The transcriptome analysis on urea response mechanism in the process of ergosterol synthesis by Cordyceps cicadae

Su, Qihui; Zhang, Zhicai; Liu, Xiaocui; Wang, Feng

doi:10.1038/s41598-021-90377-2

Cited by 6 publications

(2 citation statements)

References 39 publications

(40 reference statements)

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“…Nitrogen sources were essential for the growth and nucleoside synthesis regulation of Cordyceps specie ( Shih et al, 2007 ; Su et al, 2021 ). In our study, C. cicadae mycelium had a various preference for nitrogen sources during growth and HEA synthesis.…”

Section: Discussionmentioning

confidence: 99%

Exploiting the roles of nitrogen sources for HEA increment in Cordyceps cicadae

Zhu,

Ruan,

et al. 2024

Front. Microbiol.

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Cordyceps cicadae, as a new food ingredient, is a valuable edible and medicinal fungi. However, its resources are severely depleted due to environmental limitations and excessive harvesting practices. N6-(2-hydroxyethyl) adenosine (HEA), as an important product of Cordyceps cicadae, has the potential to be used in medical industry due to its diverse disease curing potential. However, the disclosure of HEA synthesis still severely limited its application until now. In this study, the kinetic curves for adenosine and HEA under shaker fermentation were explored. The kinetics of HEA and adenosine production exhibited a competitive pattern, implicating a possibility of sharing a same step during their synthesis. Due to HEA as a derivative of nitrogen metabolism, the effect of different nitrogen sources (peptone, yeast extract, ammonium sulfate, diammonium oxalate monohydrate, ammonium citrate dibasic, and ammonium citrate tribasic) on HEA production in Cordyceps cicadae strain AH 10-4 had been explored under different incubation conditions (shaker fermentation, stationary fermentation, and submerged fermentation). Our results indicated that the complex organic nitrogen sources were found to improve the accumulation of HEA content under shaker fermentation. In contrast, the optimal nitrogen source for the accumulation of HEA under stationary fermentation and submerged fermentation was ammonium citrate tribasic. But submerged fermentation obviously shortened the incubation time and had a comparable capacity of HEA accumulation by 2.578 mg/g compared with stationary fermentation of 2.535 mg/g, implicating a possibility of scaled-up production of HEA in industry by submerged fermentation. Based on the dramatic HEA production by ammonium sulfate as nitrogen resources between stationary and shaker fermentations, alanine, aspartate and glutamate as well as arginine metabolic pathway were related to the production of HEA by comparative transcriptome. Further investigation indicated that glutamic acid, which is an analog of Asp, showed an optimum production of HEA in comparison with other amino acids.

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Section: Discussionmentioning

confidence: 99%

Exploiting the roles of nitrogen sources for HEA increment in Cordyceps cicadae

Zhu,

Ruan,

et al. 2024

Front. Microbiol.

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“…More than 260 healthcare products had been developed with S. hepiali as a raw material, with Jinshuibao capsules having the highest output value [2]. Cordycipitaceae species have produced a variety of metabolites, such as bassianolide [4], fumosorinone [5], ergosterol [6], 8-O-methylindigotide B [7], cordycerebroside B [8], cepharosporolides C [9], beauvericin [10], beauveriolide [11], oosporein [12], bassiatin [13], ophiocordin [14], cordycepin [15], oosporein [16] and cordycepic acid [17]. It can be seen that there is still a lot of room for exploration of the potential of Cordycipitaceae species to produce compounds.…”

Section: Introductionmentioning

confidence: 99%

Genomic Comparison of Two Species of Samsoniella with Other Genera in the Family Cordycipitaceae

Lu,

Wang,

Wang

et al. 2023

JoF

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Whole genomes of Samsoniella hepiali ICMM 82-2 and S. yunnanensis YFCC 1527 were sequenced and annotated, as well as compared with whole genome sequences of other species in the family Cordycipitaceae. S. hepiali ICMM 82-2, S. hepiali FENG and S. yunnanensis YFCC 1527 had 54, 57 and 58 putative secondary metabolite biosynthetic gene clusters, respectively. S. hepiali had one unique domain and S. yunnanensis YFCC 1527 six. Both S. hepiali and S. yunnanensis YFCC 1527 had curvupallide-B, fumosorinone and fujikurin putative biosynthetic gene clusters. C. javanica had biosynthetic gene clusters for fumonisin. The 14 genomes had common domains, namely A-P-C-P-C and KS-AT-DH-ER-KR-ACP. The A-P-C-P-C domain may be involved in the biosynthesis of dimethylcoprogen. The maximum likelihood and the Bayesian inference trees of KS-AT-DH-ER-KR-ACP were highly consistent with the multigene phylogenetic tree for the 13 species of Cordycipitaceae. This study facilitates the discovery of novel biologically active SMs from Cordycipitaceae using heterologous expression and gene knockdown methods.

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