Expression and Localization of Bombyx mori V-ATPase 16 kDa Subunit c

Abstract

V-ATPase plays a central role in lepidopteran midgut ion transport physiology, and lepidopteran midgut turned out to be a model tissue for the study of V-ATPase. In the present study, the 5'-RACE method is used to obtain the 5'-UTR of V-ATPase c subunit gene from Bombyx mori. Sequence analysis of the promoter region and 3'-UTR of V-ATPase c subunit gene revealed that the transcription of the V-ATPase c subunit gene may be regulated by multi-ways. RT-PCR analysis showed that B. mori V-ATPase c subunit mRNA expresses in the whole developmental stages of B. mori. We also constructed a transient vector to determine the subcellular localization of the B. mori V-ATPase c subunit, and the result demonstrated that it is located in the membrane and some specifi c regions of BmN cells. Real-time PCR analysis further indicated that the c subunit mRNA expression was upregulated signifi cantly at 24 and 72 h in the midguts of resistant B. mori larvae after being inoculated with B. mori nucleopolyhedrovirus, suggesting that it may be related to the immune response against virus infection.

Vacuolar (H+)‐ATPase subunit c is essential for the survival and systemic RNA interference response in Locusta migratoria

“…S1). Our observations are consistent with the published literature where similar observations were made in BmN cells from B. mori 26 . Additionally, Nelson reported that V‐ATPase is principally located in vacuolar membranes, including endosomes, lysosomes, and clathrin‐coated vesicles 27 .…”

“…Our observations are consistent with the published literature where similar observations were made in BmN cells from B. mori. 26 Additionally, Nelson reported that V-ATPase is principally located in vacuolar membranes, including endosomes, lysosomes, and clathrin-coated vesicles. 27 The localization of LmV-ATPase-c in endosomes suggests that it likely participates in various basic cellular processes, but further study is clearly needed to verify its exact functions.…”