Matrix-Mineral Relationships-A. Morphologist's Viewpoint

Nylen, Marie U.

doi:10.1177/00220345790580024601

Cited by 47 publications

(31 citation statements)

References 27 publications

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“…Although the correlation between mineral development, matrix breakdown, and selective protein loss suggests a functional participation of the matrix proteins in the mechanism of enamel mineralization (Eastoe, 1979;Fearnhead, 1979;Glimcher, 1979;Nylen, 1979;Fincham, 1983), the precise role of the enamel proteins is still unknown. In an effort to understand this process, we investigated the effects of purified enamel proteins on the crystal growth of isolated enamel apatite in vitro.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Seeded Growth of Enamel Apatite Crystals by Amelogenin and Enamelin Proteins in vitro

et al. 1984

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The effect of enamel matrix proteins on the seeded growth of enamel apatite crystals was studied in stable supersaturated solutions at pH 7.4 and 37 degrees C. Of the two major protein classes in the enamel matrix, the enamelins were considerably more effective than the amelogenins in retarding seeded growth. However, the amelogenin species that did show significant inhibitory activity are those known to be lost first from the enamel matrix during the rapid mineralization stage of enamel maturation.

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Section: Introductionmentioning

confidence: 99%

Inhibition of Seeded Growth of Enamel Apatite Crystals by Amelogenin and Enamelin Proteins in vitro

et al. 1984

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“…Yet, little work has been performed on young enamel without the interference of heavy metal fixatives or stains such as osmium tetroxide, uranyl acetate, and lead citrate. Nylen (1979) and Leblond and Warshawsky (1979) suggested that the organic matrix of 0003-276X/8212022-0177$03.00 0 1982 Alan R. Liss, Inc.…”

mentioning

confidence: 99%

Electron microscopic studies on the potential loss of crystallites from routinely processed sections of young enamel in the rat incisor

Bishop

Warshawsky

1982

Anat. Rec.

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Newly formed rat incisor enamel was fixed aqueously by perfusion with glutaraldehyde and anhydrously by immersion in ethylene glycol. Ultrathin sections were studied using transmission electron microscopy and electron diffraction. Aqueously processed enamel was shown to lose its mineral content when sectioned on distilled water. This mineral loss was minimized by limiting the exposure of sections to the water. In such preparations, enamel crystallites were seen by virtue of their intrinsic electron density only. Selected area electron diffraction provided corroborative evidence for the presence or absence of crystallites in the sections. Observations on mineralized sections and on stained mineralized and distilled-water-demineralized sections revealed organic material apparently in the same location as the crystallites. Anhydrously processed enamel which was sectioned on ethylene glycol showed a similar appearance of the crystallites. This appearance was not obviously altered after staining despite evidence that organelles in the ameloblasts were stained. In view of the observations that both methods yielded similar crystallite morphology, it was concluded that aqueous techniques can be used to study the relationship between organic and inorganic components. However, valid description of crystallites in such preparations requires minimal exposure of ultrathin sections to water.

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“…The specific surface area of the mineral in secretary enamel was much greater than that in the maturation enamel (Table 1). This is not surprising, because the crystallites in secretorystage enamel are much smaller than those in maturation-stage enamel (Nylen, 1979;Daculsi et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

“…However, the greater weight percentage of fluoride in the secretary stage may also be explained if the specific surface area of the crystallites was greater in the secretory-stage enamel than in the maturation-stage enamel. The smaller crystallite size in the secretary stage (Nylen, 1979;Daculsi et al, 1984) would mean that a greater proportion of the lattice ions would be on the surfaces of the crystallites for reaction with fluoride entering the enamel. The specific surface areas of developing enamel and fluoride uptake by developing enamel on a surface-area basis have not been reported.…”

Section: Introductionmentioning

confidence: 99%

The in vitro Uptake of Fluoride by Secretory and Maturation Stage Bovine Enamel

et al. 1988

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The objectives of this study were to determine the specific surface area of secretory-stage and of maturation-stage enamel, to compare the fluoride uptake by isolated enamel at these two stages on a surface-area basis, and to examine the effect of the organic matrix on the fluoride uptake by whole enamel. Fetal bovine secretory and maturation stage enamel samples were collected, and a portion of the enamel at each developmental stage was treated with hydrazine for removal of the organic matrix. The specific surface areas of the enamel mineral, as determined by the multi-point BET method, were 59.3 m2/g in the secretory stage and 37.9 m2/g in the maturation stage. Whole and deproteinated enamel samples were equilibrated in buffered solutions containing 10(-5) to 10(-3) mol/L fluoride, and the uptake was measured with a fluoride specific electrode. The results indicate that the in vitro fluoride uptake was controlled solely by the surface area of the apatitic mineral and that the organic matrix did not contribute to the fluoride uptake.

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Matrix-Mineral Relationships-A. Morphologist's Viewpoint

Cited by 47 publications

References 27 publications

Inhibition of Seeded Growth of Enamel Apatite Crystals by Amelogenin and Enamelin Proteins in vitro

Inhibition of Seeded Growth of Enamel Apatite Crystals by Amelogenin and Enamelin Proteins in vitro

Electron microscopic studies on the potential loss of crystallites from routinely processed sections of young enamel in the rat incisor

The in vitro Uptake of Fluoride by Secretory and Maturation Stage Bovine Enamel

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