The type and composition of alginate and hyaluronic-based hydrogels influence the viability of stem cells of the apical papilla

Lambricht, Laure; Berdt, Pauline De; Vanacker, Julie; Leprince, Julian; Diogenes, Aníbal; Goldansaz, Hadi; Bouzin, Caroline; Préat, Véronique; Dupont‐Gillain, Christine C.; Rieux, Anne des

doi:10.1016/j.dental.2014.08.369

Cited by 44 publications

(46 citation statements)

References 33 publications

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“…First of all, variations in structure, density and biodegradability between the two matrices may explain differences in vascular cell invasion (cell mobility). The 1% alginate SLM100 used in this study has a honeycomb structure with pores showing a 200 µm diameter and G' modulus of 4500 Pa [48], while the 30 mg/ml fibrinogen/30 IU/ml thrombin fibrin hydrogel forms a nanofibrous network with 1 µm pores and G' modulus of 900 Pa [49].…”

Section: Discussionmentioning

confidence: 99%

Transplantation of testicular tissue in alginate hydrogel loaded with VEGF nanoparticles improves spermatogonial recovery

Poels

Abou-Ghannam

Decamps

et al. 2016

Journal of Controlled Release

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Section: Discussionmentioning

confidence: 99%

Transplantation of testicular tissue in alginate hydrogel loaded with VEGF nanoparticles improves spermatogonial recovery

Poels

Abou-Ghannam

Decamps

et al. 2016

Journal of Controlled Release

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“…Therefore, there has been a surge of interest in the development of injectable hydrogels in recent years for tissue engineering [20], particularly dental pulp tissue engineering [8–12]. …”

Section: Discussionmentioning

confidence: 99%

“…While most studies [11, 12, 16] focus on modification of the scaffold to enhance odontogenic differentiation and biomineralization, the influence of matrix stiffness on the differentiation of DPSCs is still largely unclear. Separately, several studies have reported the influence of matrix stiffness on modulation of cell fate of a wide variety of cell types including stem cells [21–26].…”

Section: Discussionmentioning

confidence: 99%

“…In the context of dental pulp tissue engineering, the use of injectable hydrogels as scaffolds is particularly attractive as they are expected to conform to the variable shape of the pulp chamber and can be formulated with cells and/or growth factors by simple mixing [8–12]. We have previously developed an injectable semisynthetic hydrogel scaffold material comprised of a fibrinogen backbone covalently conjugated to polyethylene glycol (PEG) side chains and cross-linked by photopolymerization.…”

Section: Introductionmentioning

confidence: 99%

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Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System

Pandya

Rufaihah

et al. 2015

Stem Cells International

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Injectable hydrogels have the great potential for clinical translation of dental pulp regeneration. A recently developed PEG-fibrinogen (PF) hydrogel, which comprises a bioactive fibrinogen backbone conjugated to polyethylene glycol (PEG) side chains, can be cross-linked after injection by photopolymerization. The objective of this study was to investigate the use of this hydrogel, which allows tuning of its mechanical properties, as a scaffold for dental pulp tissue engineering. The cross-linking degree of PF hydrogels could be controlled by varying the amounts of PEG-diacrylate (PEG-DA) cross-linker. PF hydrogels are generally cytocompatible with the encapsulated dental pulp stem cells (DPSCs), yielding >85% cell viability in all hydrogels. It was found that the cell morphology of encapsulated DPSCs, odontogenic gene expression, and mineralization were strongly modulated by the hydrogel cross-linking degree and matrix stiffness. Notably, DPSCs cultured within the highest cross-linked hydrogel remained mostly rounded in aggregates and demonstrated the greatest enhancement in odontogenic gene expression. Consistently, the highest degree of mineralization was observed in the highest cross-linked hydrogel. Collectively, our results indicate that PF hydrogels can be used as a scaffold for DPSCs and offers the possibility of influencing DPSCs in ways that may be beneficial for applications in regenerative endodontics.

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“…Commercially available HA-Tyr (Corgel), was enzymatically crosslinked using a wellestablished method that results in the formation of a hydrogel with controlled properties. 19,23 The HA-Tyr polymer chains were covalently bound by using hydrogen peroxide (H 2 O 2 ) in the presence of horseradish peroxidase (HRP) enzyme. Figures 1g and h show the naked VACNT bundle sensor and the sensor with the HA-Tyr hydrogel cupula, respectively.…”

Section: Resultsmentioning

confidence: 99%

Biomimetic hydrogel-CNT network induced enhancement of fluid-structure interactions for ultrasensitive nanosensors

et al. 2017

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Flexible, self-powered, miniaturized, ultrasensitive flow sensors are in high demand for human motion detection, myoelectric prosthesis, biomedical robots, and health-monitoring devices. This paper reports a biomimetic nanoelectromechanical system (NEMS) flow sensor featuring a PVDF nanofiber sensing membrane with a hydrogel infused, vertically aligned carbon nanotube (VACNT) bundle that mechanically interacts with the flow. The hydrogel-VACNT structure mimics the cupula structure in biological flow sensors and gives the NEMS flow sensor ultrahigh sensitivity via a material-induced drag force enhancement mechanism. Through hydrodynamic experimental flow characterization, this work investigates the contributions of the mechanical and structural properties of the hydrogel in offering a sensing performance superior to that of conventional sensors. The ultrahigh sensitivity of the developed sensor enabled the detection of minute flows generated during human motion and micro-droplet propagation. The novel fabrication strategies and combination of materials used in the biomimetic NEMS sensor fabrication may guide the development of several wearable, flexible, and self-powered nanosensors in the future. NPG Asia Materials (2017) 9, e440; doi:10.1038/am.2017.183; published online 27 October 2017 INTRODUCTION Nature's designs have long inspired researchers aiming to find technical solutions and develop novel and advanced engineering systems. 1 Biological sensors are an example of a natural design that has intrigued scientists over the years. These sensors, for example, flow sensors, acoustic sensors, and chemical sensors, have developed high efficiencies, compactness, responsivity, and sensitivity through evolution over billions of years. 2 Most man-made artificial sensors scarcely match the performance of these natural sensors. Hence, for the development of novel sensors with high performance and sensitivity, it is desirable to apply designs from natural sensors to artificial engineering materials.Hair cell mechanoreceptors belong to a class of well-developed natural sensors ubiquitously found in many living organisms such as insects, amphibians, fishes, and mammals. 2 These natural transducers respond to a variety of environmental stimuli and are capable of sensing pressure, flow, acoustic waves, and chemicals. Some examples include air flow sensors in crickets, air flow and vibration sensors in spiders, water flow sensing whiskers in harbor seals, neuromast acceleration sensors in fishes, and cilia in the organs of mammals, including the taste buds on the tongue and cilia in the nose, lungs, and inner ear cochlea. [1][2][3] In aquatic environments, these hair cells have

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The type and composition of alginate and hyaluronic-based hydrogels influence the viability of stem cells of the apical papilla

Cited by 44 publications

References 33 publications

Transplantation of testicular tissue in alginate hydrogel loaded with VEGF nanoparticles improves spermatogonial recovery

Transplantation of testicular tissue in alginate hydrogel loaded with VEGF nanoparticles improves spermatogonial recovery

Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System

Biomimetic hydrogel-CNT network induced enhancement of fluid-structure interactions for ultrasensitive nanosensors

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