The treatment with the probiotic Shewanella putrefaciens Pdp11 of specimens of Solea senegalensis exposed to high stocking densities to enhance their resistance to disease

Tapia‐Paniagua, Silvana; Vidal, Sara; Lobo, C.; Prieto‐Álamo, María‐José; Jurado, Juan; Cordero, Héctor; Cerezuela, Rebeca; Banda, Inés García de la; Esteban, M. Ángeles; Balebona, M.C.; Moriñigo, Miguel A.

doi:10.1016/j.fsi.2014.08.019

Cited by 74 publications

(50 citation statements)

References 79 publications

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“…Isolated and identified LAB from the intestine of farmed gilthead seabream fed with a commercial diet, whereas in our study the predominant bands related to LAB were not demonstrated in the DGGE patterns of fish fed the control diet. However [64,65], analysed the intestinal microbiota using a culture medium, such as Man Rogosa and Shape Agar, to select Lactobacillus species. In this way, although the level of LAB in the gut of fish could have been very low, the possibility to detect them increased very much.…”

Section: Discussionmentioning

confidence: 99%

Modulation of immunity and gut microbiota after dietary administration of alginate encapsulated Shewanella putrefaciens Pdp11 to gilthead seabream (Sparus aurata L.)

Cordero

Guardiola

Tapia‐Paniagua

et al. 2015

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 99%

Modulation of immunity and gut microbiota after dietary administration of alginate encapsulated Shewanella putrefaciens Pdp11 to gilthead seabream (Sparus aurata L.)

Cordero

Guardiola

Tapia‐Paniagua

et al. 2015

Fish & Shellfish Immunology

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“…It was reported that nccrp1 is up-regulated by overcrowding stress [8]. Besides, other study with probiotics has demonstrated up-regulation of…”

Section: Accepted M Manuscriptmentioning

confidence: 95%

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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Differential proteome profile of skin mucus of gilthead seabream (sparus aurata) after probiotic intake and/or overcrowding stress, Journal of Proteomics (2015Proteomics ( ), doi: 10.1016Proteomics ( /j.jprot.2015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E DM A N U S C R I P T ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 AbstractGilthead seabream (Sparus aurata L.) is the major cultured fish species in the Mediterranean area. High density stocking causes stress and increases the impact of diseases leading to economic losses. Probiotics could represent a solution to prevent diseases through several mechanisms such as improving the immune status and/or mucosal microbiota or competing with pathogens. The probiotic Shewanella putrefaciens, also known as Pdp11, was firstly isolated from the skin of healthy gilthead seabream. Our study focuses on the skin mucus proteome after dietary probiotic Pdp11 intake in fish maintained under normal or overcrowding conditions. 2-DE of skin mucus followed by LC-MS/MS analysis was done for each experimental group and differentially expressed proteins were identified. The results showed differentially expressed proteins especially involved in immune processes, such as lysozyme, complement C3, natural killer cell enhancing factor and nonspecific cytotoxic cell receptor protein 1, whose transcript profiles were studied by qPCR. A consistency between lysozyme protein levels in the mucus and lysozyme mRNA levels in skin were found. Further research is necessary to unravel the implications of skin mucosal immunity on fish welfare and disease. Biological significanceThe present work reveals the proteomic changes, which are taking place in the skin mucus of stressed and non-stressed gilthead seabream after Pdp11 probiotic intake. The study contributes to improve the knowledge on skin mucosal immunology of this relevant farmed fish species.Furthermore, the paper shows for the first time how a suitable proteomic methodology, in this case 2-DE followed by LC-MS/MS is useful to perform a comparative study with a noninvasive technique of skin mucus of gilthead seabream.

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“…For this reason, the development of tools that are focused on reducing the detectable adverse effects is now one of the priorities for the aquaculture M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 5 industry. One of the possible strategies to be applied in fish production could be the use of probiotics such as Shewanella putrefaciens Pdp11 (SpPdp11), which has a remarkable ability to modulate intestinal microbiota, thereby improving the liver condition in farmed Senegalese sole [29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Dietary administration of the probiotic SpPdp11: Effects on the intestinal microbiota and immune-related gene expression of farmed Solea senegalensis treated with oxytetracycline

Tapia‐Paniagua

Vidal

Lobo

et al. 2015

Fish & Shellfish Immunology

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The treatment with the probiotic Shewanella putrefaciens Pdp11 of specimens of Solea senegalensis exposed to high stocking densities to enhance their resistance to disease

Cited by 74 publications

References 79 publications

Modulation of immunity and gut microbiota after dietary administration of alginate encapsulated Shewanella putrefaciens Pdp11 to gilthead seabream (Sparus aurata L.)

Modulation of immunity and gut microbiota after dietary administration of alginate encapsulated Shewanella putrefaciens Pdp11 to gilthead seabream (Sparus aurata L.)

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Dietary administration of the probiotic SpPdp11: Effects on the intestinal microbiota and immune-related gene expression of farmed Solea senegalensis treated with oxytetracycline

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