The use of coated paramagnetic particles as a physical label in a magneto-immunoassay

Richardson, Julie; Hawkins, Peter; Luxton, Richard

doi:10.1016/s0956-5663(01)00201-9

Cited by 86 publications

(37 citation statements)

References 9 publications

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“…Second, the signal is quantitative and can be assigned a value in millivolts. Third, the signal generated is comparable to the detection limits seen with radionucleotide labels or nephelometric techniques (70,71).…”

Section: Immunochromatographic Lateral Flow Assaysmentioning

confidence: 63%

“…Their broad potential for laboratory applications has been demonstrated by their use in detection of typhoid-specific antibodies, cell separation, and antibody sorting (53,(88)(89)(90). More recently, systems using paramagnetic particle labels have been described that make it possible to use iron oxide particles as labels in place of gold (6,49,70,71). The labeled antibodyantigen mixture wicks up the membrane, as described above, and is deposited at the site of the solid-phase antibody, and the magnetic flux is measured in the antigen capture zone.…”

Section: Immunochromatographic Lateral Flow Assaysmentioning

confidence: 99%

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Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents

Peruski

2003

Clin Vaccine Immunol

195

119

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NATURE OF THE PROBLEMBW agents. The release of a biological weapon (BW) agent by a terrorist group or military force would likely be silent and undetectable or nearly so. As shown by anthrax attack during the fall of 2001 in the eastern United States, patients would begin appearing at hospitals and clinics within several days of exposure, most presenting with nonspecific flu-like symptoms. The first days of the outbreak might not even cause undue concern. However, depending on the type of agent and the method of dispersal, the public healthcare system would rapidly be stretched to capacity and beyond.The qualities that make a good BW agent are its relationship between aerosolization, infectivity, or toxicity and the amount of agent required to produce an effect (48). In addition, criteria such as environmental stability, ease of production, disease severity, and communicability determine which agents are the most likely to be utilized. For maximum effect, an optimal agent should be highly lethal and easily produced in large quantities and have limited options for preventive or prophylactic treatment. Given that the respiratory route is the most effective for most BW agents, stability in an aerosol form and the capability to be readily dispersed also in an aerosol (1-to 10-m particle size) are necessary. When potential agents are reviewed for these characteristics, Bacillus anthracis (anthrax) and variola major virus (smallpox) are considered to have the greatest potential for mass casualties and civil disruption. Also high on a prospective list of agents are botulinum neurotoxins, Yersinia pestis, and Francisella tularensis (48,91,92). Lower on the prospective list are Burkholderia pseudomallei and Burkholderia mallei, Rickettsia sp., Coxiella burnetii, Venezuelan equine encephalitis virus, Marburg and Ebola viruses, and influenza viruses (48,63,91,92).Emerging infectious disease agents. In addition to diseases caused by intentional epidemics, there are several emerging infectious diseases (ID) with the potential for significant public health consequences, including dengue fever, West Nile fever, and Rift Valley fever as well as the recent reemergence of malaria in the eastern United States (48,63,91,92). As with BW agents, emerging ID agents may be directly transmissible or vector borne (63). A complex interplay of factors can influence disease emergence, including genetic variation, environmental changes, and population pressures. Further compounding this already complicated situation, are the estimated 600 million international tourists annually, many with the potential to the spread disease globally in a matter of hours (63). Clearly, the challenges facing modern clinical microbiologists and immunologists are daunting enough without the added difficulties posed by the intentional release of BW/ID agents! Because of the threat posed by both BW and emerging or reemerging ID agents, there is a need to rapidly identify such agents in the clinical setting in order to treat the individuals at risk and to improve p...

