The Ultrastructure and Functions of the Silk Gland Cells of Bombyx mori

Akai, Hiromu

doi:10.1007/978-1-4613-2715-8_9

Cited by 41 publications

(35 citation statements)

References 97 publications

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“…This same characteristic was described during the degenerative process of the larval salivary glands of Diatraea saccharalis (Victoriano, 2002), M. sexta (Lockshin and Zakeri, 1994), Calliphora vomitoria (Bowen et al, 1993), and Calliphora erythrocephala (Levy and Bautz, 1985). In the silk gland of Bombyx mori, Akai (1984) observed that only after the degeneration of the cytoplasm, did the nucleus exhibit an altered morphology. This same result was registered by Senhal et al (1983) in silk glands of Galleria mellonella.…”

Section: Discussionmentioning

confidence: 51%

Nuclear alterations associated to programmed cell death in larval salivary glands of Apis mellifera (Hymenoptera: Apidae)

Silva-Zacarín

Taboga

Moraes

2008

Micron

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Section: Discussionmentioning

confidence: 51%

Nuclear alterations associated to programmed cell death in larval salivary glands of Apis mellifera (Hymenoptera: Apidae)

Silva-Zacarín

Taboga

Moraes

2008

Micron

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“…the presence of lipid droplets in the basal and supranuclear cytoplasm, corresponding to the positive to histochemically correspond to the lipid storied as reserve in animal and secretory vesicles at the apical cytoplasm, these secretory (Victoriano et al 2003), similar to those described for other Lepidoptera (Willey & Lai-Fook 1974, Akai 1984, Sehnal & Akai 1990). …”

Section: Resultsmentioning

confidence: 61%

Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae)

Victoriano¹,

Pinheiro²,

Gregório³

2007

Neotrop. entomol.

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Neotropical Entomology 36(5): 707-711 (2007) Evidências Histoquímicas e Ultra-Estruturais de Secreção Lipídica pela Glândula da Seda da Broca da Canade-Açúcar, Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae)RESUMO -A glândula da seda de larvas de Lepidoptera é responsável pela produção da seda usada na secreção de seda de insetos, embora a presença desse componente contribua para a resistência da seda secreção da glândula da seda de larvas de Diatraea saccharalis (Fabricius), bem como caracterizar a região glandular responsável pela secreção. A glândula da seda foi submetida a procedimentos histoquímicos para detecção de lipídeos e convencionalmente preparada para análise ultra-estrutural. Gotas lipídicas foram histoquimicamente detectadas no citoplasma apical de células da região anterior e no lúmen, entre os microvilos. A análise ultra-estrutural da região anterior mostrou material lipídico, visualizado como com sericina; material semelhante foi observado dentro do lúmen, adjacente aos microvilos. Nenhum componente secretor lipídico foi detectado nas células ou no lúmen da região posterior. Os resultados sugerem que a seda produzida pela D. saccharalis tem, pelo menos, um discreto componente lipídico, que é secretado pela região anterior, junto com a sericina.PALAVRAS-CHAVE: Célula secretora, inseto, larva, lipídio ABSTRACT -The silk gland in Lepidoptera larvae is responsible for the silk production used for shelter characterizes the glandular region and detects the presence of lipid components in the secretion of the silk gland of Diatraea saccharalis detected in both the apical cytoplasm of cell of the anterior region and in the lumen among the microvilli.neither in the lumen of the posterior region. Our results suggest that the silk produced by D. saccharalis

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“…The anterior silk gland is a thin duct composed of about 300 cells and the surface facing the lumen is lined by the thick cuticular intima. Electron microscopic examination showed a layer of electron-lucent granules in the peripheral cytoplasm of anterior silk gland cells with finely ramified thin tubules extending into the plasma membrane facing the lumen, forming a layer of approximately 4 pm thickness (1). The monoclonal antibody against the hemolymph 350 kDa lectin strongly stained the peripheral thin layer of cytoplasm as well as the cuticular intima.…”

Section: Discussionmentioning

confidence: 99%

“…This lectin may be synthesized in the cells or be formed by processing of the hemolymph 350 kDa lectin. The structures in the peripheral layer are considered to produce fibers that may be precursors of the cuticular intima (1 ). Electron microscopic observations suggest that the fibers may aggregate to form the dense-body immediately inside the plasma membrane.…”

Section: Discussionmentioning

confidence: 99%

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Occurrence of Lectin in the Silkgland of the Silkworm, Bombyx mori

et al. 1991

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Monoclonal antibodies were prepared against the 350 kDa lectin purified from larval hemolymph of the silkworm, Bombyx mori. The antibodies inhibited the hemagglutinating activity (HA activity) and bound 'specifically to the hemolymph 350 kDa lectin on Western blotting analysis. lmmunohistological observations revealed the occurrence of lectin in the cuticular intima of the anterior silk gland, but not the middle or posterior silk glands of fifth instar larvae of Bombyx mori. Extracts from the anterior silk glands showed HA activity and exhibited the same biochemical characteristics as those of the 350 kDa lectin in the hemolymph. These results suggested that lectin-like molecules in epithelial tissues may be important in histolysis during molting and metamorphosis.

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The Ultrastructure and Functions of the Silk Gland Cells of Bombyx mori

Cited by 41 publications

References 97 publications

Nuclear alterations associated to programmed cell death in larval salivary glands of Apis mellifera (Hymenoptera: Apidae)

Nuclear alterations associated to programmed cell death in larval salivary glands of Apis mellifera (Hymenoptera: Apidae)

Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae)

Occurrence of Lectin in the Silkgland of the Silkworm, Bombyx mori

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