“…Subsequently, trichophytins were prepared from both supernatant and mycelial mats of dermatophytes grown in various types of complex media by various crude extraction procedures (2)(3)(4)(5)(6)(7)(8). These procedures resulted in complex and nonstandardized trichophytins which elicited false negative and false positive skin tests (5,(8)(9)(10)(11)(12). Cruickshank et al (12)(13)(14)(15)) demonstrated that their glycopeptide antigen elicited allergic reactions in infected guinea pigs.…”