The trophic effect of ouabain on retinal ganglion cells is mediated by IL-1β and TNF-α

von-Held-Ventura, Juliana Salles; Mázala-de-Oliveira, Thalita; Oliveira, Amanda Cândida da Rocha; Granja, Marcelo Gomes; Gonçalves-de-Albuquerque, Cassiano Felippe; Castro‐Faria‐Neto, Hugo C.; Giestal-de-Araujo, Elizabeth

doi:10.1016/j.bbrc.2016.07.043

Cited by 13 publications

(12 citation statements)

References 27 publications

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“…Thus, we can hypothesize that, in neutrophils, ouabain 100 nM partially inhibits sodium pump and does not trigger p38 MAPK signaling directly. In agreement with other works that show ouabain biological effect in other models in low concentrations/doses (De Rezende Corrêa et al 2005 ; Salles von-Held-Ventura et al 2016 ). However, an experimental approach needs to be done to answer this hypothesis.…”

Section: Discussionsupporting

confidence: 93%

Ouabain inhibits p38 activation in mice neutrophils

et al. 2021

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Section: Discussionsupporting

confidence: 93%

Ouabain inhibits p38 activation in mice neutrophils

et al. 2021

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“…7 Thus, in our study the decrease of circulating CD4 + CD25 − CD39 + mTeff cells can be due to apoptosis or redistribution to peripheral tissues. Similarly to us, Minuzzi et al 33 showed lower frequencies of CD4 + CD27 − CD45ra − KLRG1 + (effector memory) and CD4 + KLRG1 + T cells 1 hour after exercise in masters athletes.…”

Section: Discussionsupporting

confidence: 65%

Physical fitness modulates the expression of CD39 and CD73 on CD4⁺ CD25⁻ and CD4⁺ CD25⁺ T cells following high intensity interval exercise

Dorneles¹,

Silva²,

Peres

et al. 2019

J of Cellular Biochemistry

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Aim: To investigate the impact of physical fitness on the mobilization of CD4 + CD25 − CD39 + and CD4 + CD25 + CD39 + T cells in response to acute exercise.Methods: Fifteen high physical fitness (25.3 ± 1.4 years) and 15 low physical fitness (26.1 ± 1.9 years) men performed a single bout of high-intensity interval exercise (HIIE, 10 bouts of 60 seconds at 85% HRmax intercepted by 75 seconds of recovery at 50% HRmax). Blood lymphocytes were isolated before, immediately after and 1 hour after exercise for assessment of cell surface expression of CD25, CD39, and CD73 on CD4 + T cells. Effector memory T cells (mTeff) were identified by CD4 + CD25 − CD39 + coexpression, and memory regulatory T cells (mTReg) were defined as CD4 + CD25 + CD39 + T cells.Results: Exercise increased CD4 + and CD4 + CD25 + T cell frequencies immediately after followed by a decrease bellow to baseline values at 1 hour after the bout in both low and high physical fitness groups. At baseline, the proportions of mTeff were higher, while mTreg were lower in low physical fitness individuals. The frequency of mTreg increased immediately after HIIE in both groups, and remained higher 1 hour after the bout. However, high physical fitness individuals presented higher mTreg frequency in all periods evaluated. A significantly mobilization of mTeff cells was identified in both groups immediately after HIIE. High physical fitness individuals displayed a decrease in mTeff cells bellow to baseline, while the frequency of mTeff remained higher in low physical fitness group 1 hour after the bout. The peripheral frequency of CD4 + CD25 + CD73 + T cells increased in a similar way immediately after the bout in both groups, returning to the baseline values 1 hour after exercise. No differences in CD4 + CD25 − CD73 + T cells were observed after HIIE in both groups. Conclusion:Our results highlight the impact of physical activity status in the redistribution of CD4 + T cells expressing ectonucleotidases in response to HIIE. K E Y W O R D Saerobic fitness, effector memory T cells, interval exercise, memory regulatory T cells

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“…The activation of NF-κB leads to secretion of the pro-inflammatory mediators TNF-α and IL-1β ( 36 ). IL-1β and TNF-α are usually considered pro-inflammatory mediators and have important roles in the immunologic system ( 37 ). In the current research, we found that knockdown of the MHC-I heavy chain increased the expression of the pro-inflammatory mediators TNF-α and IL-1β in LPS-stimulated bEECs.…”

Section: Discussionmentioning

confidence: 99%

IFN-τ Mediated Control of Bovine Major Histocompatibility Complex Class I Expression and Function via the Regulation of bta-miR-148b/152 in Bovine Endometrial Epithelial Cells

Jiang

Guo

et al. 2018

Front. Immunol.

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IFN-τ, a type I interferon produced by the trophoblasts of ruminants, has various important immune functions, including effects on the expression of major histocompatibility complex (MHC) class I (MHC-I). A previous study has reported that IFN-τ promotes the expression of MHC-I molecules on endometrial cells. However, the immunological mechanisms by which IFN-τ regulates MHC-I molecules remain unknown. Here, we investigated which microRNA (miRNAs) may be involved in the regulation of MHC-I molecule expression and function in bovine endometrial epithelial cells (bEECs). By using TargetScan 6.2 and , two miRNAs were suggested to target the 3′UTR of the bovine MHC-I heavy chain: bta-miR-148b and bta-miR-152. Dual luciferase reporter and miRNA mimic/inhibitor assays suggested that bta-miR-148b/152 were negatively correlated with bovine MHC-I heavy chain genes. The function of the MHC-I heavy chain was then investigated using qRT-PCR, ELISA, western blotting, immunofluorescence, and RNA interference assays in primary bEECs and an endometrial epithelial cell line (BEND). The results demonstrated that bta-miR-148b/152 could promote TLR4-triggered inflammatory responses by targeting the bovine MHC-I heavy chain, and the MHC-I molecule negatively regulated TLR4-induced inflammatory reactions may through the Fps-SHP-2 pathway. Our discovery offers novel insight into negative regulation of the TLR4 pathway and elucidates the mechanism by which bovine MHC-I molecules control congenital inflammatory reactions.

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The trophic effect of ouabain on retinal ganglion cells is mediated by IL-1β and TNF-α

Cited by 13 publications

References 27 publications

Ouabain inhibits p38 activation in mice neutrophils

Ouabain inhibits p38 activation in mice neutrophils

Physical fitness modulates the expression of CD39 and CD73 on CD4⁺ CD25⁻ and CD4⁺ CD25⁺ T cells following high intensity interval exercise

IFN-τ Mediated Control of Bovine Major Histocompatibility Complex Class I Expression and Function via the Regulation of bta-miR-148b/152 in Bovine Endometrial Epithelial Cells

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The trophic effect of ouabain on retinal ganglion cells is mediated by IL-1β and TNF-α

Cited by 13 publications

References 27 publications

Ouabain inhibits p38 activation in mice neutrophils

Ouabain inhibits p38 activation in mice neutrophils

Physical fitness modulates the expression of CD39 and CD73 on CD4+ CD25− and CD4+ CD25+ T cells following high intensity interval exercise

IFN-τ Mediated Control of Bovine Major Histocompatibility Complex Class I Expression and Function via the Regulation of bta-miR-148b/152 in Bovine Endometrial Epithelial Cells

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Physical fitness modulates the expression of CD39 and CD73 on CD4⁺ CD25⁻ and CD4⁺ CD25⁺ T cells following high intensity interval exercise