The UDP-glucose: glycoprotein glucosyltransferase (UGGT), a key enzyme in ER quality control, plays a significant role in plant growth as well as biotic and abiotic stress in Arabidopsis thaliana

Blanco‐Herrera, Francisca; Moreno, Adrián A.; Tapia, Rodrigo; Reyes, Francisca; Araya, Macarena; D’Alessio, Cecilia; Parodi, Armando J.; Orellana, Ariel

doi:10.1186/s12870-015-0525-2

Cited by 64 publications

(55 citation statements)

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“…The enzyme is a key player in the quality control mechanism for newly synthesized glycoproteins in the endoplasmic reticulum (ER). This organelle hosts the synthesis/folding of proteins secreted extracellularly or delivered to endomembrane system [74]. Quality control includes the calnexin (CNX)/calreticulin (CRT) cycle, which involves lectin-chaperones retaining N-glycosylated proteins at the ER while they undergo the folding process.…”

Section: Resultsmentioning

confidence: 99%

Analysis of transcriptional response to heat stress in Rhazya stricta

et al. 2016

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BackgroundClimate change is predicted to be a serious threat to agriculture due to the need for crops to be able to tolerate increased heat stress. Desert plants have already adapted to high levels of heat stress so they make excellent systems for identifying genes involved in thermotolerance. Rhazya stricta is an evergreen shrub that is native to extremely hot regions across Western and South Asia, making it an excellent system for examining plant responses to heat stress. Transcriptomes of apical and mature leaves of R. stricta were analyzed at different temperatures during several time points of the day to detect heat response mechanisms that might confer thermotolerance and protection of the plant photosynthetic apparatus.ResultsBiological pathways that were crosstalking during the day involved the biosynthesis of several heat stress-related compounds, including soluble sugars, polyols, secondary metabolites, phenolics and methionine. Highly downregulated leaf transcripts at the hottest time of the day (40–42.4 °C) included genes encoding cyclin, cytochrome p450/secologanin synthase and U-box containing proteins, while upregulated, abundant transcripts included genes encoding heat shock proteins (HSPs), chaperones, UDP-glycosyltransferase, aquaporins and protein transparent testa 12. The upregulation of transcripts encoding HSPs, chaperones and UDP-glucosyltransferase and downregulation of transcripts encoding U-box containing proteins likely contributed to thermotolerance in R. stricta leaf by correcting protein folding and preventing protein degradation. Transcription factors that may regulate expression of genes encoding HSPs and chaperones under heat stress included HSFA2 to 4, AP2-EREBP and WRKY27.ConclusionThis study contributed new insights into the regulatory mechanisms of thermotolerance in the wild plant species R. stricta, an arid land, perennial evergreen shrub common in the Arabian Peninsula and Indian subcontinent. Enzymes from several pathways are interacting in the biosynthesis of soluble sugars, polyols, secondary metabolites, phenolics and methionine and are the primary contributors to thermotolerance in this species.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0938-6) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Analysis of transcriptional response to heat stress in Rhazya stricta

et al. 2016

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“…DNA replication and cell growth associated genes with significant SNPs included Glyma.01G193400 (AtCYCD5), Glyma.02G074800 (AtGAI), and Glyma.05G168600 (AtEBS1), all important components regulating plant growth and the cell cycle [73][74][75]. In addition, significant SNPs were also identified in two sucrose transporter (AtSUC2) homologs (Glyma.02 g075000 and Glyma.02 g075100).…”

