The use of enzymes in organic synthesis and the life sciences: perspectives from the Swiss Industrial Biocatalysis Consortium (SIBC)

Meyer, Hans‐Peter; Eichhorn, Eric; Hanlon, Steven P.; Lütz, Stephan; Schürmann, Martin; Wohlgemuth, Roland; Coppolecchia, Raffaella

doi:10.1039/c2cy20350b

Cited by 100 publications

(63 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the conventional activation conditions, with succinic anhydride, the lipase PCL provides the best conversions (13 % \ C \ 80 %) and selectivities (1 \ E \ 46) for the studied substrates [1][2][3][4][5][6][7]. The best selectivity was observed for a-tetralol 1, E = 25 and C = 34 % (entry 2).…”

Section: Resultsmentioning

confidence: 80%

“…In recent years, biocatalyzed kinetic resolution associated with chemo-enzymatic methodologies are tools frequently used to prepare enantiomerically active molecules; its potential in organic catalysis is far from being fully exploited [1][2][3][4]. Very high enantio-, regio-and chemo-selectivities can be achieved using hydrolases due to the strict recognition of the substrate by the enzyme [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Easy preparation of enantiomerically enriched heteroaromatic alcohols through lipase-catalyzed acylation with succinic anhydride under unconventional activation

Melais

Boukachabia

Aribi‐Zouioueche

et al. 2015

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

This study reports the lipase-catalyzed resolution of heteroaromatic secondary alcohols by succinic anhydride under different activation conditions by convenient procedure with succinic anhydride. The effects of succinic anhydride and the nature of the heteroatom are discussed in standard conditions in the kinetic resolution with lipases. The results recorded under microwave activation and ultrasonication is compared. (R)-4-chromanol was obtained in optically pure form (ee > 99%) with a high selectivity E > 200 by Pseudomonas cepacia lipase (PCL) in diethyl ether, using microwave radiation and under ultrasonication. The reaction time is reduced compared to the conventional heating with a better control of the selectivity of the lipase PCL. A significant effect of the nature of the heteroatoms on the reactivity and selectivity of the lipase with succinic anhydride has been disclosed, regardless the conditions of activation. This method proved to be clean, fast, interesting alternative, and facilitates the use of a cyclic anhydride, by microwave or ultrasound especially with secondary alcohols. The process is a valuable prerequisite for the preparative scale production of enantiomerically heteroaromatic alcohols in sustainable chemistry.

show abstract

Section: Resultsmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Easy preparation of enantiomerically enriched heteroaromatic alcohols through lipase-catalyzed acylation with succinic anhydride under unconventional activation

Melais

Boukachabia

Aribi‐Zouioueche

et al. 2015

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

show abstract

“…Diverse examples of chiral medicaments are produced by biocatalyzed process, which include atorvastatin (Lipitor), rosuvastatin (Crestor), sitagliptin (Januvia), and montelukast (Singulair). Industrial biocatalysis are still an exception and not a rule, although the use of enzymes has been gaining increasing attention for the organic synthesis of high added-value products, such as pharmaceuticals, flavors and fragrances, vitamins, and fine chemicals as well as for some commodities [156,227]. Companies such as Avecia, Basf, Evonik, DSM, Dow Pharma, and Lonza use enzymes or cells for chiral synthesis of their products [228].…”

Section: Pharmaceutical Products and Chemicalsmentioning

confidence: 99%

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

et al. 2017

View full text Add to dashboard Cite

Abstract:The establishment of a bioeconomy era requires not only a change of production pattern, but also a deep modernization of the production processes through the implementation of novel methodologies in current industrial units, where waste materials and byproducts can be utilized as starting materials in the production of commodities such as biofuels and other high added value chemicals. The utilization of renewable raw resources and residues from the agro-industries, and their exploitation through various uses and applications through technologies, particularly solid-state fermentation (SSF), are the main focus of this review. The advocacy for biocatalysis in green chemistry and the environmental benefits of bioproduction are very clear, although this kind of industrial process is still an exception and not the rule. Potential and industrial products, such as biocatalysts, animal feed, fermentation medium, biofuels (biodiesel, lignocelulose ethanol, CH 4 , and H 2 ), pharmaceuticals and chemicals are dealt with in this paper. The focus is the utilization of renewable resources and the important role of enzymatic process to support a sustainable green chemical industry.

show abstract

“…The lack of enzyme stability of mesophilic enzymes renders them inefficient for the harsh reaction conditions required in industrial processes. For this reason, the use of biocatalysts in organic reactions represents only a small fraction of the potential industrial market (Meyer et al, 2012). Thermotolerant bacteria have attracted industrial and biotechnological attention as their enzymes are well suited for harsh industrial processes (Abdel-Rahman et al, 2016;Archna et al, 2015;Verma et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Thermotolerant bacteria of biotechnological potential from hot springs in Eritrea

Ghilamicael¹,

Budambula²,

Anami³

et al. 2018

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Thermophiles are excellent sources of enzymes that can withstand and carry out reactions efficiently under high temperatures. This study isolated and characterised thermotolerant bacteria that produce enzymes of potential industrial value from five hot springs in Eritrea. A total of 65 bacterial isolates were obtained from the five hot springs. Out of the 65 isolates; 19 isolates produced a positive reaction for amylases, 36 for carboxymethyl cellulases, eight for proteases, 10 for xylanases and 11 for pectinases. More than half (36 out of 65) were able to produce carboxymethyl cellulases. Six isolates which showed carboxymethyl cellulase activity were from the genus Bacillus, while those belonging to Brevibacillus were seven. BLAST analysis of the partial sequences showed that 19 out of the 24 isolates sequenced showed high similarity (> 99%) to those of reference strains of the genera Bacillus and Brevibacillus available in the Genebank and EZ-taxon databases. The five isolates (E5, G2, G8, M1 and M13) that showed moderate similarities (97.2-99%) to strains from the Genebank and EZ-taxon databases were further characterized. Physiological characterization of the five selected isolates based on tolerance to NaCl, temperature and production of hydrolytic enzymes indicated that these isolates are potentially novel. Isolates G8 and M13 showed significantly higher amylase activity (p < 0.05) than the other three isolates. Caseinase activity recorded by the five isolates was the highest (p < 0.05) compared to other enzyme activities. The enzymes produced by thermotolerant bacteria from the five hot springs may be potentially useful for catalysis under harsh operational conditions encountered in industrial processes.

show abstract

The use of enzymes in organic synthesis and the life sciences: perspectives from the Swiss Industrial Biocatalysis Consortium (SIBC)

Cited by 100 publications

References 33 publications

Easy preparation of enantiomerically enriched heteroaromatic alcohols through lipase-catalyzed acylation with succinic anhydride under unconventional activation

Easy preparation of enantiomerically enriched heteroaromatic alcohols through lipase-catalyzed acylation with succinic anhydride under unconventional activation

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

Thermotolerant bacteria of biotechnological potential from hot springs in Eritrea

Contact Info

Product

Resources

About