The Ultrastructure and Histology of Hepatocellular Carcinomas of English Sole (Parophrys vetulus) from Puget Sound, Washington

Stehr, Carla M.; Rhodes, Linda D.; Myers, Mark S.

doi:10.1177/019262338801600402

Cited by 14 publications

(7 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These authors identified the fibrils as dilated rough endoplasmic reticulum (RER) containing microfilamentous material. Unlike Stehr et al (1988), h o w ever, we found these structures exclusively in nontumorous cells, even in the case of one llver which contained a carcinoma.…”

Section: Focl Of Cellular Alteration ] Hydropic Vacljoliution Of Bilicontrasting

confidence: 55%

See 1 more Smart Citation

Diseases of flounder Platichthys flesus in Dutch coastal and estuarine waters, with particular reference to environmental stress factors. II. Liver histopathology

Ad¹,

Pw²

1996

Dis. Aquat. Org.

126

View full text Add to dashboard Cite

Diseases of flounder Platichthys flesus in Dutchcoastal and estuarine waters, with particular reference to environmental stress factors. were diagnosed as hepatocellular carcinoma. Most of the remaining nodules were diagnosed as foci of cellular alteration, whlch are considered to be preneoplastic lesions. Routine histopathological examination of livers showing no gross pathology resulted in the identification of a range of lesions including hepatocellular adenoma, foci of cellular alteration, hydropic vacuolization of biliary epithelial cells and hepatocytes, inflammatory lesions, focal necrosis, regenerative foci, and fibrillar hepatocytes of unknown significance. In addition, indices were used to quantify the presence of storage vacuoles (glycogen and lipid) and the relative density of melanomacrophage centres. The results indicate that a small proportion of neoplasms would be missed if only livers with grossly detectable nodules were subjected to histological examination. The prevalence of foci of cellular alteration at the different sampling sites showed a good correspondence with that of neoplasms, providing support for the hypothesis that these conditions represent stages of the same process. Of the other lesions and quantitative ~ndices, only hydropic vacuolization of billary epithelia] cells had a spatial distribution similar to that of neoplasms. 11 is concluded that liver neoplasms and especially their precursor lesions in flounder are promising tools for monitoring exposure to potential carcinogens, provided that migration patterns are explicitly taken into account. Furthermore, experimental studies are needed to investigate the chemical agents responsible for the onset of these early lesions and their progression into neoplasms, and also to evaluate the contribution of other environmental and host-related factors. Liver histopathology

show abstract

Section: Focl Of Cellular Alteration ] Hydropic Vacljoliution Of Bilicontrasting

confidence: 55%

“…Using light microscopy, the resulting structures resembled the fibrillar hepatocytes described by Stehr et al (1988) in hepatocellular carcinomas of English sole. A similar finding was reported by Kohler (1989) in non-tumourous liver in European flounder.…”

Section: Focl Of Cellular Alteration ] Hydropic Vacljoliution Of Bilimentioning

confidence: 67%

Diseases of flounder Platichthys flesus in Dutch coastal and estuarine waters, with particular reference to environmental stress factors. II. Liver histopathology

Ad¹,

Pw²

1996

Dis. Aquat. Org.

126

View full text Add to dashboard Cite

show abstract

“…It is difficult to classify species absolutely since many factors affect the amount of storage material within the hepatocytes. Differences in types of storage material may even exist among specimens of the same species, as has been demonstrated in English sole (Pleuronectes vetulus) (Stehr et al 1988).…”

Section: Discussionmentioning

confidence: 91%

“…In sexually mature flatfish species, such as European flounder (Platichthys flesus), winter flounder (Pseudopleuronectes americanus), and plaice (Pleuronectes platessa), the pancreatic tissue is more scattered throughout the liver (Timashova 1982;Ko¨hler 1990;Murchelano and Wolke 1991;March and Reisman 1995;Vethaak and Wester 1996). As in other fish, the histomorphology of the liver in sexually immature L. ferruginea causes difficulties in determining the functional liver units and in explaining the appearance of histopathological changes (Hampton et al 1985(Hampton et al , 1988Morrison 1987;Stehr et al 1988;Ko¨hler 1989;Krause and Cutts 1994;Vethaak and Wester 1996). In vivo microscopy of liver microvasculature in rainbow trout revealed a repeating pattern of alternation between afferent and efferent regions (Hinton and Lauren 1989).…”

Section: Discussionmentioning

confidence: 99%

“…The histomorphology of liver of flatfish such as winter flounder (Pseudopleuronectes americanus), European flounder (Platichthys flesus), English sole (Pleuronectes vetulus), and dab (Limanda limanda) resembles that of other teleosts (Hampton et al 1985(Hampton et al , 1988Morrison 1987;Stehr et al 1988;Ko¨hler 1989;Vethaak and Wester 1996, and references therein). The basic morphology and physiology of the liver of yellowtail flounder have not been studied to date, and sex differences in the studied species, and the potential differences between wild and cultured fish have also received little attention.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structure of and energy reserves in the liver of wild and cultured yellowtail flounder, Limanda ferruginea

Ree

Spurrell

2003

Marine Biology

View full text Add to dashboard Cite

Knowledge of the structure and energy reserves, in the liver of commercially important fish species, is important in understanding metabolic processes and in assessing the impact of potential environmental physical and chemical stressors in both wild and cultured stocks. The present study investigated the microscopic morphology and histochemistry (total and neutral lipids, glycogen) of liver tissue of wild (3 + ) and cultured (1 + ) sexually immature female and male yellowtail flounder (Limanda ferruginea Storer), sampled in late April 2001. Hepatosomatic indices [HSI: (liver weight/body weight)liver weight)·100] of cultured fish were significantly higher than those of wild fish. Females in the cultured group had significantly lower HSIs than males. The liver of both wild and cultured L. ferruginea was interspersed with pancreatic tissue. The main components of the liver tissue were irregular cords of hepatocytes arranged in tubules which surrounded vascular sinusoids. The hepatocytes contained an abundance of lipid, much of which appeared to be neutral lipids, in both sexes of the cultured fish. Total and neutral lipid droplets were larger, and the area occupied by these droplets was significantly greater in both cultured females and males compared to the wild fish, suggesting lipidosis in the cultured fish. In the cultured fish these differences were sex-dependent, the females having significantly more total and neutral lipids in hepatocytes than the males. This suggests a potentially greater storage capacity in females and/or a higher lipid metabolism in males. There were no statistically significant differences in glycogen content between the cultured and wild fish, or between the sexes in both sampling groups.

show abstract

Effect of Toxaphene on Isolated Hepatocytes of the Yellowtail Flounder, Pleuronectes ferrugineus Storer

Ree

Spurrell

2000

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

The Ultrastructure and Histology of Hepatocellular Carcinomas of English Sole (Parophrys vetulus) from Puget Sound, Washington

Abstract: ABSTRACT

Cited by 14 publications

References 41 publications

Diseases of flounder Platichthys flesus in Dutch coastal and estuarine waters, with particular reference to environmental stress factors. II. Liver histopathology

Diseases of flounder Platichthys flesus in Dutch coastal and estuarine waters, with particular reference to environmental stress factors. II. Liver histopathology

Structure of and energy reserves in the liver of wild and cultured yellowtail flounder, Limanda ferruginea

Effect of Toxaphene on Isolated Hepatocytes of the Yellowtail Flounder, Pleuronectes ferrugineus Storer

Contact Info

Product

Resources

About