The upregulation of specific interleukin (IL) receptor antagonists and paradoxical enhancement of neuronal apoptosis due to electrode induced strain and brain micromotion

Karumbaiah, Lohitash; Norman, Sharon; Rajan, Nithish B.; Anand, Sanjay; Saxena, Tarun; Betancur, Martha I.; Patkar, Radhika; Bellamkonda, Ravi V.

doi:10.1016/j.biomaterials.2012.05.021

Cited by 91 publications

(103 citation statements)

References 51 publications

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“…For all tests, p < 0.05 was considered significant. For the gene expression data, a greater than three-fold (AE3) change was considered significant [11].…”

Section: Discussionmentioning

confidence: 99%

“…3 days and 16 weeks post electrode implantation, animals were transcardially perfused with PBS, followed by 4% paraformaldehyde, followed by 30% sucrose in PBS (300 ml each) and brain tissue for immunohistochemical analysis (n ¼ 6/ electrode type) was sectioned as described previously [11]. For LCM, the electrodes were gently extracted after soaking overnight at 4 C in 30% sucrose in PBS, and the brains were snap frozen and stored at À80 C. Brain tissue was sectioned at a thickness of 15 mm using a cryostat, and serial sections were collected on polyethylene naphthalate (PEN) membrane slides (Life Technologies, NY) for LCM, and on charged glass slides (VWR, PA) for immunohistochemical analysis.…”

Section: Brain Tissue Preparation and Immunohistochemistrymentioning

confidence: 99%

“…To probe the abundance of transcripts encoding various myeloid cells activating, BBB regulating cytokines and matrix metalloproteinases (Table 1), RNA extraction, quantification, and gene expression assays were run according to methods previously described [11]. Expression data was normalized against the average expression of two endogenous housekeeping geneseGAPDH and HPRT.…”

Section: Qrt-pcr Array Analysismentioning

confidence: 99%

“…Sections were allowed to air dry for 5 min on the ice block before proceeding directly to LCM. PEN membrane slides containing IgG stained 3 day and 16 week tissue sections were laser capture micro-dissected using an Arcturus XT Laser Capture Microdissection System (Life Technologies, NY), as described previously [11].…”

Section: Laser Capture Microdissectionmentioning

confidence: 99%

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The impact of chronic blood–brain barrier breach on intracortical electrode function

et al. 2013

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“…For all tests, p < 0.05 was considered significant. For the gene expression data, a greater than three-fold (AE3) change was considered significant [11].…”

Section: Discussionmentioning

confidence: 99%

Section: Brain Tissue Preparation and Immunohistochemistrymentioning

confidence: 99%

Section: Qrt-pcr Array Analysismentioning

confidence: 99%

Section: Laser Capture Microdissectionmentioning

confidence: 99%

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The impact of chronic blood–brain barrier breach on intracortical electrode function

et al. 2013

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“…20,2331,32 Furthermore, rigid electronic implants can alter the physiological movement of organs such as the heart [33][34][35] and neuronal tissue. [36][37][38] Therefore, numerous studies have been conducted to optimize flexibility and geometrical structure of different substrates for future implants. [39][40][41][42][43][44][45][46] However, adding electronic functionality to soft substrate materials remains difficult due to technical limitations arising from standard fabrication methods.…”

Section: Introductionmentioning

confidence: 99%

Printed microelectrode arrays on soft materials: from PDMS to hydrogels

et al. 2018

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Microelectrode arrays (MEAs) provide promising opportunities to study electrical signals in neuronal and cardiac cell networks, restore sensory function, or treat disorders of the nervous system. Nevertheless, most of the currently investigated devices rely on silicon or polymer materials, which neither physically mimic nor mechanically match the structure of living tissue, causing inflammatory response or loss of functionality. Here, we present a new method for developing soft MEAs as bioelectronic interfaces. The functional structures are directly deposited on PDMS-, agarose-, and gelatin-based substrates using ink-jet printing as a patterning tool. We demonstrate the versatility of this approach by printing high-resolution carbon MEAs on PDMS and hydrogels. The soft MEAs are used for in vitro extracellular recording of action potentials from cardiomyocyte-like HL-1 cells. Our results represent an important step toward the design of next-generation bioelectronic interfaces in a rapid prototyping approach.npj Flexible Electronics (2018) 2:15 ;

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A Materials Roadmap to Functional Neural Interface Design

Wellman

Eles

Ludwig

et al. 2017

Adv Funct Materials

299

328

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Advancement in neurotechnologies for electrophysiology, neurochemical sensing, neuromodulation, and optogenetics are revolutionizing scientific understanding of the brain while enabling treatments, cures, and preventative measures for a variety of neurological disorders. The grand challenge in neural interface engineering is to seamlessly integrate the interface between neurobiology and engineered technology, to record from and modulate neurons over chronic timescales. However, the biological inflammatory response to implants, neural degeneration, and long-term material stability diminish the quality of interface overtime. Recent advances in functional materials have been aimed at engineering solutions for chronic neural interfaces. Yet, the development and deployment of neural interfaces designed from novel materials have introduced new challenges that have largely avoided being addressed. Many engineering efforts that solely focus on optimizing individual probe design parameters, such as softness or flexibility, downplay critical multi-dimensional interactions between different physical properties of the device that contribute to overall performance and biocompatibility. Moreover, the use of these new materials present substantial new difficulties that must be addressed before regulatory approval for use in human patients will be achievable. In this review, the interdependence of different electrode components are highlighted to demonstrate the current materials-based challenges facing the field of neural interface engineering.

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The upregulation of specific interleukin (IL) receptor antagonists and paradoxical enhancement of neuronal apoptosis due to electrode induced strain and brain micromotion

Cited by 91 publications

References 51 publications

The impact of chronic blood–brain barrier breach on intracortical electrode function

The impact of chronic blood–brain barrier breach on intracortical electrode function

Printed microelectrode arrays on soft materials: from PDMS to hydrogels

A Materials Roadmap to Functional Neural Interface Design

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