The ultrastructure ofCandida albicansafterin vitrotreatment with miconazole

Abstract

Electron microscopic examination on Candida albicans yeast cells after exposure in vitro to different doses ofmiconazole revealed that it exerts its effect primarily on the plasmalemma and the cell wall. After exposing cultures to drug concentrations of 10-nM and 10-sM progressive degradation of plasmalemma and cytoplasmic organelles were seen. Injured parts of cellular material were sequestered from the rest of the cytoplasm and extruded into the central vacuole. A similar degradation occurred at the cell periphery. Cell death, characterized by the loss of normal shape, dissolution of the plasmalemma and by severe alterations of all cytoplasmic organelles, was evident at the 5 × 10-nM and 10-SM dose levels. From the morphologic point of view a clear dose relationship could be established.

The effects of amorolfine and oxiconazole on the ultrastructure of Trichophyton mentagrophytes. A comparison

“…The ultrastructural damage caused by azoles is well documented [8][9][10][11] and has been reviewed by MUller [12]. The ultrastructural oxiconazole-related changes observed in the present study are qualitatively the same as found with amorolfine.…”

Mechanism of Fungal Growth Suppression by Inhibitors of Ergosterol Biosynthesis

“…Yet, alteration of cell wall structure has been detected with scanning and transmission electron microscopy (68,69).…”

Aspects of the effect of bile salts onCandida albicans

“…Econazole, fenticonazole, isoconazole and miconazole all caused convolutions and wrinkles in the cell wall [6]. The folds in the cell walls of miconazole-treated cells may correspond to focal areas where membranous material had been deposited between the cell wall and the plasmalemma [8,10]. Another feature of the action of miconazole is that chains of inter-connected cells were observed with pronounced necks [9] and this was also observed with cholic acid-treated cells (Fig.…”