The Transfer of Bovine J Blood‐Group Activity to Erythrocytes: Kinetic Studies

Wagner, Rolf; Oulevey, J.; Thiele, O. W.

doi:10.1111/j.1439-0442.1984.tb01319.x

Zentralblatt für Veterinärmedizin Reihe A

1984

DOI: 10.1111/j.1439-0442.1984.tb01319.x

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The Transfer of Bovine J Blood‐Group Activity to Erythrocytes: Kinetic Studies

Rolf Wagner

J. Oulevey

O. W. Thiele

Abstract: UAg-Details of the serological procedure of the quantitative and semiquantitative assay have been described recently (10). Protein AssayProtein was assayed by the method of LOWRY et al. (8) as described previously (6) or by measuring the optical density at 280nm using an absorption coefficient of 6.60 for a 1 YO protein

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1988

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Biochemistry of the J Blood Group Substance of Cattle

Thiele

1988

Journal of Veterinary Medicine Series A

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The bovine J blood‐group substance is dissolved in the serum. The total lipids extracted from serum containing the J antigen (termed J+ serum) show haptenic activity in the homologous bovine J system. The total lipids from J−serum, however, show no haptenic activity. The J+total lipids are, therefore, thought to contain the determinant group of the J antigen. In fractionation experiments the lipids to be tested were mixed with crude glycerophospholipids extracted from J− serum, since it has been known that auxiliary lipids are required for maximum haptenic effects. The haptenic activity is completely destroyed by periodate oxidation, it does not decrease, however, by deacylation of ester lipids. Various fractionation procedures in addition to deacylation led to a purified glycosphingolipid that proved to be highly active as a J hapten. The low‐density lipoproteins prepared from J+ serum as well as the total lipids extracted from these lipoproteins inhibit the hemolysis of J+ erythrocytes in the J system. These results suggest that the J blood‐group substance dissolved in bovine J+ serum is a lipoprotein and that its determinant group is a glycosphingolipid.

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Biochemistry of the J Blood Group Substance of Cattle

Thiele

1988

Journal of Veterinary Medicine Series A

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The Transfer of Bovine J Blood‐Group Activity to Erythrocytes: Kinetic Studies

Cited by 1 publication

References 17 publications

Biochemistry of the J Blood Group Substance of Cattle

Biochemistry of the J Blood Group Substance of Cattle

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