The use of 3,3′,4′,5‐tetrachlorosalicylanilide as a chemical uncoupler to reduce activated sludge yield

Chen, Ying Xu; Ye, Fen Xia; Feng, Xiaoshan

doi:10.1002/jctb.937

Cited by 11 publications

(9 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The microbial activity in these systems was high compared to the control system. Chen et al (2004) tested different doses of TCS and verified that a higher TCS concentration (up to 3.6 mg/L) resulted in a more rapid oxygen uptake rate, thus providing higher levels of metabolic uncoupling. They concluded that the over-consumption of oxygen suggested the occurrence of a high level of energy dissipation for metabolic regulation, which could be the cause of the reduction in sludge growth.…”

Section: Respirometry Microscopy and Toxicity Resultsmentioning

confidence: 84%

Reduction of Excess Sludge Production in an Activated Sludge System Based on Lysis-Cryptic Growth, Uncoupling Metabolism and Folic Acid Addition

Velho

Daudt

Martins

et al. 2016

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

-The following sludge reduction alternatives were tested in wastewater biological reactors: oxic-settling-anaerobic (OSA-process); ultrasonic disintegration (UD); chlorination (CH); 3,3',4',5-tetrachlorosalicylanilide (TCS); and folic acid (FA). Compared to the control system, UD reduced 55% of the sludge production, and greater substrate and nutrient removal efficiency was achieved. CH worsened the sludge settleability and increased the SVI values; the system achieved 25% of sludge reduction. OSA showed 50% and 60% of sludge reduction after 16 and 10 hours under anaerobic conditions, respectively. The observed sludge yield during TCS addition was decreased by 40%, and the sludge settleability worsened. FA presented the highest sludge reduction (75%), and the system improved the nutrient removal efficiency by 30% compared to the control system and maintained the sludge properties. Acute toxicity conducted with Daphnia magna classified the effluent from the sludge reduction systems as non-toxic for discharge into water sources.

show abstract

Section: Respirometry Microscopy and Toxicity Resultsmentioning

confidence: 84%

Reduction of Excess Sludge Production in an Activated Sludge System Based on Lysis-Cryptic Growth, Uncoupling Metabolism and Folic Acid Addition

Velho

Daudt

Martins

et al. 2016

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

show abstract

“…Chen et al reported dehydrogenase activity of 10.04 mg TF/ mg MLSS h in an activated sludge system (Chen et al, 2004). It can be seen that the intracellular ATP concentration and dehydrogenase activity of aerobic zone were much higher than that of the previous studies.…”

Section: The Microbial Activity Of the Siper Systemmentioning

confidence: 87%

Investigation of microbial community structure in an advanced activated sludge side-stream reactor process with alkaline treatment

Yan

Wang²,

Chen

et al. 2015

International Biodeterioration & Biodegradation

View full text Add to dashboard Cite

“…It belongs to a class of chemicals termed as protonophores because of their ability to shuttle electrons across the cell membrane. A number of previous studies have shown TCS to be capable of reducing the biomass yield of activated sludge cultures, effectively at lower concentrations compared to other uncouplers (Chen et al, 2000(Chen et al, , 2002(Chen et al, , 2004Ye and Li, 2005;Ye et al, 2003).…”

Section: Introductionmentioning

confidence: 98%

“…Senez (1962) observed a roughly 50% reduction in the yield of Desulfovibrio desulfuricans (strain Berre S) under some growth conditions. It is now known that the presence of excess substrate, addition of organic protonophores, toxins such as heavy metals and antibiotics, and other thermal or physico-chemical processes can all lead to a partial dissociation between catabolism and anabolism (Chen et al, 2004;Cook and Russell, 1994;Liu, 1998;Mayhew and Stephenson, 1997;Wei et al, 2003). This process is generically termed metabolic uncoupling, and it is characterized by a decrease in the biomass yield.…”

Section: Introductionmentioning

confidence: 98%

Metabolic uncoupling of Shewanella oneidensis MR‐1, under the influence of excess substrate and 3, 3′, 4′, 5‐tetrachlorosalicylanilide (TCS)

Saini

Wood

2007

Biotech & Bioengineering

View full text Add to dashboard Cite

ABSTRACT:The dissociation between catabolism and anabolism is generally termed as metabolic uncoupling. Experimentally, metabolic uncoupling is characterized by a reduction in the observed biomass yield. This condition can be brought about by: (a) excess substrate (as measured by S 0 /X 0 ), and (b) addition of chemical uncouplers such as 3, 3 0 , 4 0 , 5-Tetrachlorosalicylanilide (TCS). An empirical model is proposed to quantify the uncoupling effects of both excess substrate and uncoupler addition on the microbial cultures. Metabolic uncoupling of Shewanella oneidensis MR-1, under the influence of excess pyruvate and TCS, has been modeled using the proposed expression. The degree of uncoupling was measured as a fractional reduction in theoretical maximum observed yield. Excess substrate was observed to successively reduce biomass yield as substrate concentration was increased. In the presence of TCS, conflicting trends were obtained for number yield and protein yield. This could, in part, be attributed to the observed increase in cellular protein content upon addition of TCS. Excess substrate conditions dominated uncoupling, as compared to uncoupler addition. However, these two approaches were found to have additive effects and could, in conjunction, be employed to control biomass growth during microbial processes such as subsurface bioremediation and activated sludge treatment.

show abstract

The use of 3,3′,4′,5‐tetrachlorosalicylanilide as a chemical uncoupler to reduce activated sludge yield

Cited by 11 publications

References 14 publications

Reduction of Excess Sludge Production in an Activated Sludge System Based on Lysis-Cryptic Growth, Uncoupling Metabolism and Folic Acid Addition

Reduction of Excess Sludge Production in an Activated Sludge System Based on Lysis-Cryptic Growth, Uncoupling Metabolism and Folic Acid Addition

Investigation of microbial community structure in an advanced activated sludge side-stream reactor process with alkaline treatment

Metabolic uncoupling of Shewanella oneidensis MR‐1, under the influence of excess substrate and 3, 3′, 4′, 5‐tetrachlorosalicylanilide (TCS)

Contact Info

Product

Resources

About