The U‐box E3 ubiquitin ligase PalPUB79 positively regulates ABA‐dependent drought tolerance via ubiquitination of PalWRKY77 in <i>Populus</i>

Tong, Shaofei; Chen, Ningning; Wang, Deyan; Ai, Fandi; Liu, Bao; Ren, Liwen; Yang, Chen; Zhang, Junlin; Lou, Shangling; Liu, Huanhuan; Liu, Jianquan; Ma, Tao; Jiang, Yuanzhong

doi:10.1111/pbi.13681

Cited by 28 publications

(24 citation statements)

References 61 publications

(90 reference statements)

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“…A total of 15 HB genes belonged to the HB-OTHERS subfamily (Table S3). In addition, the HB-PHD subfamily contained the fewest members (Table S3), which is similar to the results in rice (2), soybean (6) and Chinese cabbage (2) [10,37,38].…”

Section: Identification and Classification Of Hb Genes In P Trichocarpasupporting

confidence: 85%

“…To verify the expression patterns of PtrHB1 , PtrHB3 , PtrHB5 and PtrHB12 in poplars when dehydration occurs, mannitol treatment mimicking drought stress was performed [ 37 , 38 ]. The expression levels of these four genes were significantly up-regulated after treatment with 0.25 M mannitol ( Figure 8 A).…”

Section: Resultsmentioning

confidence: 99%

“…The poplar seeds ( P. alba ) were sterilized and directly germinated on MS medium for 7 days, and then we selected and transplanted the well-developed seedlings into nutrient soil. The seedlings were cultured in the greenhouse at 25 °C under light/darkness of 16/8 h with a light intensity of 4500 lux for the subsequent experiments, as previously reported [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

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Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in Populus trichocarpa

Hou

Sun

Wang

et al. 2021

Plants

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Homeobox (HB) genes play critical roles in the regulation of plant morphogenesis, growth and development. Here, we identified a total of 156 PtrHB genes from the Populus trichocarpa genome. According to the topologies and taxonomy of the phylogenetic tree constructed by Arabidopsis thaliana HB members, all PtrHB proteins were divided into six subgroups, namely HD-ZIP, ZF-HD, HB-PHD, TALE, WOX and HB-OTHERS. Multiple alignments of conserved homeodomains (HDs) revealed the conserved loci of each subgroup, while gene structure analysis showed similar exon–intron gene structures, and motif analysis indicated the similarity of motif number and pattern in the same subgroup. Promoter analysis indicated that the promoters of PtrHB genes contain a series of cis-acting regulatory elements involved in responding to various abiotic stresses, indicating that PtrHBs had potential functions in these processes. Collinearity analysis revealed that there are 96 pairs of 127 PtrHB genes mainly distributing on Chromosomes 1, 2, and 5. We analyzed the spatio-temporal expression patterns of PtrHB genes, and the virus-induced gene silencing (VIGS) of PtrHB3 gene resulted in the compromised tolerance of poplar seedlings to mannitol treatment. The bioinformatics on PtrHB family and preliminary exploration of drought-responsive genes can provide support for further study of the family in woody plants, especially in drought-related biological processes. It also provides a direction for developing new varieties of poplar with drought resistance. Overall, our results provided significant information for further functional analysis of PtrHB genes in poplar and demonstrated that PtrHB3 is a dominant gene regulating tolerance to water stress treatment in poplar seedlings.

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Section: Identification and Classification Of Hb Genes In P Trichocarpasupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in Populus trichocarpa

Hou

Sun

Wang

et al. 2021

Plants

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“…PalPUB79 enhanced drought tolerance and positively regulates hypersensitivity to ABA in transgenic poplars. PalPUB79 interacted with PalWRKY77 and mediated its ubiquitination for degradation, therefore counteracting its inhibitory effect on PalRD26 transcription [33]. These results show that the E3 ligase has biological functions diametrically opposed to those of its substrate, which is recognized explicitly and degraded by the 26S proteasome.…”

Section: Discussionmentioning

confidence: 85%

“…In addition, AtPUB11 was proposed as a negative regulator for drought tolerance by degrading the LRR1 and KIN7 RLKs [31]. AtPUB30 plays a role in decreasing salinity tolerance by regulating the degradation of BKI1 [32,33]. GmPUB6 and GmPUB8 negatively regulate the drought tolerance and play an essential role in osmotic stress and ABA signaling pathway in soybean [27,34].…”

Section: Introductionmentioning

confidence: 99%

The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean

Yang

Karthikeyan

Yin

et al. 2022

IJMS

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E3-ubiquitin ligases are known to confer abiotic stress responses in plants. In the present study, GmPUB21, a novel U-box E3-ubiquitin ligase-encoding gene, was isolated from soybean and functionally characterized. The expression of GmPUB21, which possesses E3-ubiquitin ligase activity, was found to be significantly up-regulated by drought, salinity, and ABA treatments. The fusion protein GmPUB21-GFP was localized in the cytoplasm, nucleus, and plasma membrane. Transgenic lines of the Nicotiana benthamiana over-expressing GmPUB21 showed more sensitive to osmotic, salinity stress and ABA in seed germination and inhibited mannitol/NaCl-mediated stomatal closure. Moreover, higher reactive oxygen species accumulation was observed in GmPUB21 overexpressing plants after drought and salinity treatment than in wild-type (WT) plants. Contrarily, silencing of GmPUB21 in soybean plants significantly enhanced the tolerance to drought and salinity stresses. Collectively, our results revealed that GmPUB21 negatively regulates the drought and salinity tolerance by increasing the stomatal density and aperture via the ABA signaling pathway. These findings improved our understanding of the role of GmPUB21 under drought and salinity stresses in soybean.

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RADseq‐based population genomic analysis and environmental adaptation of rare and endangered recretohalophyte Reaumuria trigyna

Dang

Liu

et al. 2023

The Plant Genome

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Genetic diversity reflects the survival potential, history, and population dynamics of an organism. It underlies the adaptive potential of populations and their response to environmental change. Reaumuria trigyna is an endemic species in the Eastern Alxa and West Ordos desert regions in China. The species has been considered a good candidate to explore the unique survival strategies of plants that inhabit this area. In this study, we performed population genomic analyses based on restriction‐site associated DNA sequencing to understand the genetic diversity, population genetic structure, and differentiation of the species. Analyses of 92,719 high‐quality single‐nucleotide polymorphisms (SNPs) indicated that overall genetic diversity of R. trigyna was low (HO = 0.249 and HE = 0.208). No significant genetic differentiation was observed among the investigated populations. However, a subtle population genetic structure was detected. We suggest that this might be explained by adaptive diversification reinforced by the geographical isolation of populations. Overall, 3513 outlier SNPs were located in 243 gene‐coding sequences in the R. trigyna transcriptome. Potential sites under diversifying selection occurred in genes (e.g., AP2/EREBP, E3 ubiquitin‐protein ligase, FLS, and 4CL) related to phytohormone regulation and synthesis of secondary metabolites which have roles in adaptation of species. Our genetic analyses provide scientific criteria for evaluating the evolutionary capacity of R. trigyna and the discovery of unique adaptions. Our findings extend knowledge of refugia, environmental adaption, and evolution of germplasm resources that survive in the Ordos area.

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The U‐box E3 ubiquitin ligase PalPUB79 positively regulates ABA‐dependent drought tolerance via ubiquitination of PalWRKY77 in Populus

Cited by 28 publications

References 61 publications

Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in Populus trichocarpa

Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in Populus trichocarpa

The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean

RADseq‐based population genomic analysis and environmental adaptation of rare and endangered recretohalophyte Reaumuria trigyna

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