The transfer of triazine resistance from Brassica napus L. to B. oleracea L. II. Morphology, fertility and cytology of the F1 hybrid

Ayotte, R.; Harney, Patricia M.; Machado, V. Souza

doi:10.1007/bf00036857

Cited by 21 publications

(9 citation statements)

References 12 publications

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“…Several investigations have focused on improving the interspeciWc hybridization eYciency within the family Brassicaceae (Ayotte et al 1987;Bajaj et al 1986;Inomata 1993;Rahman 2004;Takeshita et al 1980;Zhang et al 2004). These studies primarily focused on the method of embryo rescue, time of harvest of hybrid embryos (DAP), and type of culture media.…”

Section: Embryo Rescuementioning

confidence: 99%

Interspecific cross of Brassica oleracea var. alboglabra and B. napus: effects of growth condition and silique age on the efficiency of hybrid production, and inheritance of erucic acid in the self-pollinated backcross generation

et al. 2008

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InterspeciWc hybrids were produced from reciprocal crosses between Brassica napus (2n = 38, AACC) and B. oleracea var. alboglabra (2n = 18, CC) to introgress the zero-erucic acid alleles from B. napus into B. oleracea. The ovule culture embryo rescue technique was applied for production of F 1 plants. The eVects of silique age, as measured by days after pollination (DAP), and growth condition (temperature) on the eYciency of this technique was investigated. The greatest numbers of hybrids per pollination were produced under 20°/15°C (day/night) at 16 DAP for B. oleracea ($) £ B. napus crosses, while under 15°/10°C at 14 DAP for B. napus ($) £ B. oleracea crosses. Application of the ovule culture technique also increased the eYciency of BC 1 (F 1 £ B. oleracea) hybrid production by 10-fold over in vivo seed set. The segregation of erucic acid alleles in the selfpollinated backcross generation, i.e. in BC 1 S 1 seeds, revealed that the gametes of the F 1 and BC 1 plants carrying a greater number of A-genome chromosomes were more viable. This resulted in a signiWcantly greater number of intermediate and a smaller number of high-erucic acid BC 1 S 1 seeds.

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Section: Embryo Rescuementioning

confidence: 99%

Interspecific cross of Brassica oleracea var. alboglabra and B. napus: effects of growth condition and silique age on the efficiency of hybrid production, and inheritance of erucic acid in the self-pollinated backcross generation

et al. 2008

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“…Based on these results, we deduced that these plants were generated by unreduced male gametes from parental B. oleracea. Although the occurrence of unreduced male or female gametes is extremely low, this phenomenon has been reported in the family Brassicaceae (Ayotte et al 1988;Fominaya et al 2005). An alternative possibility is that the chromosome set was partially doubled by colchicine treatment (namely only C chromosome set was doubled).…”

Section: Discussionmentioning

confidence: 97%

Analysis of intergeneric sexual hybridization between transgenic Brassica oleracea and Sinapis alba

Zhang

Guan

et al. 2017

Euphytica

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Sinapis alba possesses a number of desirable traits that can be employed to broaden genetic variability in Brassica oleracea or B. napus. Using transgenic B. oleracea (CC, 2n = 18) as paternal plants and non-transgenic S. alba (SS, 2n = 24) as maternal plants, 11 hybrid plants were successfully obtained by using a combination of ovary culture and embryo rescue, while 2 seedlings of the reciprocal cross were generated but lost during the propagation. The hybridity of these plants was confirmed by flower color and other agronomic characteristics, chromosome counting, and pollen viability. Most of the intergeneric F 1 plants showed only one set each of S and C chromosome (SC, 2n = 21) and were male sterile, while a few plants were semi-fertile and had one set of S chromosomes but two sets of C chromosomes (CCS, 2n = 30). PCR, genomic Southern blotting and qRT-PCR for bar gene revealed that among the F 1 hybrid plants only 9.1% was bar positive and could be forwarded to F 2 and F 3 generation. The majority of F 3 plants obtained sufficient resistance to Alternaria brassicae. Thus, the generation of disease (A. brassicae) and herbicide (Bastar) resistant intergeneric hybrids was of importance for breeding program. The existence of bar gene in these intermediate materials will facilitate the identification of hybridity and transfer of S.alba traits into target genetic background.

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“…The latter may have originated from the fertilization of an unreduced male gamete of B. nigra with the reduced macrospore of B. rapa. This phenomenon was known to occur in the Brassiceae with a relatively high frequency (Heyn 1977;Ayotte et al 1988;Lefol et al 1996). Simultaneous occurrence of both male and female 2n gametes in one plant facilitated sexual polyploidization, which could improve hybrid fertility (Inomata 2002).…”

Section: Discussionmentioning

confidence: 97%

A semi-fertile interspecific hybrid of Brassica rapa and B. nigra and the cytogenetic analysis of its progeny

Sheng

Wen

Guo

et al. 2011

Genet Resour Crop Evol

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Black rot is a bacterial disease of Brassica rapa caused by Xanthomonas campestris pv. campestris (Xcc.). Sources of resistance to this disease within B. rapa are insufficient and control measures are limited, making the development of resistant breeding lines extremely important. Certain lines of B. nigra exhibit very high resistance to Xcc. For this study, an interspecific cross between Brassica rapa and B. nigra was performed, and a total of 6 F 1 hybrids were obtained through ovary culture. Five plants (H 1-5 ) were relatively slow-growing, entirely sterile, and had 18 chromosomes in the majority of pollen mother cells (PMCs). GISH analysis showed that most of the PMCs had 8 B. nigra chromosomes, which indicated the expected AB genomic constitution. The last plant (H 6 ) was partially fertile and the majority of PMCs contained 10 chromosomes of B. rapa and 16 chromosomes of B. nigra, indicating an ABB genomic constitution. Ovary culture techniques were not necessary for the development of the firstor second-backcross generation. Sequence-related amplified polymorphism analysis of F 1 , BC 1 , and BC 2 plants indicated that some fragments from B. nigra were lost, particularly in the genome of BC 1 / BC 2 in successive generation(s). The BC 2 plants expressing good resistance to Xcc. were observed.

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The transfer of triazine resistance from Brassica napus L. to B. oleracea L. II. Morphology, fertility and cytology of the F1 hybrid

Cited by 21 publications

References 12 publications

Interspecific cross of Brassica oleracea var. alboglabra and B. napus: effects of growth condition and silique age on the efficiency of hybrid production, and inheritance of erucic acid in the self-pollinated backcross generation

Interspecific cross of Brassica oleracea var. alboglabra and B. napus: effects of growth condition and silique age on the efficiency of hybrid production, and inheritance of erucic acid in the self-pollinated backcross generation

Analysis of intergeneric sexual hybridization between transgenic Brassica oleracea and Sinapis alba

A semi-fertile interspecific hybrid of Brassica rapa and B. nigra and the cytogenetic analysis of its progeny

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