The use of cobalt-induced chlorophyll morphoses for studying Co2+ interactions with cysteine and SOD

Kleizaitė, Violeta; Čėsnienė, Tatjana; Rančelis, Vytautas

doi:10.1016/j.plantsci.2004.06.023

Cited by 11 publications

(9 citation statements)

References 32 publications

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“…Kleizaite et al [2] observed similar trends due to application of heavy metals like copper, zinc, mercury, lead and cadmium in rice. Further increase in Co level decreased the amino acid and protein contents.…”

Section: Biochemical Constituentsmentioning

confidence: 77%

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Low Concentration of Cobalt Increases Growth, Biochemical Constituents, Mineral Status and Yield in Zea Mays

et al. 2008

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A pot culture experiment has been carried out to find the extent of changes occurring in the biochemicals and nutrients of maize plants (Zea mays L.) grown under different concentrations of cobalt (50, 100, 150, 200, 250 mg kg -1 soil). The growth and yield parameters such as seedling vigour, number of cobs, number of seeds per plant; photosynthetic pigments viz., chlorophyll 'a', chlorophyll 'b', and total chlorophyll contents; biochemicals like total sugars (reducing and non reducing), starch, amino acids and protein content and various macro-and micronutrients are determined 90 days after sowing (DAS). All the growth parameters, pigment content, biochemicals and mineral content increase at 50 mg Co kg -1 soil when compared with the control. Further increase in the Co levels (100-200 mg kg -1 soil) has a negative effect on all the above parameters.

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Section: Biochemical Constituentsmentioning

confidence: 77%

“…Excessive metal concentrations in contaminated soils can result in decreased soil microbial activity and soil fertility and crop yield losses [1]. Accumulation of trace elements, especially heavy metals, in the soil has potential to restrict the soil's function, cause toxicity to plants, and contaminate the food chain [2].…”

Section: Introductionmentioning

confidence: 99%

Low Concentration of Cobalt Increases Growth, Biochemical Constituents, Mineral Status and Yield in Zea Mays

et al. 2008

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“…Normally, the optimal temperature for V. faba is 23–25°C. Conditions for CM induction were elaborated in our previous works (Barysas et al, 2002; Kleizaite et al, 2004; Cesniene et al, 2005; Rancelis et al, 2006). Only seed treatment with Co(NO 3 ) 2 solutions in the interval of 2.5–10 mM induces CMs in V. faba .…”

Section: Methodsmentioning

confidence: 99%

“…The homogenate was centrifuged at 12 000 × g for 15 min at 4°C. The supernatant was used for nondenaturating polyacrilamide gel electrophoresis (PAGE) according to the procedure used in the previous work (Kleizaite et al, 2004). SOD isozymes were detected in gels by the nitro blue tetrazolium (NBT) reduction method (Beauchamp and Fridovich, 1971).…”

Section: Methodsmentioning

confidence: 99%

“…Investigation of SOD isoforms in V. faba revealed not only a significant individual but also Co induced polymorphism, especially for Cu/Zn SOD. As the SOD polymorphism is of phenotypical character, replacement interactions were supposed to occur (Kleizaite et al, 2004). However, SOD is also regulated epigenetically (Hurt et al, 2007; Dugas and Bartel, 2008), and epigenetic processes may also contribute to SOD polymorphism in V. faba plants.…”

Section: Introductionmentioning

confidence: 99%

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Influence of cobalt uptake by Vicia faba seeds on chlorophyll morphosis induction, SOD polymorphism, and DNA methylation

Rančelis

Čėsnienė

Kleizaitė

et al. 2011

Environmental Toxicology

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Vicia faba plants show polymorphism to cobalt (Co) excess, expressed by a different degree of chlorophyll morphosis (CM)-from normally green (N) to yellow (Y) seedlings. For superoxide dismutase (SOD), the high V. faba polymorphism was revealed and increased by Co stress. Epigenetic mechanisms may be involved in both phenomena. For such reasons, we investigated the effect of 5-azacytosine (AzaC) and Na butyrate (NaBut) on CM induction, SOD polymorphism, and DNA methylation-demethylation events in Co(NO(3) )(2) affected plants, without or with AzaC or NaBut. CMs were induced after treatment of seeds for 8 h with 7.5 mM Co(NO(3) )(2) plus 12 h with H(2) O or 8 h with H(2) O plus 12 h with Co(NO(3) )(2) . In the same order AzaC and NaBut were applied in concentrations equimolar to Co(NO(3) )(2) . SOD isoforms were investigated electrophoretically, and for DNA methylation-demethylation events the Aina [Aina et al. (2004) Physiol Plant 121:472-480] system was applied upon using the random amplified polymorphic DNA (RAPD) method employing restrictases MspI and HpaII. The effect of AzaC and NaBut on CM induction in combination with Co was unclear. Posttreatment with Co was more effective than Co-pretreatment. SOD polymorphism was significantly strengthened by NaBut. Detection of DNA methylation-demethylation events depended on the primers used for RAPD analysis. With AP5 and MP4 primers, DNA demethylation was observed in N-seedlings after exposure to Co, AzaC or NaBut applied separately. With primer A6, only DNA methylation events were determined in N-seedlings from seeds exposed to Co or Co-AzaC, and in Y-seedlings after Co-AzaC or Co-NaBut treatment. UPGMA grouping of the results showed that all N-seedlings comprised one common cluster after Co exposure, independently of treatment combinations (Co alone, Co with AzaC, Co with NaBut). On the contrary, no significant differences were determined in SOD polymorphism among the most resistant N-seedlings and the most severely affected Y-seedlings.

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Excess nickel–induced changes in antioxidative processes in maize leaves

Kumar

Tewari

Sharma

2007

J. Plant Nutr. Soil Sci.

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Maize (Zea mays L. cv. 777) plants grown in hydroponic culture were treated with 100 µM NiSO4 (moderate nickel (Ni) excess). In addition to growth parameters, metabolic parameters representative of antioxidant responses in leaves were assessed 24 h and 3, 7, and 14 d after initiating the Ni treatment. Extent of oxidative damage was measured as accumulation of malondialdehyde and hydrogen peroxide in leaves 7 and 14 d after treatment initiation. Apart from increasing membrane‐lipid peroxidation and H2O2 accumulation, excess supply of Ni suppressed plant growth and dry mass of shoots but increased dry mass of roots and decreased the concentrations of chloroplastic pigments. Excess supply of Ni, though inhibited the catalase (EC 1.11.1.6) activity, increased peroxidase (EC 1.11.1.7), ascorbate peroxidase (EC 1.11.1.11), and superoxide dismutase (EC 1.15.1.1) activities. Localization of isoforms of these enzymes (peroxidase, ascorbate peroxidase, and superoxide dismutase) on native gels also revealed increases in the intensities of pre‐existing bands. Enhanced activities of peroxidase, ascorbate peroxidase, and superoxide dismutase, however, did not appear to be sufficient to ameliorate the effects of excessively generated reactive oxygen species due to excess supply of Ni.

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The use of cobalt-induced chlorophyll morphoses for studying Co2+ interactions with cysteine and SOD

Cited by 11 publications

References 32 publications

Low Concentration of Cobalt Increases Growth, Biochemical Constituents, Mineral Status and Yield in Zea Mays

Low Concentration of Cobalt Increases Growth, Biochemical Constituents, Mineral Status and Yield in Zea Mays

Influence of cobalt uptake by Vicia faba seeds on chlorophyll morphosis induction, SOD polymorphism, and DNA methylation

Excess nickel–induced changes in antioxidative processes in maize leaves

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