The Trichophytin Test

Trichophytin Extraction: Biological Comparison of Trichophytin Extracted from Trichophyton mentagrophytes Grown in a Complex Medium and a Defined Medium

“…Defined (i) 18.5/45 (11-25) (20-70) 4.2 (0-13) 2.0 (0 -11) Experienced-immune 15 Cruickshank 10.1/4.2 (7-15) (0-12) 6.0 (0-8) 10.0 (5-17) (DTH only) CM107 10.4/3.6 (8-11) (2-13) 6.4 (0-7) 10.2 (7)(8)(9)(10)(11)(12)(13)(14)(15) Defined (i) 10.8/5.2 (6-11) (2-13) 6.7 (0-7) 10.4 (5)(6)(7)(8)(9)(10)(11)(12)(13) a Mean diameter of the wheal/flare (mm). b Range of wheal reactions.…”

“…Human skin test data. A common problem encountered when using commercial trichophytin or crude extracts is the percentage of false positives due to primary irritants contained in such skin test materials (5,(9)(10)(11)(12). None of the noninfected guinea pigs or the six virginal subjects manifested an initial wheal or flare reaction greater than 10 mm.…”

“…Subsequently, trichophytins were prepared from both supernatant and mycelial mats of dermatophytes grown in various types of complex media by various crude extraction procedures (2)(3)(4)(5)(6)(7)(8). These procedures resulted in complex and nonstandardized trichophytins which elicited false negative and false positive skin tests (5,(8)(9)(10)(11)(12). Cruickshank et al (12)(13)(14)(15)) demonstrated that their glycopeptide antigen elicited allergic reactions in infected guinea pigs.…”