The ultrastructure of human secretory ameloblasts

Matthiessen, M. E.; Bülow, F. A. von

doi:10.1007/bf00335730

Cited by 27 publications

(9 citation statements)

References 8 publications

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“…Some authors fixed by immersion in glutaraldehyde or glutaraldehyde-formaldehyde mixture (Garant and Nalbandian, 1968;Matthiessen and Bulow, 1969;Lester, 1970;Katchburian and Holt, 1972;Decker, 1973;Slavkin et al, 1976). Only a few studies used teeth fixed by whole body perfusion (Jessen, 1968;Kallenbach, 1971Kallenbach, , 1973Kallenbach, , 1976Kallenbach, , 1977.…”

Section: Discussionmentioning

confidence: 97%

Relationship between the quality of fixation and the presence of stippled material in newly formed enamel of the rat incisor

Nanci

Warshawsky

1984

Anat. Rec.

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Extracellular accumulation of a granular material that is presumed to be an organic "precursor" to mineralized enamel has been reported. This material, generally referred to as ""stippled material," was observed mainly after immersion fixation with osmium tetroxide. In studies with perfusion fixation, the presence of stippled material was inconsistent. Therefore, it appeared that the occurrence of stippled material was dependent on the method of fixation. To test this assumption, tissues were fixed by immersion in either osmium tetroxide or glutaraldehyde and by perfusion with either glutaraldehyde or a mixture of acrolein, glutaraldehyde, and formaldehyde. It was found that as the quality of cellular preservation improved, the occurrence of stippled material decreased. Since no stippled material could be found in material judged to be well fixed, it was concluded that stippled material is not an extracellular precursor to mineralized enamel, but is a breakdown product resulting from poor fixation.

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Section: Discussionmentioning

confidence: 97%

Relationship between the quality of fixation and the presence of stippled material in newly formed enamel of the rat incisor

Nanci

Warshawsky

1984

Anat. Rec.

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“…The first is via classical exocytosis, whereby the membrane of secretory granules fuses with surface cell membrane (Frank and Nalbandian, 1967;Reith, 1967;Matthiessen and Bulow, 1969;Frank, 1979) or fuses to deep membrane infoldings (Kallenbach, 1977;Simmelink, 1982). The second involves a tubular network interconnecting many granules (Smith, 1979), thereby making the simultaneous release of the content of many granules possible.…”

Section: Relationship Between Growth Sites and Enamel Organizationmentioning

confidence: 99%

“…Although the relationship between growth areas and Tomes' process has not been well established, it is known that both rod and interrod growth sites are associated with infolded membrane (Warshawsky et al, 1981), and secretory granules often accumulate adjacent to these infoldings (Weinstock and Leblond, 1971). Granules fused to surface membranes have occasionally been observed at the rod growth site (Reith, 1967;Frank and Nalbandian, 1967;Matthiessen and Bulow, 1969;Frank, 1979) but not at the interrod growth sites where few secretory granules have been observed (Weinstock and Leblond, 1971;Kallenbach, 1973). Alternatively, it has been postulated that secretory granules fuse to an internal tubular network (Smith, 1979) or to deep membrane invaginations (Kallenbach, 1977;Simmelink, 1982).…”

mentioning

confidence: 99%

Characterization of putative secretory sites on ameloblasts of the rat incisor

Nanci

Warshawsky

1984

Am. J. Anat.

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The distribution and structure of the putative sites where enamel matrix is secreted from the ameloblast were studied by correlating the external topography with the distribution of organelles in Tomes' process cut in various planes of section. Both the interrod and rod secretion sites are associated with deep membrane infoldings. It was found that the interrod secretion site completely surrounds each ameloblast, and the marked interdigitation of adjacent cells results in a cooperative growth front for interrod enamel. In contrast, the rod secretion site is present on only one surface of the interdigitating portion of Tomes' process. Numerous granules were observed adjacent to the membrane infoldings associated with both sites, and granules were seen fused to membrane infoldings suggesting that the matrix of enamel is a merocrine secretion product.

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“…One is the material located between the distal ends of the secretory ameloblasts and the mineralization front (36). It has been observed in this location in developing enamel of many different species (2,4,5,8,15,16,17,21,34,39) and also in enamel formed in vitro (37). It is generally described as a stippled or finely granular material.…”

mentioning

confidence: 97%

“…In fully mature rat and human enamel, the crystals have filled in most of the space originally available between the thin ribbonshaped crystals. Only a narrow space, [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] A in width, separates individual crystals (20,26).…”

mentioning

confidence: 99%

Matrix-Mineral Relationships-A. Morphologist's Viewpoint

Nylen

1979

J Dent Res

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The literature on the ultrastructural morphology of the enamel matrix and its relationship to the crystals is reviewed. Two morphological entities of the matrix are discussed. One is the so-called stippled material which may be the initial cell product; the other, variously described as fibrillar, lamellar, tubular or helical, is thought by many to play a crucial role in nucleation and orientation of the crystals. A number of observations, however, suggest that the latter structures form secondarily to the crystals and that in reality they represent organic material adsorbed to the crystal surface and maintained as independent structures upon removal of the mineral. The need for additional studies is stressed including systematic studies of interactions between constituents of the organic matrix and the apatite crystals.

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The ultrastructure of human secretory ameloblasts

Cited by 27 publications

References 8 publications

Relationship between the quality of fixation and the presence of stippled material in newly formed enamel of the rat incisor

Relationship between the quality of fixation and the presence of stippled material in newly formed enamel of the rat incisor

Characterization of putative secretory sites on ameloblasts of the rat incisor

Matrix-Mineral Relationships-A. Morphologist's Viewpoint

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