Section: Gwas Identifies Novel Idc Genes Throughout the Soybean Genomementioning

confidence: 99%

Deconstructing the genetic architecture of iron deficiency chlorosis in soybean using genome-wide approaches

et al. 2020

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Background: Iron (Fe) is an essential micronutrient for plant growth and development. Iron deficiency chlorosis (IDC), caused by calcareous soils or high soil pH, can limit iron availability, negatively affecting soybean (Glycine max) yield. This study leverages genome-wide association study (GWAS) and a genome-wide epistatic study (GWES) with previous gene expression studies to identify regions of the soybean genome important in iron deficiency tolerance. Results: A GWAS and a GWES were performed using 460 diverse soybean PI lines from 27 countries, in field and hydroponic iron stress conditions, using more than 36,000 single nucleotide polymorphism (SNP) markers. Combining this approach with available RNA-sequencing data identified significant markers, genomic regions, and novel genes associated with or responding to iron deficiency. Sixty-nine genomic regions associated with IDC tolerance were identified across 19 chromosomes via the GWAS, including the major-effect quantitative trait locus (QTL) on chromosome Gm03. Cluster analysis of significant SNPs in this region deconstructed this historically prominent QTL into four distinct linkage blocks, enabling the identification of multiple candidate genes for iron chlorosis tolerance. The complementary GWES identified SNPs in this region interacting with nine other genomic regions, providing the first evidence of epistatic interactions impacting iron deficiency tolerance. Conclusions: This study demonstrates that integrating cutting edge genome wide association (GWA), genome wide epistasis (GWE), and gene expression studies is a powerful strategy to identify novel iron tolerance QTL and candidate loci from diverse germplasm. Crops, unlike model species, have undergone selection for thousands of years, constraining and/or enhancing stress responses. Leveraging genomics-enabled approaches to study these adaptations is essential for future crop improvement.

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“…To this effect, they undergo multiple UGGT-mediated reglucosylation cycles (2,(13)(14)(15). UGGT expression is increased upon ER stress and plays an important role in the unfolded protein response (16). The enzyme also surveys the assembly of key immunological molecules, the T-cell receptor (TCR) and the major histocompatibility complex (MHC).…”

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confidence: 99%

Interdomain conformational flexibility underpins the activity of UGGT, the eukaryotic glycoprotein secretion checkpoint

Roversi

Marti

Caputo

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Glycoproteins traversing the eukaryotic secretory pathway begin life in the endoplasmic reticulum (ER), where their folding is surveyed by the 170-kDa UDP-glucose:glycoprotein glucosyltransferase (UGGT). The enzyme acts as the single glycoprotein folding quality control checkpoint: it selectively reglucosylates misfolded glycoproteins, promotes their association with ER lectins and associated chaperones, and prevents premature secretion from the ER. UGGT has long resisted structural determination and sequence-based domain boundary prediction. Questions remain on how this single enzyme can flag misfolded glycoproteins of different sizes and shapes for ER retention and how it can span variable distances between the site of misfold and a glucose-accepting N-linked glycan on the same glycoprotein. Here, crystal structures of a full-length eukaryotic UGGT reveal four thioredoxin-like (TRXL) domains arranged in a long arc that terminates in two β-sandwiches tightly clasping the glucosyltransferase domain. The fold of the molecule is topologically complex, with the first β-sandwich and the fourth TRXL domain being encoded by nonconsecutive stretches of sequence. In addition to the crystal structures, a 15-Å cryo-EM reconstruction reveals interdomain flexibility of the TRXL domains. Double cysteine point mutants that engineer extra interdomain disulfide bridges rigidify the UGGT structure and exhibit impaired activity. The intrinsic flexibility of the TRXL domains of UGGT may therefore endow the enzyme with the promiscuity needed to recognize and reglucosylate its many different substrates and/or enable reglucosylation of N-linked glycans situated at variable distances from the site of misfold.

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The UDP-glucose: glycoprotein glucosyltransferase (UGGT), a key enzyme in ER quality control, plays a significant role in plant growth as well as biotic and abiotic stress in Arabidopsis thaliana

Cited by 64 publications

References 36 publications

Analysis of transcriptional response to heat stress in Rhazya stricta

Analysis of transcriptional response to heat stress in Rhazya stricta

Deconstructing the genetic architecture of iron deficiency chlorosis in soybean using genome-wide approaches

Interdomain conformational flexibility underpins the activity of UGGT, the eukaryotic glycoprotein secretion checkpoint

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