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Section: Immunochromatographic Lateral Flow Assaysmentioning

confidence: 63%

Section: Immunochromatographic Lateral Flow Assaysmentioning

confidence: 99%

Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents

Peruski

2003

Clin Vaccine Immunol

195

119

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“…[4][5][6] Magnetite nanoparticles can also rapidly separate the target molecules from the samples by simply applying an appropriate magnetic field. They have broad applications in biology and biomedicine, including drug delivery and targeting, [7][8][9][10][11][12] immunoassays, [13] hyperthermia, [14] magnetic resonance imaging contrast enhancements, [15] biosensing, [16] and the separation, purification or detection of proteins, DNA, viruses, and cells, etc. [17][18][19][20] For biological applications, many researchers have prepared Fe 3 O 4 magnetic nanoparticles with various structures, or loaded them with silica, [21] polymer, [22] and lipid vesicles, [23] etc.…”

Section: Introductionmentioning

confidence: 99%

Polyhedral Magnetite Nanocrystals with Multiple Facets: Facile Synthesis, Structural Modelling, Magnetic Properties and Application for High Capacity Lithium Storage

Horvat

Munroe

et al. 2011

Chemistry A European J

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Polyhedral magnetite nanocrystals with multiple facets were synthesised by a low temperature hydrothermal method. Atomistic simulation and calculations on surface attachment energy successfully predicted the polyhedral structure of magnetite nanocrystals with multiple facets. X-ray diffraction, field emission scanning electron microscopy, and high resolution transmission microscopy confirmed the crystal structure of magnetite, which is consistent with the theoretical modelling. The magnetic property measurements show the superspin glass state of the polyhedral nanocrystals, which could originate from the nanometer size of individual single crystals. When applied as an anode material in lithium ion cells, magnetite nanocrystals demonstrated an outstanding electrochemical performance with a high lithium storage capacity, a satisfactory cyclability, and an excellent high rate capacity.

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“…MBs exhibit a large active surface area which enables the immobilization of high immunoreagent loadings onto the solid phase of the electrochemical transducer by applying an external magnetic field, thus leading to an increased sensitivity [21]. A decrease of matrix effect has been also claimed as a consequence of using MBs.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive determination of human growth hormone (hGH) with a disposable electrochemical magneto-immunosensor

et al. 2012

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In this paper, an electrochemical magneto-immunosensor for the detection of human growth hormone (hGH) is described for the first time. The immunosensor involves the use of tosyl-activated magnetic microparticles (TsMBs) to covalently immobilize a monoclonal mAbhHG antibody. A sandwich-type immunoassay with a secondary pAbhGH antibody and anti-IgG labelled with alkaline phosphatase (anti-IgG-AP) was employed. TsMBs–mAbhGH–hGH–pAbhGH–anti-IgG-AP conjugates were deposited onto the surface of a screen-printed gold electrode using a small neodymium magnet, and electrochemical detection was performed by square-wave voltammetry upon the addition of 4-aminophenyl phosphate as the AP substrate. All the variables involved in the preparation of immunoconjugates and in the immunoassay protocol were optimized. A calibration curve for hGH was constructed with a linear range between 0.01 and 100 ng/mL (r = 0.998) and a limit of detection of 0.005 ng/mL. This value is nearly three orders of magnitude lower than that obtained using surface plasmon resonance (Treviño et al., Talanta 78:1011-1016, 2009). Furthermore, good repeatability, with RSD = 3% (n = 10) at the 1-ng/mL hGH level, was obtained. Cross-reactivity studies with other hormones demonstrated good selectivity. The magneto-immunosensor was applied to the analysis of human serum spiked with hGH at the 4- and 0.1-ng/mL levels. Mean recoveries of 96 ± 6% and 99 ± 2%, respectively, were obtained.

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The use of coated paramagnetic particles as a physical label in a magneto-immunoassay

Cited by 86 publications

References 9 publications

Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents

Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents

Polyhedral Magnetite Nanocrystals with Multiple Facets: Facile Synthesis, Structural Modelling, Magnetic Properties and Application for High Capacity Lithium Storage

Ultrasensitive determination of human growth hormone (hGH) with a disposable electrochemical magneto-immunosensor

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