The transport systems of mammalian lysosomes

Pisoni, Ronald L.; Thoene, Jess G.

doi:10.1016/0304-4157(91)90002-e

Cited by 108 publications

(81 citation statements)

References 130 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some processes in organisms have been identifi ed to require an acidic environment generated by V-ATPase (Forgac, 2007). The degradation of viral macromolecules might be recognized and sorted to lysosomes by antiviral immune responses of the resistant strain after BmNPV infection in the resistant silkworm strain, because acidifi cation of late endosomes by V-ATPase is important in intracellular traffi cking of newly synthesized lysosomal enzymes from the Golgi apparatus to lysosomes (Forgac, 2007), and the processing of lysosomal enzymes through hydrolyses also requires optimal acidic pH (Hasilik, 1992;Pisoni and Thoene, 1991). The transcription regulation of the V-ATPase c subunit gene should be dependent on the potential transcriptional binding sites in the promoter region and the loop-stem structure in 3'-UTR analyzed above.…”

Section: Discussionmentioning

confidence: 99%

Expression and Localization of Bombyx mori V-ATPase 16 kDa Subunit c

Chen

Yao

et al. 2010

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

V-ATPase plays a central role in lepidopteran midgut ion transport physiology, and lepidopteran midgut turned out to be a model tissue for the study of V-ATPase. In the present study, the 5'-RACE method is used to obtain the 5'-UTR of V-ATPase c subunit gene from Bombyx mori. Sequence analysis of the promoter region and 3'-UTR of V-ATPase c subunit gene revealed that the transcription of the V-ATPase c subunit gene may be regulated by multi-ways. RT-PCR analysis showed that B. mori V-ATPase c subunit mRNA expresses in the whole developmental stages of B. mori. We also constructed a transient vector to determine the subcellular localization of the B. mori V-ATPase c subunit, and the result demonstrated that it is located in the membrane and some specifi c regions of BmN cells. Real-time PCR analysis further indicated that the c subunit mRNA expression was upregulated signifi cantly at 24 and 72 h in the midguts of resistant B. mori larvae after being inoculated with B. mori nucleopolyhedrovirus, suggesting that it may be related to the immune response against virus infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression and Localization of Bombyx mori V-ATPase 16 kDa Subunit c

Chen

Yao

et al. 2010

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

show abstract

“…It is widely accepted that a major role of these organelles in a wide variety of cell types is the disassembly of macromolecules through the use of enzymatic digestion in an acidic microenvironment (Chou et al, 1992;Pisoni and Thoene, 1991). In some cells such as renal proximal tubular cells, filtered plasma proteins are reabsorbed by endocytosis and are transferred to lysosomes for degradation (Gekle, 1998).…”

Section: Discussionmentioning

confidence: 99%

Heavy metal detoxification in crustacean epithelial lysosomes: role of anions in the compartmentalization process

Sterling

Mandal

Roggenbeck

et al. 2007

Journal of Experimental Biology

View full text Add to dashboard Cite

-. As a group, these experiments suggest the presence of an anion exchange mechanism exchanging monovalent for polyvalent anions. Polyvalent inorganic anions (SO 4 2-and PO 4 3-) are known to associate with metals inside vesicles and a detoxification model is presented that suggests how these anions may contribute to concretion formation through precipitation with metals at appropriate vesicular pH.

show abstract

“…Further, the lysosomal interior is acidic, which not only facilitates iron release from proteins, but also enhances the oxidative damage caused by iron [28]. Lysosomes also maintain a high concentration of the amino acid cysteine [29], which may participate in the reduction of iron :…”

Section: Figure 5 Effects Of Desferrioxamine and 22h-dipyridyl On Lymentioning

confidence: 99%

Apoptosis induced by exposure to a low steady-state concentration of H2O2 is a consequence of lysosomal rupture

Antunes

Cadenas

Brunk

2001

Biochemical Journal

188

138

View full text Add to dashboard Cite

We have re-examined the lysosomal hypothesis of oxidative-stress-induced apoptosis using a new technique for exposing cells in culture to a low steady-state concentration of H2O2. This steady-state technique mimics the situation in vivo better than the bolus-administration method. A key aspect of H2O2-induced apoptosis is that the apoptosis is evident only after several hours, although cells may become committed within a few minutes of exposure to this particular reactive oxygen species. In the present work, we were able to show, for the first time, several correlative links between the triggering effect of H2O2 and the later onset of apoptosis: (i) a short (15min) exposure to H2O2 caused almost immediate, albeit limited, lysosomal rupture; (ii) early lysosomal damage, and later apoptosis, showed a similar dose-related response to H2O2; (iii) both events were inhibited by pre-treatment with iron chelators, including desferrioxamine. This compound is known to be taken up by endocytosis only and thus to become localized in the lysosomal compartment. After exposure to oxidative stress, when cells were again in standard culture conditions, a time-dependent continuous increase in lysosomal rupture was observed, resulting in a considerably lowered number of intact lysosomes in apoptotic cells, whereas non-apoptotic cells from the same batch of oxidative-stress-exposed cells showed mainly intact lysosomes. Taken together, our results reinforce earlier findings and strongly suggest that lysosomal rupture is an early upstream initiating event, and a consequence of intralysosomal iron-catalysed oxidative processes, when apoptosis is induced by oxidative stress.

show abstract

The transport systems of mammalian lysosomes

Cited by 108 publications

References 130 publications

Expression and Localization of Bombyx mori V-ATPase 16 kDa Subunit c

Expression and Localization of Bombyx mori V-ATPase 16 kDa Subunit c

Heavy metal detoxification in crustacean epithelial lysosomes: role of anions in the compartmentalization process

Apoptosis induced by exposure to a low steady-state concentration of H2O2 is a consequence of lysosomal rupture

Contact Info

Product

Resources

About