The Transposon‐Like Structure of IS26‐Tetracycllne, and Kanamycin Resistance Determinant Derived from Transferable R Plasmid of Fish Pathogen, Pasteurella piscicida

Abstract

Tetracycline (pp-tet), and kanamycin (pp-kan) resistance genes were cloned from a transferable R plasmid of fish pathogen Pasteurella piscicida, and complete nucleotide sequences were determined. The pp-tet was a class D Tet determinant constructed with the tetA resistance gene of 1,182 by encoding a protein with a deduced molecular mass of 41 kDa and the tetR repressor gene of 654 by encoding a product of 24 kDa. The pp-tet was highly homologous to the tet(D) of plasmid RA1 isolated from Aeromonas hydrophila with two nucleotide differences in the tetR, and of plasmid pIP173 from Salmonella ordonez with two nucleotide differences in the tetA. The pp-kan contained 813 by encoding a 31 kDa protein of 271 amino acids, and was classified into type aph-Ic. It was identical to the aphA 7 in the IAB operon of pBWH77, in which was originally found an isolate of Klebsiella pneumoniae, in its nucleotide sequences and hybrid promoter construction. The genes were connected by an insertion sequence IS 26 of 820 bp, and were flanked by repeated copies in direct orientation at the 3' flanking region of the pp-tetA and in inverted orientation at the 3' flanking region of the pp-kan. The genetic elements are organized like a complex transposon by close linkage of the 1S26 and the pp-tet and -kan.

Emergence of Salmonella epidemics: The problems related to Salmonella enterica serotyp Enteritidis and multiple antibiotic resistance in other major serotypes

“…In addition, the class G tetracycline resistance gene associated with the floR gene in the S. Typhimurium DT104 antibiotic resistance gene cluster was first identified in Vibrio anguillarum, also a fish pathogen [157]. The tet(G) gene has also been detected on plasmids of Photobacterium damselae [81]. Based on these data, and on the fact that the floR and tet(G) genes in S. Typhimurium DT104 have a similar G+C content (58%), Angulo et al [3] suggested that the resistance determinants of multiple-antibiotic resistant S. Typhimurium DT104 strains have the same origin and may have emerged amongst bacteria in aquaculture and subsequently been horizontally transferred to S. Typhimurium DT104.…”

Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica Typhimurium DT104

“…In addition, the class G tetracycline resistance gene associated with the floR gene in the DT104 antibiotic resistance gene cluster was first identified in Vibrio anguillarum, also a fish pathogen [56]. The tet(G) gene has also been detected on plasmids of Photobacterium damselae [27]. The floR and tet(G) genes in S. enterica Typhimurium DT104 have a similar G + C content (58%) and could thus have the same origin.…”

Similarity in the Structure of <i>tetD</i>-Carrying Mobile Genetic Elements in Bacterial Strains of Different Genera Isolated from Cultured Yellowtail

“…PCR with a set of primers TCr3-F1 5 -AGCCTGACCAATCTGAC-3 and TCr3-R5 5 -GTGAAGAAGTGGCAGATC-3 described in Fig.2, and TA3 as a template DNA indicated the presence of adhC and ORF2, and IS26-Right in opposite orientation to the IS26-Left Fig.2A . The flanking region by IS26-Left and -Right was identical to the structure found in Photobacterium damselae Kim et al, 2008;Kim and Aoki, 1994 except for the ISRSB101. The inserted ISRSB101 was 100% identical to that of plasmid pFBOAT6 of Aeromonas punctata Rhodes et al, 2004 , and was bordered by direct repeats of 9 nucleotide 5 -TGTTGCAAA-3 originating from IS26-Inner.…”

“…TA3 pTCKM3 . Solid bars indicate the regions which exhibit homology to the reported sequences; pP99-018 from Photobacterium damselae Kim et al, 2008;Kim and Aoki, 1994 , Salmonella serovar Ordnez Allard et al, 1993 , and pFBAOT6 from Aeromonas punctata Rhodes et al, 2004 . B The structure found in Salmonella sp.…”

“…TC67 pCM67-5 . Solid bars indicate the regions which exhibit homology to the reported sequences; pP99-018 from Photobacterium damselae Kim et al, 2008;Kim and Aoki, 1994 , class I integron from Aeromonas hydrophila Poole et al, 2006 , TnSF1 form Shigella flexneri accession no. AF188331 , pPCVM196330_110 from Salmonella serovar Schwarzenground accession no.…”