The Use of C<sup>14</sup>-Proline by Growing Cell: Its Conversion to Protein and Hydroxyproline

Pollard, J.K.; Steward, F. C.

doi:10.1093/jxb/10.1.17

Cited by 67 publications

(16 citation statements)

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“…(17) showed that a 60-minute pretreatment of oat coleoptile sections with ribonuclease made them unresponsive to IAA within the next 60 minutes. In onion roots ribonuclease produced almost total suppression of protein synthesis in the cytoplasm, probably through destruction of the transfer RNA needed for protein synthesis (12 (32) and it is evidently incorporated to a very small extent, if at all, into the protein of carrot tissue explants (24). Similar studies with some of the antibiotics known.…”

Section: Resultsmentioning

confidence: 70%

Inhibition of protein synthesis and of auxin-induced growth by chloramphenicol.

Noodén¹,

Thimann²

1965

Plant Physiol.

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Section: Resultsmentioning

confidence: 70%

Inhibition of protein synthesis and of auxin-induced growth by chloramphenicol.

Noodén¹,

Thimann²

1965

Plant Physiol.

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“…This mode of action seemed uinlikely for hydroxyproline in light of the evidence obtained from both plant (9) and animal systems (13) fig 1). OnIl the r--isomers are growth inhibitors with the cis form (allohydroxyproline) being a more effecti-e inhibitor than the tr(nils form (hydroxyproline).…”

mentioning

confidence: 79%

Inhibition of Cell Elongation in Avena Coleoptile by Hydroxyproline

Cleland¹

1967

Plant Physiol.

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Summiiiiary. A sttudy has been -made of the hydroxyproline-induiced inhibition of elongation of Avena coleoptile tissues. The isomers of 4-hydroxyproline differ in their effectiveness; only the L isomers iare growth inhibitors with the cis form (allohydroxyproline) being more effective than the trans form (hydroxyproline).Hydroxyproline differs from other amino acid antagonists and protein synthesis inhibitors in respect to 2 characteristics of the growth inhibition. First, a certain increment of auxin-induced elongation must take place following addition of hydroxyproline before the growth is inhibited. In contrast, pretreatment with other amino acid antagonists or protein synthesis inhibitors completely eliminates the ability of Avena coleoptile sections to respond to auxin. Secondly, sucrose markedly increases the magnitude of the hydroxyproline inhibition; i.e., sucrose acts to inhibit rather than promote growth when in the presence of hydroxyproline.It appears that hydroxyproline is a specific inhibitor for the synthesis of some factor which is utilized in elongation. Following addition of hydroxyprolline, auxininduced elongation contintues until the pool of this factor is exhausted; then elongation is inhibited.Auxiin-iniduiced growth can be inhibited by a wide variety of agents (3). One group of inhibitors are the amino acid antagonists. Both division and elongation of plant cells are inhibited by antagonists such as ethionine (2, 12, 14), canavanine (1) and p-fluorophenylalanine (8).In 1958, Steward et al. (14) reported that the free amino acid hydroxyproline wotuld inhibit the growth of carrot callus tissues. Cleland extended this resuilt by showing that cell elongation in Avena coleoptile sections was also blocked by hydroxyproline (4). In both cases, hydroxyproline seemed to be acting as an antagonist of proline metabolism since the addition of proline completely reversed the hydroxyproline-indtuced inhibition.Many amino acid antagonists act by being incorporated into proteins in place of their corresponding amino acid with the result that inactive proteins are formed (7, 10). This mode of action seemed uinlikely for hydroxyproline in light of the evidence obtained from both plant (9) and animal systems (13) that free hydroxyproline is not incorporated into proteins. In order to determine the mode of action of hydroxyproline on cell elongation, an investigation of the effects of hydroxyproline on growth and metabolism has been uindertaken. This paper extends the earlier ob-I Surnorted by United States Public Health Service Granlt GM-12881. Materials and MethodsAzenaz seedlings were grown as detailed earlier (2). Sections 5 mm in length were cuit from the region 3 to 8 mm below the tip of 2.5 to 3.25 cm long coleoptiles. Grouips of 10 sections were placed in test tubes with 5 ml of soltution. The test tubes were rotated at 1 rpm on a Rollardruim and after the desired length of time, the sections were meastired with a microscope fitted with an eyepiece micrometer. All manipulations and incuibations we...

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“…Steward et al have reported that bound hydroxyproline is an end product of metabolism in cultured carrot cells and potato disks and that proline is converted to hydroxyproline after the proline has been bound, presumably into protein (11,16,19). Manner and Gould (12) working with chick embryos have presented results which suggest proline is hydroxylated prior to its assembly into collagen.…”

Section: Discussionmentioning

confidence: 99%

Proteins and Plant Cell Walls. Proline to Hydroxyproline in Tobacco Suspension Cultures

Olson

1964

Plant Physiol.

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The Use of C¹⁴-Proline by Growing Cell: Its Conversion to Protein and Hydroxyproline

Cited by 67 publications

References 0 publications

Inhibition of protein synthesis and of auxin-induced growth by chloramphenicol.

Inhibition of protein synthesis and of auxin-induced growth by chloramphenicol.

Inhibition of Cell Elongation in Avena Coleoptile by Hydroxyproline

Proteins and Plant Cell Walls. Proline to Hydroxyproline in Tobacco Suspension Cultures

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The Use of C14-Proline by Growing Cell: Its Conversion to Protein and Hydroxyproline

Cited by 67 publications

References 0 publications

Inhibition of protein synthesis and of auxin-induced growth by chloramphenicol.

Inhibition of protein synthesis and of auxin-induced growth by chloramphenicol.

Inhibition of Cell Elongation in Avena Coleoptile by Hydroxyproline

Proteins and Plant Cell Walls. Proline to Hydroxyproline in Tobacco Suspension Cultures

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The Use of C¹⁴-Proline by Growing Cell: Its Conversion to Protein and Hydroxyproline