The ultrastructure of hepatic endothelial cells in the correction of steatosis with new biologically active compound Angiolin

Півторак, В. І.; Mazur, I. A.; Voloshyn, M. A.

doi:10.14739/2310-1237.2015.3.55587

Cited by 4 publications

(1 citation statement)

References 2 publications

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“…We have studied the endothelial protective effect of the compound Angiolin. Our studies [23] of the submicroscopic state of animal liver endothelial cells in the correction of NASH by angiolin have established that the ultrastructural organization of endothelial cells has a typical long elongated shape. Elliptical nuclei include a karyoplasm made of euchromatin, which is confined to a karyolemma with clear nuclear membranes.…”

Section: Resultsmentioning

confidence: 73%

Features of the structural and functional parameters of the liver in experimental steatohepatitis and its correction in obese rats

Півторак

2019

Rep. of Morph.

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The improvement of the pharmacological strategy of non-alcoholic fatty liver disease is based on the study of the effect of pharmaceutical preparations on the structure and function of the liver. The pathogenesis of steatohepatitis is complex and multifactorial, mainly involving genetic, metabolic and environmental factors. The purpose of the study was to characterize the structural and functional parameters of the liver when using the biologically active compound Angiolin for the correction of experimental steatohepatitis. An experimental study was performed on 110 sexually mature white male rats weighing 180-220 grams, which were kept on a standard diet of vivarium. All animals were divided into two groups: control (30 intact animals) and experimental (80 animals). A model of non-alcoholic steatohepatitis was created for all animals of the experimental group. They were kept on a hypercaloric diet with a high fat and high cholesterol content for 8 weeks. After that, part of the animals (10 rats) was withdrawn from the experiment by intrapleural administration of sodium thiopental (50 mg/kg) and the necessary biochemical and morphological studies were performed. Part of the animals (30 rats) was continued to be kept on a high-fat diet for 4 weeks and the biologically active compound Angiolin was administered (20 rats), and Rings-Locke solutions were administered to 10 rats. After the creation of the model, the other animals of the experimental group (40 rats) were transferred to a full-fledged standard semi-synthetic starch-casein diet, and the biologically active compound Angiolin was administered for 20 rats and Ringer-Locke solution for another 20 rats for 4 weeks. Macroscopic evaluation and description of the liver of animals was carried out after withdrawal under thiopental anesthesia. Statistical analysis of the results was carried out using the program “STATISTICA 8” using parametric and non-parametric methods for assessing the results. It was found that the use of the biologically active compound Angiolin once a day for 30 days can reduce cytolysis syndrome (reduce biochemical parameters such as ALT, AST, gamma-glutamyl transpeptidase), reduce cholestasis syndrome (decrease in alkaline phosphatase level), and normalize liver function, improves the morphological state of hepatocytes, which indicates the normalization of the structural and functional state of the liver.

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Section: Resultsmentioning

confidence: 73%

Features of the structural and functional parameters of the liver in experimental steatohepatitis and its correction in obese rats

Півторак

2019

Rep. of Morph.

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Comparative Assessment of the Effectiveness of HSP70 / HIF-1α System Modulators after Prenatal Hypoxia

Aliyeva,

Belenichev,

Bukhtiyarova

et al. 2024

Biomed. Pharmacol. J.

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Prenatal hypoxia (PH) poses a significant threat to fetal development and may be responsible for neonatal mortality or neurodevelopmental abnormalities. The proteins HSP70 and HIF-1, which hold a distinct significance in the cellular reaction to PH, can be regarded as potential targets for pharmaceutical interventions aimed at mitigating the repercussions of chronic PH. This study aimed to identify a possible correlation between offspring survival and stages of expression of endogenous neuroprotective factors (HSP70 and HIF-1) after chronic prenatal hypoxia with course administration of potential HSP70 modulators (angiolin, piracetam, thiotriazoline, nicomex, cerebrocurin, tamoxifen, L-arginine, glutoredoxin, HSF-1, and mildronate). In the rat offspring after PH we determined the plasma concentrations of HSP70 and HIF-1 by solid-phase ELISA immunoassay, and the expression of HIF-1 mRNA and HSP70 mRNA by real-time PCR. For the first time, we found a positive correlation between offspring survival after PH and the expression of HIF-1 and HSP70, both in groups without experimental therapy and in groups receiving pharmacological agents. The course administration of HSP70/HIF-1α modulators, especially angiolin (50 mg/kg), cerebrocurin (150 mg/kg), and HSF-1 (50 mg/kg), to rats that underwent PH reduces postnatal lethality, increases blood plasma concentrations of HSP70 and HIF-1α, and positively affects the expression level of HIF-1α mRNA in the rat brain. These drugs can be considered as the most promising drug candidates for new therapeutic strategies of pharmacological correction of the consequences of chronic PH.

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Efficacy of an Experimental Model of Non-Alcoholic Fatty Liver Disease Based on a High-Fat Diet With Cholesterol

Pivtorak¹,

Sydorenko²,

Monastyrskyi³

et al. 2022

WOMAB

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The ultrastructure of hepatic endothelial cells in the correction of steatosis with new biologically active compound Angiolin

Cited by 4 publications

References 2 publications

Features of the structural and functional parameters of the liver in experimental steatohepatitis and its correction in obese rats

Features of the structural and functional parameters of the liver in experimental steatohepatitis and its correction in obese rats

Comparative Assessment of the Effectiveness of HSP70 / HIF-1α System Modulators after Prenatal Hypoxia

Efficacy of an Experimental Model of Non-Alcoholic Fatty Liver Disease Based on a High-Fat Diet With Cholesterol

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