The unsaturated fatty acid content of mitochondria in relation to oxidation of exogenous reduced nicotinamide adenine dinucleotide

Guarnieri, Michael; Johnson, Ralph M.; Du, Julie T.

doi:10.1016/0005-2728(71)90125-3

Cited by 9 publications

(2 citation statements)

References 9 publications

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“…Hazel (1972) found that the more unsaturated lipid from acclimatized goldfish (5° C) was a better reactivator of succinic dehydrogenase activity than that from goldfish kept at 25° C. Therefore, the higher degree of unsaturation of the phospholipids of chill-sensitive and chill-resistant organisms may allow greater activity of specific enzymes at chilling temperatures than would otherwise be possible. The degree of unsaturation of the phospholipids may control the metabolic activity of mitochondria by limiting the activity of succinic dehydrogenase, but this would appear unlikely as Guarnieri, Johnson and Du (1971) showed that the fatty-acid composition of mitochondrial membranes may vary considerably without a corresponding change in membrane enzymic activity.…”

Section: Changes In the Weights Of The Polar Lipids On Chillingmentioning

confidence: 99%

The Acclimatization of Plants to Chilling Temperatures in Relation to the Fatty‐acid Composition of Leaf Polar Lipids

Wilson

Crawford

1974

New Phytologist

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SUMMARYAn examination was made of the effects of low-temperature acclimatization on the fatty-acid composition of the polar lipids of two chill-sensitive species Gossypium hirsutum and Phaseolus vulgaris which harden readily against chilling injury. As controls, the effect of hardening temperatures on a chill-resistant species, Hordeum vulgare, and a chill-sensitive species, Episcia reptans, which did not harden were also investigated. The degree of unsaturation of the fatty acids associated with the phospholipid fraction increased during hardening of Gossypium hirsutum and Phaseolus vulgaris. Furthermore, the degree of unsaturation and weight of phospholipids decreased in the leaves of these species with increase in physiological age at 25° C and was related to the increased susceptibility of older leaves to chilling injury. The acclimatization treatment produced no effects on the fatty-acid composition of the glycolipids of these plants and this agreed with earlier observations which showed no increase in unsaturation of the total leaf fatty acid on hardening (Wilson and Crawford, 1974). Therefore, in leaves, it was the phospholipid fraction alone and representing only 22% of the total leaf fatty acids that had its degree of unsaturation positively related to the chilling tolerance of the species investigated. These findings are discussed in relation to similar ones made on the acclimatization of animals to low temperatures.

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Section: Changes In the Weights Of The Polar Lipids On Chillingmentioning

confidence: 99%

The Acclimatization of Plants to Chilling Temperatures in Relation to the Fatty‐acid Composition of Leaf Polar Lipids

Wilson

Crawford

1974

New Phytologist

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“…A number of hypotheses have been proposed to explain this disparity. ALA may be more rapidly metabolized to its products or substantially oxidized by the liver (5,6).…”

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confidence: 99%

Incorporation of α‐linolenic acid and linoleic acid into human respiratory epithelial cell lines

Bryan

Hart

Forsyth

et al. 2001

Lipids

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Animal and human studies designed to examine the effects of alpha-linolenic acid (ALA) and linoleic acid (LA) supplementation on the fatty acid composition of plasma and tissues have demonstrated a marked difference in incorporation into phospholipids of these 18-carbon precursors of the long-chain polyunsaturates. Whereas tissue phospholipid levels are linearly related to dietary ALA and LA, the levels of tissue LA can be 10-fold higher than tissue ALA even when dietary levels are equivalent. There is some dispute whether this disparity is due to ALA being more rapidly metabolized to its products or substantially oxidized by the liver, or whether LA but not ALA is readily incorporated into cellular phospholipids. We examined the level of incorporation of polyunsaturated fatty acids into human respiratory epithelial cell lines (A549, 16HBE) by determining the dose-dependent incorporation of ALA and LA as free fatty acid (5-150 microg FFA/mL). Cell membrane phospholipid ALA and LA were both increased up to approximately 20-30% total fatty acids, with a concomitant decrease predominantly in monounsaturated membrane fatty acids, before significant toxicity was observed (50 microg/mL). Our data support the concept that rather than any inherent inability by human cells to incorporate ALA into membrane phospholipids, the lack of ALA content in human and animal tissues in vivo is due to the rapid metabolism or oxidation of this fatty acid in the liver.

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The lipid composition of flight muscle mitochondria isolated from the blowfly, Phormia regina

Guarnieri

Nair

Sacktor

1976

Archives of Biochemistry and Biophysics

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The unsaturated fatty acid content of mitochondria in relation to oxidation of exogenous reduced nicotinamide adenine dinucleotide

Cited by 9 publications

References 9 publications

The Acclimatization of Plants to Chilling Temperatures in Relation to the Fatty‐acid Composition of Leaf Polar Lipids

The Acclimatization of Plants to Chilling Temperatures in Relation to the Fatty‐acid Composition of Leaf Polar Lipids

Incorporation of α‐linolenic acid and linoleic acid into human respiratory epithelial cell lines

The lipid composition of flight muscle mitochondria isolated from the blowfly, Phormia regina

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