The use of Acinetobacter sp. for oil hydrocarbon degradation in saline waters

Abstract

This paper describes the problem of oil pollutant re moval and the use of Acinetobacter genus microor ganisms in oil biodegradation. As part of the experi ments, Acinetobacter genus microorganism strain with the best ability to degrade crude oil and fuel oil in saline waters was selected from the microorganism collection of JSC "Biocentras". The impact of environmental con ditions (salinity, temperature, pH, substrate concent ration) on this strain's viability and the efficacy of its oil degradation processes were evaluated. The most ef ficient degradation of oil products by Acinetobacter ge nus bacteria was at temperature of 30 °C, in a pH range of 7-8, in model ocean water of 35 ‰ salinity.

Bioremediation of polycyclic aromatic hydrocarbons by Acinetobacter species isolated from ecological sources

“…Also, a decrease in the absorbance of UV-visible spectrum of PAH incubated separately with each species of Acinetobacter gives another evidence of degradation of PAH by the two studied species; this indicates that degradation of PAH occurred as bacteria uses it as carbon source and energy. It is worthwhile to mention that oil-degrading Acinetobacter produces an extracellular polyanionic, heteropolysaccharide bioemulsifier termed emulsan consisting of high-molecular-mass polysaccharides associated with proteins which also stabilizes oil-water and water-oil emulsions (Toren et al, 2001;Margesin et al, 2013;Fatajeva et al, 2014 ). The study also revealed that using mineral medium without addition of nutrients was adequate for enhancing bacterial growth and degradation activity of both species of Acinetobacter.…”

“…Zaki et al (2015) reported that crude oil degradation is faster in warm water because the heat generated within the water body will further encourage the breakdown of the spilled petroleum through natural processes such as evaporation. Besides, Fatajeva et al (2014) reported that H.T. Al-Hadithi et al Acinetobacter is the most active bacteria to degrade crude oil and fuel oil at a temperature of 30 °C, in a pH range of 7-8, in model ocean water of 35 ‰ salinity.…”

“…were identified from contaminated soil used as a nutrient source for ex situ remediation studies (Das and Chandran, 2011;Chikere, 2012). Fatajeva et al (2014) recently reported that Acinetobacter had high potential to degrade crude oil and fuel in saline water. The highest degredation of oil was found at 30 °C, pH 7-8 in model ocean water of 35 % salinity.…”

Biodegradation of fenoxaprop-P-ethyl (FE) by Acinetobacter sp. strain DL-2 and cloning of FE hydrolase gene afeH

“…There are many reports about degradation of environmental pollutants by Acinetobacter sp. (Fatajeva et al, 2014;Kim et al, 2003;Abdel-El-Haleem, 2004), but FE-degrading strain was isolated from the genus for the first time.…”

Biodegradation of Petroleum Hydrocarbons by Actinobacteria and Acinetobacteria Strains Producing Biosurfactant

“…Bioremediation of polluted environments is based on contaminant biodegradation, that is, metabolic abilities of microorganisms to transform or mineralize organic contaminants into less harmful, nonhazardous substances, which are further integrated into natural biogeochemical cycles [5,14,20]. The spectrum of microorganisms used for hydrocarbons degradation mostly includes the actinobacteria of Rhodococcus, Dietzia, Gordonia genera as well as bacteria of Pseudomonas, Arthrobacter and Acinetobacter genera [7,12,14,15]. Many authors have determined that these microorganisms are widely spread in oil-polluted ecosystems where hydrocarbon-oxidizing actinobacteria play a significant role, which is explained by metabolic peculiarities of these bacteria and their resistance to unfavourable conditions [9,14].…”