Parallel Reaction Monitoring Mode for Atenolol Quantification in Dried Plasma Spots by Liquid Chromatography Coupled with High-Resolution Mass Spectrometry

Abstract

In this study, we reported a rapid, sensitive, robust, and validated method for atenolol quantification in dried plasma spots (DPS) by liquid chromatography with high-resolution mass spectrometry (LC-HRMS) using parallel reaction monitoring mode (PRM). Aliquots of 25 µL human plasma were placed onto Whatman 903 Cards and air-dried. Disks (3.2 mm internal diameter) were punched, and a 100 µL working internal standard solution was added to each sample and then incubated on a shaker for 15 min at 40 °C, followed by rapid centrifugation (10,000× g, 10 s). The supernatant was transferred into 300 µL vials for subsequent LC–HRMS analysis. After chromatographic separation, atenolol and the internal standard were quantified in positive-ion parallel reaction monitoring mode by detection of all target product ions at 10 ppm tolerances. The total time of the analysis was 5 min. The calibration curve was linear in the range of 5–1000 ng/mL with interday and intraday precision levels and biases of <14.4%, and recovery was 62.9–81.0%. The atenolol in DPS was stable for ≥30 days at 25 and 4 °C. This fully validated method is selective and suitable for atenolol quantitation in DPS using LC–HRMS.

Stochastic Dynamic Mass Spectrometric Quantitative and Structural Analyses of Pharmaceutics and Biocides in Biota and Sewage Sludge

“…The peak at m / z 116 has been used to determine PRO in the liver, brain, and kidney thin tissue, as well [ 85 , 94 ]. In addition to the peak at m / z 116, ATE exhibits a set of ions depending on the experimental conditions [ 96 , 97 , 98 , 99 , 100 ]. These are peaks at m / z 225, 208, 190, 173, 162, and 145 [ 84 , 96 , 97 , 98 , 99 , 100 ].…”

“…In addition to the peak at m / z 116, ATE exhibits a set of ions depending on the experimental conditions [ 96 , 97 , 98 , 99 , 100 ]. These are peaks at m / z 225, 208, 190, 173, 162, and 145 [ 84 , 96 , 97 , 98 , 99 , 100 ]. Moreover, there are peaks at m / z 133, 115, and 107 [ 94 ] ( Figure S19 ).…”