Transplantation of testicular tissue in alginate hydrogel loaded with VEGF nanoparticles improves spermatogonial recovery

Poels, Jonathan; Abou-Ghannam, Gaël; Decamps, Aline; Leyman, Mélanie; Rieux, Anne des; Wyns, Christine

doi:10.1016/j.jconrel.2016.05.037

Cited by 53 publications

(43 citation statements)

References 57 publications

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“…Our data show that VEGF had a profound effect on the vascular surface and vessel density of the transplants from both donor 1 and donor 2. In a previous study, mouse testis transplants supplemented with alginate-VEGF nanoparticles showed a significantly higher vascular surface at 5 and 21 days post-transplantation (Poels et al, 2016). Furthermore, the blockage of the VEGF/VEGFR2 pathway by daily exogenous administration of the SU5416 antagonist led to disruption of angiogenesis and a substantial reduction of the number of blood vessels in regenerated mouse testis tissue (Tian et al, 2016).…”

Section: Discussionmentioning

confidence: 84%

“…A tendency for better tubular integrity has been reported in mouse testis tissue transplants when tissue was embedded in hydrogel loaded with VEGF nanoparticles. The beneficial effect, however, was transient as it was present at 5 but not at 21 days posttransplantation (Poels et al, 2016).…”

Section: Discussionmentioning

confidence: 93%

“…Administration of VEGF, either by culture of the tissue before transplantation or by hydrogel delivery, promoted angiogenesis, follicular survival and enhanced the function of ovarian tissue xenotransplants, including human (Shikanov et al, 2011;Labied et al, 2013;Wang et al, 2013;Henry et al, 2015;Langbeen et al, 2016;Tavana et al, 2016). A previous study on autotransplantation of mouse ITT revealed a positive short-term effect of VEGF on transplant neovascularization 5 and 21 days post-transplantation (Poels et al, 2016), whereas the enhancement of germ cell survival has been demonstrated in several species, including mouse, pig and bovine (Schmidt et al, 2006;Caires et al, 2009Caires et al, , 2012Dores and Dobrinsky, 2014;Poels et al, 2016, Tian et al, 2016.…”

Section: Introductionmentioning

confidence: 92%

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Effect of recombinant human vascular endothelial growth factor on testis tissue xenotransplants from prepubertal boys: a three-case study

Ntemou

Kadam

Laere

et al. 2019

Reproductive BioMedicine Online

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 92%

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Effect of recombinant human vascular endothelial growth factor on testis tissue xenotransplants from prepubertal boys: a three-case study

Ntemou

Kadam

Laere

et al. 2019

Reproductive BioMedicine Online

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“…With regard to oncological patients, those with non-metastasising solid tumours could also come into consideration after tissue examination to exclude the presence of cancer cells in the tissue to be transplanted (providing that reliable techniques become available) and after fully informed patient-doctor decision and consent process. Currently, research is still ongoing to optimise tissue transplantation outcome as xenotransplantation experiments with human immature testicular tissue demonstrate a significant loss of spermatogonia, most likely due to hypoxia related to the absence of a vascular anastomosis between graft and host [18,32,33].…”

Section: Perspectives and Challenges Of Fertility Restoration Strategiesmentioning

confidence: 99%

Fertility sparing strategies for pre- and peripubertal male cancer patients

Stukenborg

Wyns

2020

ecancer

Self Cite

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Genetic parenthood following cancer therapy is considered to be a major factor of quality of life. Given the rising proportion of patients surviving cancer due to improved therapeutic protocols, it is an issue of growing importance. Hence, the efforts to preserve fertility have motivated researchers to develop options for the paediatric population facing fertility-threatening cancer therapies. In prepubertal boys who do not yet produce sperm, cryo-banking of testicular tissue containing spermatogonial stem cells (SSCs) is the only viable option for future fertility preservation. While proposed in a number of clinics worldwide, however, this strategy remains still experimental. Transplanting the SSCs, or testicular tissue containing SSCs, back to the cured patient appears the most promising strategy. However, experiments performed with human testicular tissue in mice models reveal spermatogonial loss after transplantation, indicating the need for further optimisation of the transplantation procedure. The approach further poses the risk of reintroducing tumour cells back to the patient. In cases of haematological and blood-metastasising malignancies, in vitro generation of sperm combined with assisted reproductive technologies (ART), is the only possibility, avoiding reintroducing cancer cells. Although xenotransplantation would allow to recover sperm cells for ART being thus on the safe side with regard to cancer cells, the risk of infections with xeno-microbiological agents makes this option incompatible with clinical application. So far, offspring from in vitro matured sperm has only been achieved in mice. While human haploid germ cells, showing specific morphological features, expression of post-meiotic markers, as well as DNA and chromosome content, as well as fertilisation and development capacity, have been obtained by culturing spermatogonia or immature testicular tissue, the functionality of these cells still needs to be demonstrated. Despite the promising results obtained in recent years, further research is urgently warranted to establish a clinical tool offering these boys a fertility restoration option in the future. This minireview will focus on current achievements and future challenges of fertility preservation in young boys and underscore the next steps required to translate experimental strategies into clinical practice.

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“…The combination of SBA with a natural polymer can result in an advanced biomaterial whose properties can be modulated into a specific microenvironment. Alginate has received a great deal of attention as a biomaterial, owing to its advantages, such as biocompatibility and biodegradability (Poels et al, 2016). This polysaccharide is obtained from alginic acid which possesses several possible arrangements of chains because it has two different kinds of monomeric unities, both containing a carboxylic group in each.…”

Section: Introductionmentioning

confidence: 99%

Bionanocomposites based on mesoporous silica and alginate for enhanced drug delivery

Lima

Kupfer

Moisés

et al. 2018

Carbohydrate Polymers

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This work reports the preparation, the characterization and the prednisolone release profile of biocompatible hydrogel nanocomposites containing mesoporous silica (SBA) and alginate as a biomaterial for enhanced drug delivery with reduced burst effect and improved mechanical properties. Such systems, which were prepared using specific SBA/alginate-crosslinking chemistry, exhibited interconnecting pore hybrid network owing to both mesoporous silica and hydrogel characteristics. Activated SBA was shown to be a determinant factor in inhibiting initial burst by nearly 90% and the drug was released with minimal burst kinetics. The nanoparticles reduced the movements of polymer chains, affecting macromolecular relaxation, and the distribution of mesoporous silica within the hydrogel made drug release into surrounding liquid less favorable. The proposed systems are biocompatible with human immortalized RWPE-1 prostatic epithelial cells. This report offers an approach of up-to-date interest for the development of advanced biomaterials for further physiological and pathological applications.

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Transplantation of testicular tissue in alginate hydrogel loaded with VEGF nanoparticles improves spermatogonial recovery

Cited by 53 publications

References 57 publications

Effect of recombinant human vascular endothelial growth factor on testis tissue xenotransplants from prepubertal boys: a three-case study

Effect of recombinant human vascular endothelial growth factor on testis tissue xenotransplants from prepubertal boys: a three-case study

Fertility sparing strategies for pre- and peripubertal male cancer patients

Bionanocomposites based on mesoporous silica and alginate for enhanced drug delivery

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