The effects of amorolfine and oxiconazole on the ultrastructure of <i>Trichophyton mentagrophytes</i>. A comparison

Melchinger, Wolfgang; Polak, Annemarie; Müller, J.

doi:10.1111/myc.1990.33.7-8.393

Cited by 12 publications

(2 citation statements)

References 8 publications

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“…5). Similar findings were described by Melchinger et al (1990) and Figueras et al (1995), which demonstrated that these electron-dense materials were due to the leakage of cytoplasmic constituents in the course of the damaging process, but not attribute to the preparation techniques of TEM. From our point of view, the fibrillar materials were most probably related to be the initial stages of CF66I-cell interactions or with the preliminary step of cell destruction, which could explain why it was almost absent in the completely destroyed hyphae (Fig.…”

Section: Discussionsupporting

confidence: 85%

Morphological changes of Fusarium oxysporum induced by CF66I, an antifungal compound from Burkholderia cepacia

Lin

et al. 2010

Biotechnol Lett

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The morphological effects of CF66I, an antifungal compound produced by Burkholderia cepacia, on growing hyphae of Fusarium oxysporum were studied by fluorescence microscopy (FM) and transmission electron microscopy (TEM). At 20 μg/ml, CF66I strongly inhibited growth and induced significant changes of the hyphal morphology. These changes included swelling of hyphae with considerable thickening cell wall and abnormal chitin deposition, which was indicative of the alterations in cell wall structure. Furthermore, fluorescein diacetate (FDA) staining indicated the loss of intracellular esterase activity. CF66I probably inhibits fungal growth by interfering with the cell metabolic pathways. At 120 μg/ml, CF66I killed F. oxysporum (accompanied by propidium iodide permeation, intracellular cytoplasm leakage and crushing of hyphal tips), probably by direct damage to the cell membrane. Thus, there are two different antifungal mechanisms of CF66I, depending on its concentration, and further studies on this compound might be useful for us to develop a new class of antifungal agents.

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Section: Discussionsupporting

confidence: 85%

Morphological changes of Fusarium oxysporum induced by CF66I, an antifungal compound from Burkholderia cepacia

Lin

et al. 2010

Biotechnol Lett

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“…In this study, we quantified C. albicans actin mRNA as a means of assessing the viability of C. albicans in a reconstituted-human-skin model of cutaneous candidiasis following the application of the antimycotic. Amorolfine was used because of its effectiveness in the topical therapy of superficial mycoses caused by yeasts and dermatophytes and its high affinity to stratum corneum and nails (12,13).…”

Section: Discussionmentioning

confidence: 99%

Quantification of Candida albicans Actin mRNA by the LightCycler System as a Means of Assessing Viability in a Model of Cutaneous Candidiasis

2001

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The LightCycler system (two-step reverse transcription-PCR-fluorescent hybridization [LC RT-PCR-FH]) was used to quantifyCandida species are frequently found as saprophytes that colonize the surfaces of certain mucous membranes in human. The superficial and disseminated infections caused by them are largely opportunistic, and Candida albicans accounts for most of the etiologic species isolated from cutaneous candidiasis. Microscopic demonstration of yeast elements in potassium hydroxide preparations of infected tissue and cultural isolation of yeast from skin scrapings are used to confirm clinical diagnosis of cutaneous candidiasis. Rapid and highly sensitive diagnostic assays, based on PCR of DNA, have also been used to identify candidal pathogens in tissue (3,4,5,7,8).The amplification of a targeted DNA sequence by PCR does not, however, indicate the viability of the source organism, important information for evaluating the efficacy of a particular therapy and thus for avoiding an unnecessary prolongation of therapy. For this reason, reverse transcription (RT)-PCR of mRNA molecules has been used to assess the viability of microorganisms (9,11,14). However, RT-PCR is a qualitative assay, and this places a limitation on the inferences that can be drawn from the result; differences in the quantities of target mRNAs cannot easily be ascertained from the intensities of amplification bands. A determination of quantitative differences is needed in an assessment of the potencies of different concentrations of an agent or the progressive efficacy of a particular treatment. Furthermore, with the template at low levels, bands are seldom visualized in gel, resulting in falsenegative interpretations (14).In this study, we estimated the viability of C. albicans in a reconstituted-skin model of cutaneous candidiasis by detection of C. albicans ACT mRNA using the LightCycler (LC) system (a two-step RT-PCR-fluorescent hybridization [FH] assay) (10). LC DNA-based identification of fungi has been shown to be highly sensitive and rapid (10), and we applied of this system to rapidly quantify C. albicans ACT mRNA. The ACT mRNA was specifically targeted because of the constitutive nature of the gene and the major role of actin in cell division (6,15).

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Antifungal therapy - state of the art at the beginning of the 21st century

Polak¹

2003

Antifungal Agents

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The effects of amorolfine and oxiconazole on the ultrastructure of Trichophyton mentagrophytes. A comparison

Cited by 12 publications

References 8 publications

Morphological changes of Fusarium oxysporum induced by CF66I, an antifungal compound from Burkholderia cepacia

Morphological changes of Fusarium oxysporum induced by CF66I, an antifungal compound from Burkholderia cepacia

Quantification of Candida albicans Actin mRNA by the LightCycler System as a Means of Assessing Viability in a Model of Cutaneous Candidiasis

Antifungal therapy - state of the art at the beginning of the 21st century

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