The use of different glucose oxidases for the development of an amperometric reagentless glucose biosensor based on gold nanoparticles covered by polypyrrole

German, Natalija; Kausaitė-Minkstimienė, Asta; Ramanavičius, Arūnas; Semashko, T. V.; Михайлова, Р. В.

doi:10.1016/j.electacta.2015.04.072

Cited by 64 publications

(57 citation statements)

References 44 publications

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“…It was found that recoveries of Ppy (21h) /GOx/AuNPs/GR electrodes were in the range of 97–99 %, which is similar to results obtained with electrode modified with AuNP, 1,10‐phenanthroline‐5,6‐dione and Ppy(22h) layer (98 %) or higher in comparison to electrode based on GOx immobilised on an overoxidized Ppy film decorated with electrochemically deposited AuNP (96 %) . Therefore, the developed electrochemical biosensor based on Ppy (21h) /GOx/AuNPs/GR electrode followed by amperometric glucose detection is applicable for the determination of glucose in human serum samples.…”

Section: Resultssupporting

confidence: 74%

Amperometric Glucose Biosensor Based on Electrochemically Deposited Gold Nanoparticles Covered by Polypyrrole

2017

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Graphite rod (GR) modified with electrochemicaly deposited gold nanoparticles (AuNPs) and adsorbed glucose oxidase (GOx) was used in amperometric glucose biosensor design. Enzymatic formation of polypyrrole (Ppy) on the surface of GOx/AuNPs/GR electrode was applied in order to improve analytical characteristics and stability of developed biosensor. The linear glucose detection range for Ppy/GOx/AuNPs/GR electrode was dependent on the duration of Ppy‐layer formation and the linear interval was extended up to 19.9 mmol L−1 after 21 h lasting synthesis of Ppy. The sensitivity of the developed biosensor was determined as 21.7 μA mM−1 cm−2, the limit of detection – 0.20 mmol L−1. Ppy/GOx/AuNPs/GR electrodes demonstrated advanced good stability (the t1/2 was 9.8 days), quick detection of glucose (within 5 s) in the wide linear interval. Additionally, formed Ppy layer decreased the influence of electroactive species on the analytical signal. Developed biosensor is suitable for the determination of glucose in human serum samples.

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Section: Resultssupporting

confidence: 74%

Amperometric Glucose Biosensor Based on Electrochemically Deposited Gold Nanoparticles Covered by Polypyrrole

2017

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“…A tangible application of amperometric immunosensor is for the measurement of β‐HCG level in advanced pregnancy testing . In another work, German et al established a glucose‐based amperometric sensor by utilizing gold nanoparticle along with electron transfer mediator and different immobilized glucose oxidase that were sourced from Penicillium funiculosum, Penicillium adametzii , or Aspergillus niger that were cross‐linked with glutaraldehyde . They observed a linear concentration range and the glucose oxidase from Penicillium funiculosum exhibited a limit of detection of 0.024 mmol L −1 .…”

Section: Biosensorsmentioning

confidence: 99%

Progress in the Development of Intrinsically Conducting Polymer Composites as Biosensors

Prajapati

Kandasubramanian

2019

Macro Chemistry & Physics

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Biosensors are analytical devices which find extensive applications in fields such as the food industry, defense sector, environmental monitoring, and in clinical diagnosis. Similarly, intrinsically conducting polymers (ICPs) and their composites have lured immense interest in bio‐sensing due to their various attributes like compatibility with biological molecules, efficient electron transfer upon biochemical reactions, loading of bio‐reagent, and immobilization of biomolecules. Further, they are proficient in sensing diverse biological species and compounds like glucose (detection limit ≈0.18 nm), DNA (≈10 pm), cholesterol (≈1 µm), aptamer (≈0.8 pm), and also cancer cells (≈5 pm mL−1) making them a potential candidate for biological sensing functions. ICPs and their composites have been extensively exploited by researchers in the field of biosensors owing to these peculiarities; however, no consolidated literature on the usage of conducting polymer composites for biosensing functions is available. This review extensively elucidates on ICP composites and doped conjugated polymers for biosensing functions of copious biological species. In addition, a brief overview is provided on various forms of biosensors, their sensing mechanisms, and various methods of immobilizing biological species along with the life cycle assessment of biosensors for various biosensing applications, and their cost analysis.

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“…Nanoparticles were used to enhance glucose oxidase electrode in the literature. 35,[43][44][45][46] German The effects of enzyme immobilization conditions such as Chi, GOD and GAL concentrations were also investigated and current responses were given for 3 mM glucose solution in the Fig. 5 A, B and C, respectively.…”

Section: The Effect Of Znonp Modification On Current Responsementioning

confidence: 99%

“…ZnO nanomaterials have been widely used for this purpose. [40][41][42][43] The present study deals with the fabrication of a glucose biosensor based on redox polymers such as PPy and POA. Construction of biosensor was optimized in terms of PPy and POA synthesis parameters, enzyme immobilization conditions and also the amount of ZnONP by comparing current response in the glucose solution.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles

Özyılmaz

Bayram

et al. 2019

ACSi

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GOD was immobilized onto polypyrrole (PPy) or poly(o-anisidine) (POA) coated Pt electrode to construct glucose sensitive biosensor. Because polymer film properties and enzyme activity affect the current response, PPy and POA synthesis conditions and also enzyme immobilization parameters were optimized in detail. The optimal monomer concentrations were determined as 25 and 50 mM for PPy and POA, respectively, whereas scan rate was 50 mV/s for both polymer films. In case of immobilization procedure, the optimal Chitosan (Chi), glucose oxidase (GOD) and glutaraldehyde (GAL) concentrations were determined as 0.5%, 2 mg/ml and 0.05% for PPy and 0.5%, 4 mg/ml and 0.075% for POA, respectively. Zinc oxide nanoparticles (ZnONP) were co-immobilized with GOD enzyme and it was revealed that ZnONP modification enhanced the efficiencies of both electrodes in terms of current responses and stabilities. Nyquist diagrams showed that enzyme electrodes were sensitive to glucose molecule and ZnONP modification improved the sensor efficiency.

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The use of different glucose oxidases for the development of an amperometric reagentless glucose biosensor based on gold nanoparticles covered by polypyrrole

Cited by 64 publications

References 44 publications

Amperometric Glucose Biosensor Based on Electrochemically Deposited Gold Nanoparticles Covered by Polypyrrole

Amperometric Glucose Biosensor Based on Electrochemically Deposited Gold Nanoparticles Covered by Polypyrrole

Progress in the Development of Intrinsically Conducting Polymer Composites as Biosensors

Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles

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