Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

Abstract

Aims: Atenolol is one of the β-blockers widely used for the treatment of hypertension and other cardiovascular diseases. To simplify the methods for determining of drugs concentrations in blood and plasma the dried spots assays (dried blood spots or dried plasma spots) could be used. In this case high sensitive detector like mass-spectrometer is required as well as high level of drug recovery from dried spot. In this study the extraction of atenolol from dried plasma spots (DPS) was studied to offer the optimum parameters of extraction method. Study Design: Short research articles. Place and Duration of Study: Core Facility of Mass Spectrometric Analysis, Institute of Chemical Biology and Fundamental Medicine SB RAS, between January and October 2019. Methodology: The organic extraction method was chosen for evaluation as the most suitable for LC-MS assay. Several parameters: % of organic solvent, presence or absence of 0.1% formic acid, Short Research Articletime, volume and temperature of extraction were investigated to find the best combination for atenolol recovery from DPS for further LC Results: Results showed that the solvent composition and temperature has main influence on the extraction. The effect of extraction time and volume of solvent have no significant influence on atenolol recovery. Pure acetonitrile is the worst solvent for atenolol extraction from DPS. The solvents: MeOH:H 2 O (60:40, v:v), MeOH:0.1% FA in H v:v) or ACN:MeOH (60:40, v:v) provide the best recovery of atenolol temperature is 40°C, time of extraction is 15 Conclusion: Several solvents acceptable for LC from DPS can be used for routine extraction of atenolol.

Parallel Reaction Monitoring Mode for Atenolol Quantification in Dried Plasma Spots by Liquid Chromatography Coupled with High-Resolution Mass Spectrometry

“…We previously conducted experiments to optimize the parameters of atenolol extraction from DPS [28] by the MRM method. In this study, we utilized these optimized extraction parameters to develop and validate a quantitative assay of atenolol by HRMS.…”

“…In brief, extraction was carried out by means of 100 µL of a MeOH:H 2 O mixture (60:40, v/v) containing the IS for 15 min at 40 • C in a shaker at 900 rpm. The only change was a reduction of extraction volume from 200 µL [28] to 100 µL for concentrating the samples with a small amount of analyte.…”

Optimization of the Extraction Procedure of Apixaban from Dried Rat Plasma Spots

“…Currently, dried blood spots (DBS) technic is widely used in screening analysis, as well as for routine clinical research [6,10,11]. This method has various advantages, such as minimal invasiveness, minimal risk of infection with infectious pathogens, and ease of storage and transportation.…”