The UDP-glucosyltransferase multigene family in Bombyx mori

Huang, Feifei; Chai, Chun-Li; Zhang, Ze; Zeng-hu, Liu; Dai, Fangyin; Lü, Cheng; Zhang, Xiang

doi:10.1186/1471-2164-9-563

Cited by 102 publications

(91 citation statements)

References 38 publications

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“…It is, however, regarded as an important component of metabolic detoxification in insects, playing crucial roles in cuticular tanning (Hopkins and Kramer, 1992), pigmentation (Hopkins and Ahmad, 1991;Wiesen et al, 1994;Mizokami and Yoshitama, 2009), as well as detoxification. UGTs are encoded by a multigene family, which has been recently analyzed in several insects from which genomic information is available (Luque and O'Reilly, 2002;Huang et al, 2008). Further studies are necessary not only to determine which gene(s) is involved in the glucosylation of capsaicin but also to understand its regulatory mechanism, in relation to the host-plant adaptation of herbivores.…”

Section: Perspectivesmentioning

confidence: 99%

Metabolic detoxification of capsaicin by UDP‐glycosyltransferase in three Helicoverpa species

Ahn

Badenes‐Pérez

Reichelt

et al. 2011

Arch Insect Biochem Physiol

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Capsaicin β-glucoside was isolated from the feces of Helicoverpa armigera, Helicoverpa assulta, and Helicoverpa zea that fed on capsaicin-supplemented artificial diet. The chemical structure was identified by NMR spectroscopic analysis as well as by enzymatic hydrolysis. The excretion rates of the glucoside were different among the three species; those in the two generalists, H. armigera and H. zea, were higher than in a specialist, H. assulta. UDP-glycosyltransferases (UGT) enzyme activities measured from the whole larval homogenate of the three species with capsaicin and UDP-glucose as substrates were also higher in the two generalists. Compared among five different larval tissues (labial glands, testes from male larvae, midgut, the Malpighian tubules (MT), and fat body) from the three species, the formation of the capsaicin glucoside by one or more UGT is high in the fat body of all the three species as expected, as well as in H. assulta MT. Optimization of the enzyme assay method is also described in detail. Although the lower excretion rate of the unaltered capsaicin in H. assulta indicates higher metabolic capacity toward capsacin than in the other two generalists, the glucosylation per se seems to be insufficient to explain the decrease in capsaicin in the specialist, suggesting that H. assulta might have another important mechanism to deal with capsaicin more specifically.

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Section: Perspectivesmentioning

confidence: 99%

Metabolic detoxification of capsaicin by UDP‐glycosyltransferase in three Helicoverpa species

Ahn

Badenes‐Pérez

Reichelt

et al. 2011

Arch Insect Biochem Physiol

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“…We speculate that venom allergen 5 is important for cell growth and development. This gene may be a multifunctional protein, performing different functions at different stages, as do the insect UDP-glycosyltransferases that play important roles in detoxication of xenobiotics, cuticle formation, pigmentation, and olfaction (Huang et al 2008, Bozzolan et al 2014. The specific biological function of venom allergen 5 in Cx.…”

Section: Discussionmentioning

confidence: 99%

Venom allergen 5is Associated With Deltamethrin Resistance inCulex pipiens pallens(Diptera: Culicidae)

Lei

Hong

et al. 2015

J Med Entomol

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The mosquito, Culex pipiens pallens (L.), is an important vector of encephalitis and filariasis in northern China. The control of these mosquitoes occurs primarily via the use of pyrethroid insecticides, such as deltamethrin. The widespread and improper application of pyrethroid has resulted in the evolution of pyrethroid resistance amongst many mosquito populations, including Cx. pipiens pallens. Previous studies using high-throughput transcriptome sequencing have identified that the venom allergen 5 gene is differentially expressed between deltamethrin-susceptible and deltamethrin-resistant Cx. pipiens pallens. In this study, quantitative real-time polymerase chain reaction analyses revealed that venom allergen 5 was significantly overexpressed in adult females of both deltamethrin-resistant laboratory populations and two field populations. The transcriptional level of venom allergen 5 in the laboratory populations was elevated as the levels of deltamethrin resistance increased. Full-length cDNAs of the venom allergen 5 gene were cloned from Cx. pipiens pallens, and contained an open reading frame of 765 bp, encoding a protein with 254 amino acids. The deduced amino acid sequence shared 100% identity with the ortholog in Culex quinquefasciatus Say. The overexpression of venom allergen 5 decreased the susceptibility of mosquito cells to deltamethrin, while knockdown of this gene by RNAi increased the susceptibility of mosquitoes to deltamethrin. This study provides the first evidence of the association between the venom allergen 5 gene and deltamethrin resistance in mosquitoes.

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“…The B. mori UGT sequences were collected from KAIKObase (Shimomura et al, 2009) and SilkDB (Duan et al, 2010) based on the gene names in a previous report (Huang et al, 2008), which however did not give the actual sequences. BLAST searches of unannotated genomic contigs in NCBI were also performed to obtain additional sequences.…”

Section: Blast Searches Of Insect Ugt Sequences From Public Databasesmentioning

confidence: 99%

“…The molecular identities of the insect UGTs are, however, relatively unknown compared to the other detoxification gene families in insects or to the vertebrate UGT gene families. Although the UGT gene families in D. melanogaster and in B. mori (Huang et al, 2008) have been reported, they have not been compared in the context of the diversity of other insects.…”

Section: Introductionmentioning

confidence: 97%