

Section: Introductionmentioning

confidence: 99%

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Valorization of Polyphenols from Stryphnodendron adstringens Bark for Use as a Sustainable Inhibitor of Nitrogen Volatilization in Soil

Lorenço

Zidanes

Araújo

et al. 2021

ACS Agric. Sci. Technol.

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Urease (EC 3.5.1.5) activity is one of the processes that most contributes to urea loss by ammonia volatilization in agriculture. The present study describes the capacity of polyphenols extracted from barbatimão tree bark (Stryphnodendron adstringens (Mart.) Coville) on inhibiting the urease enzyme extracted from Canavalia ensiformis (L.) DC. Fourier transform infrared spectroscopy (FTIR) coupled to an attenuated total reflection (ATR) accessory was chosen to determine the enzymatic kinetics. This spectroscopic technique is used to perform more sustainable enzyme kinetic studies due to the decreased use of chemicals, including highly toxic reagents. The inhibition capacity of the polyphenols was compared with N-(n-butyl)thiophosphoric triamide (NBPT). The reactions occurred in 20 mM potassium phosphate buffer, pH 7.2, and the crude urease extract was used. The maximum intensity of asymmetric stretching of the bicarbonate functional group at 1361 cm–1 was measured to study the kinetics of urease. The kinetic constants V max and K m were determined by nonlinear fitting with the Michaelis–Menten model. The data obtained showed the strong potential of polyphenols from bark to be used as urease inhibitors on fertilizers in soil, decreasing the use of chemical reagents exogenous to the environment, such as NBPT.

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Section: Resultssupporting

confidence: 92%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Valorization of Polyphenols from Stryphnodendron adstringens Bark for Use as a Sustainable Inhibitor of Nitrogen Volatilization in Soil

Lorenço

Zidanes

Araújo

et al. 2021

ACS Agric. Sci. Technol.

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“…If water is suspected, thus obscuring the 1600 cm –1 region, bicarbonate may be detected by its 1360 cm –1 band, if enough resolution to discriminate against the 1300–1200 cm –1 cyanate modes is available. Even if urea were present, the 1360 cm –1 band would still be a good marker, as beautifully illustrated in Figure 1 of ref for biochemical media. If there is no water in the source under observation, then the best candidate would be the strong 1660 cm –1 mode, which would still be discernible in cyanate containing lines of sight.…”

Section: Conclusion and Astrophysical Implicationsmentioning

confidence: 96%

The Structure and Spectroscopy of Cyanate and Bicarbonate Ions. Astrophysical Implications

et al. 2013

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Cyanate and bicarbonate are two ions that play active roles in many fields of physics and chemistry, including biological sciences and astrochemistry. We present here a comprehensive study of these species covering a range of phases and methodologies. We have performed theoretical calculations on the isolated ions and their hydrates with one to four water molecules, and in clusters with 15 water molecules. The predicted infrared spectra are compared with observed spectra from experiments where liquid droplets of their solutions are frozen at 14 K on a substrate, to mimic some astrophysical conditions. Crystals of cyanate and bicarbonate sodium and potassium salts are also studied experimental and theoretically. As well, the spontaneous decomposition of cyanate into bicarbonate is documented from the spectra of an aged solution. Finally, the possible astrophysical observation of bicarbonate in watercontaining particles is discussed.

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Biosynthesis of gold nanoparticles using leaf extract of Salvadora persica and its role in boosting urease performance via immobilization

Singh

Kumari

Srivastava

et al. 2021

J. Plant Biochem. Biotechnol.

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The use of Fourier transform infrared spectroscopy to assay for urease from Pseudomonas aeruginosa and Canavalia ensiformis

Cited by 17 publications

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Valorization of Polyphenols from Stryphnodendron adstringens Bark for Use as a Sustainable Inhibitor of Nitrogen Volatilization in Soil

Valorization of Polyphenols from Stryphnodendron adstringens Bark for Use as a Sustainable Inhibitor of Nitrogen Volatilization in Soil

The Structure and Spectroscopy of Cyanate and Bicarbonate Ions. Astrophysical Implications

Biosynthesis of gold nanoparticles using leaf extract of Salvadora persica and its role in boosting urease performance via immobilization

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