The Use of Dried Blood Spots for the Quantification of Antihypertensive Drugs

Chernonosov, Alexander А.

doi:10.1155/2018/3235072

Cited by 5 publications

(7 citation statements)

References 90 publications

(153 reference statements)

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“…The use of dried blood spots (DBSs) can simplify the methods for determining the concentrations of drugs [2]. The DBS sampling technique is suitable even for small children [5] for routine clinical testing [4].…”

Section: Fig 1 Chemical Structure Of Atenololmentioning

confidence: 99%

“…The DBS sampling technique is suitable even for small children [5] for routine clinical testing [4]. DBS helps with recruitment of subjects for preclinical or clinical studies [6] due to simpler storage and easier transfer by mail to the assigned analytical laboratory [2]. Chernonosov and Koval;JPRI,31(6): [1][2][3][4][5][6][7][8]2019; Article no.…”

Section: Fig 1 Chemical Structure Of Atenololmentioning

confidence: 99%

“…Using the DPS can solve this problem. In several works the DBS assay was used for atenolol determination [2], but there are no studies for atenolol quantification in DPS. Moreover only in one recent research authors discuss the atenolol extraction from DBS [16].…”

Section: Equipment and Hplc Conditionsmentioning

confidence: 99%

“…The main attributable risk factor of death is hypertension. The hypertension is also responsible for 50% of the cases of ischemic and cerebrovascular heart diseases [2,3]. For hypertension treatment various classes of antihypertensive drugs have been developed: diuretics, calcium channel blockers, blockers, blockers, etc.…”

Section: Introductionmentioning

confidence: 99%

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Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

Chernonosov

Koval

2019

JPRI

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Aims: Atenolol is one of the β-blockers widely used for the treatment of hypertension and other cardiovascular diseases. To simplify the methods for determining of drugs concentrations in blood and plasma the dried spots assays (dried blood spots or dried plasma spots) could be used. In this case high sensitive detector like mass-spectrometer is required as well as high level of drug recovery from dried spot. In this study the extraction of atenolol from dried plasma spots (DPS) was studied to offer the optimum parameters of extraction method. Study Design: Short research articles. Place and Duration of Study: Core Facility of Mass Spectrometric Analysis, Institute of Chemical Biology and Fundamental Medicine SB RAS, between January and October 2019. Methodology: The organic extraction method was chosen for evaluation as the most suitable for LC-MS assay. Several parameters: % of organic solvent, presence or absence of 0.1% formic acid, Short Research Articletime, volume and temperature of extraction were investigated to find the best combination for atenolol recovery from DPS for further LC Results: Results showed that the solvent composition and temperature has main influence on the extraction. The effect of extraction time and volume of solvent have no significant influence on atenolol recovery. Pure acetonitrile is the worst solvent for atenolol extraction from DPS. The solvents: MeOH:H 2 O (60:40, v:v), MeOH:0.1% FA in H v:v) or ACN:MeOH (60:40, v:v) provide the best recovery of atenolol temperature is 40°C, time of extraction is 15 Conclusion: Several solvents acceptable for LC from DPS can be used for routine extraction of atenolol.

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Section: Fig 1 Chemical Structure Of Atenololmentioning

confidence: 99%

Section: Fig 1 Chemical Structure Of Atenololmentioning

confidence: 99%

Section: Equipment and Hplc Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

Chernonosov

Koval

2019

JPRI

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“…DBS along with LC-MS/MS is now broadly applied for the quantitation of a wide variety of analytes from various therapeutic areas majorly in therapeutic drug monitoring and pharmacokinetic/toxicokinetics studies. Recent reviews on DBS methodology [4][5][6][7][8][9] is a true testimony of its utility in bioanalytical quantitation.…”

Section: Introductionmentioning

confidence: 99%

Quantitation of Ivosidenib, A Novel Mutant IDH1 Inhibitoron Mice DBS: Application to a Pharmacokinetic Study

2019

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Ivosidenib is an approved drug for relapsed or refractory IDH1 mutant AML patients. The goal of the present work is to develop and validate an LC-MS/MS method for the quantitation of ivosidenib in mice dried blood spots (DBS) as per regulatory guideline in the linearity range of 1.10–3293 ng/mL. To date there is no bioanalytical method reported for quantitation of ivosidenib. The chromatographic resolution of ivosidenib and internal standard (warfarin) was achieved on a C18 column with an isocratic mobile phase. All validation parameters met the acceptance criteria. The intra- and inter-day precision was in the range of 2.79–10.5 and 5.76–9.02%, respectively. Ivosidenib was stable for 3 freeze/thaw cycles, up to 7 days at room temperature and for one month at −80°C. The applicability of the validated method is shown in a mice pharmacokinetic study. Ivosidenib was quantifiable up to 24 and 36 h following intravenous and oral administration to mice, respectively. The oral bioavailability was 48%. Comparison of DBS vs. plasma concentrations of ivosidenib showed excellent correlation, indicating DBS can be used as an alternative for plasma for pharmacokinetic analysis.

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Closing the gap — development of an analytical methodology using volumetric absorptive microsampling of finger prick blood followed by LC-HRMS/MS for adherence monitoring of antihypertensive drugs

et al. 2022

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Volumetric absorptive microsampling (VAMS), an emerging microsampling technique, is a promising tool for adherence monitoring. This study focused on development of an analytical methodology to improve VAMS-based strategies for adherence assessment by analyzing angiotensin-converting-enzyme (ACE) inhibitors, loop diuretics, a potassium-sparing diuretic, and a thiazide diuretic. Development included sample preparation, chromatographic conditions, mass spectrometry settings, validation, and demonstrating proof of concept. Quantification of analytes, by name furosemide, hydrochlorothiazide, lisinopril, torasemide, and the active metabolites, canrenone, enalaprilat, and ramiprilat in finger prick blood (FPB), was validated based on international guidelines. Selectivity, carryover, and within/between-run accuracy and precision were in accordance with the recommendations. The matrix effect was evaluated at three different hematocrit levels (HT: 20%, 40%, 60%) and the coefficients of variation did not exceed 15%. Dilution integrity (1:10 and 1:20) was given for all analytes except lisinopril, yet for lisinopril, the therapeutic range was already covered by the calibration range. Long-term stability in VAMS tips was tested for 2 weeks at 24 °C in the dark and revealed no degradation of analytes. The proof of concept was performed by analyzing 35 intakes of ACE-inhibitors and diuretics in 18 VAMS and matched plasma samples. Hereby, determined concentration in FPB and plasma cannot be used interchangeably, and thus specific reference ranges for whole blood must be established. Nevertheless, the VAMS-based strategy was shown to be suitable for assessing adherence of all classes of antihypertensive drugs used in the guidelines to manage hypertension. Graphical abstract

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The Use of Dried Blood Spots for the Quantification of Antihypertensive Drugs

Cited by 5 publications

References 90 publications

Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

Quantitation of Ivosidenib, A Novel Mutant IDH1 Inhibitoron Mice DBS: Application to a Pharmacokinetic Study

Closing the gap — development of an analytical methodology using volumetric absorptive microsampling of finger prick blood followed by LC-HRMS/MS for adherence monitoring of antihypertensive drugs

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