The toxicity and histopathology of Bacillus thuringiensis Cry1Ba toxin to Spodoptera frugiperda (Lepidoptera, Noctuidae)

Pinto, Laura Massochin Nunes; Dörr, Natália C; Fiúza, Lidia Mariana

doi:10.1142/9789814322119_0031

Cited by 4 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Infection of the CPB larvae with Bt per os led to cell swelling, the formation of autophagosomes, and partial destruction of the intestinal microvilli. Our effect is consistent with the previously described action of Bt Cry toxins on the intestinal epithelial layer of Spodoptera frugiperda (Lepidoptera: Noctuidae) 90 . Interestingly, reintroduction of C. freundii into the gut of CPB larvae by the end of the first day of the experiment led to pronounced hypersecretion of the endoplasmic reticulum with the formation of apocrine vesicles and cell swelling.…”

Section: Discussionsupporting

confidence: 92%

“…Our effect is consistent with the previously described action of Bt Cry toxins on the intestinal epithelial layer of Spodoptera frugiperda (Lepidoptera: Noctuidae). 90 Interestingly, reintroduction of C. freundii into the gut of CPB larvae by the end of the first day of the experiment led to pronounced hypersecretion of the endoplasmic reticulum with the formation of apocrine vesicles and cell swelling. However, this did not lead to mass death of insects, and histological sections showed that after 24 h intestinal cell functions were partially restored.…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Citrobacter freundii, a natural associate of the Colorado potato beetle, increases larval susceptibility to Bacillus thuringiensis

Polenogova

Noskov

Artemchenko

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND We assume that certain representatives of gut microflora mediate immune changes during dysbiosis, accelerating septicemia caused by Bacillus thuringiensis. RESULTS Co‐introduction of Citrobacter freundii with Bacillus thuringiensis var. tenebrionis (morrisoni) (Bt) led to an increase in Colorado potato beetle (CPB) larval mortality to 69.0% (1.3–5×) and a synergistic effect was observed from day 1 to day 6. Ultrathin sections of the CPB midgut showed autophagosome formation and partial destruction of gut microvilli under the influence of Bt, which was accompanied by pronounced hypersecretion of the endoplasmic reticulum with apocrine vesicle formation and oncotic changes in cells under the action of C. freundii. The destruction of gut tissues was accompanied by suppression of detoxification processes under the action of the bacteria and a decrease (2.8–3.5×) in the concentration of lipid oxidation products during Bt infection. In the first hours post combined treatment, we registered a slight increase in the total hemocyte count (THC) especially a predomination (1.4×) of immune‐competent plasmatocytes. Oral administration of symbiotic and entomopathogenic bacteria to the CPB larvae significantly decreased the THC (1.4×) after 24 h and increased (1.1–1.5×) the detoxifying enzymes level in the lymph. These changes are likely to be associated with the destruction of hemocytes and the need to remove the toxic products of reactive oxygen species. CONCLUSION The obtained results indicate that feeding of C. freundii and B. thuringiensis to the CPB larvae is accompanied by tissue changes that significantly affect the cellular and humoral immunity of the insect, increasing its susceptibility to Bt. © 2022 Society of Chemical Industry.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 96%

Citrobacter freundii, a natural associate of the Colorado potato beetle, increases larval susceptibility to Bacillus thuringiensis

Polenogova

Noskov

Artemchenko

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…The present results were similar to those observed by toxin, late third instar larvae of the tolerant population of S. littoralis exhibited vacuolization of the epithelium and disintegration of both the peritrophic membrane and the striated border in comparison to the susceptible strain. The results are also in congruence with Pinto et al (2011), who investigated the insecticidal activity and the histopathological effects of bacterial suspension and purified Cry1Ba protein from B. thuringiensis strain 4412 in the midgut epithelial cells of S. frugiperda larvae. The result revealed that the tested strain of B. thuringiensis thuringiensis4412 was highly toxic with cent per cent mortality.…”

Section: Resultssupporting

confidence: 86%

Insecticidal activity and changes in midgut histology of the generalist herbivore, Spodoptera litura F. (Lepidoptera: Noctuidae) in response to seed extract of Annona squamosa Linn.

Muthu

Vishnupriya

et al. 2023

JANS

View full text Add to dashboard Cite

The tobacco caterpillar, Spodoptera litura F., is one of the most devastating, cosmopolitan polyphagous pests affecting major crops that significantly impact agricultural productivity. The present study aimed to evaluate the toxicological effect of seed extract of the medicinal plant, Annona squamosa L. and the histological effect on the midgut of Spodoptera litura F., under laboratory conditions. The crude methanolic extract of A. squamosa seed was tested against the third instar larvae of S. litura at five different concentrations viz., 0.5%, 1.0%, 1.5%, 2.0% and 2.5 % by leaf dip bioassay method. The seed extract exhibited larval mortality of 96.67%, 83.37 %,70%, 53.33% and 40 % at the concentrations 2.5 %, 2.0%, 1.5%, 1.0% and 0.5%, respectively.The results indicated that the response of larval mortality to the seed extract was dose-dependent. The dose of 0.5% showed the lowest mortality (40.00%), while the dose of 2.5 % showed maximum larval mortality (96.67%). Hence they were subjected to histological analysis. The anatomical sectioning of S. litura larval midgut treated with 0.5% concentration showed disruption in the peritrophic membrane and striated border of epithelial cells. The midgut of larvae treated with 2.5 % concentration showed an irregular epithelium and high vacuolization in the cytoplasmic cells. The results indicated that methanol extract of A. squamosa seed extract has the ability to cause changes in the midgut region, thereby affecting the digestion and nutrient absorption of S. litura larvae, which will lead to a debilitating effect on the development of larvae. Based on the present study A. squamosa seed extract will be a potential biopesticide for managing S. litura.

show abstract

Indica Rice Cultivar IRGA 424, Transformed With cry Genes of B. thuringiensis, Provided High Resistance Against Spodoptera frugiperda (Lepidoptera: Noctuidae)

et al. 2013

View full text Add to dashboard Cite

Plant expression of the entomopathogenic bacteria Bacillus thuringiensis cry gene has reduced the damage created by insect pests in several economically important cultures. For this study, we have conducted genetic transformation of the indica rice "IRGA 424", via Agrobacterium tumefaciens, using the B. thuringiensis cry1Aa and cry1B genes, with the objective of obtaining rice plants resistant to the insect pests from this culture. The gene constructions harbor the promoters maize proteinase inhibitor and ubiquitin. The results showed that high concentration of the hormone 2,4-dichlorophenoxyacetic acid and agarose as the gelling agent helped the production of embryogenic calli for the analyzed cultivar. More than 80% of the obtained transformed plants revealed the integration, using polymerase chain reaction, of the cry1Aa and cry1B genes. Analysis of the expression of the heterologous protein by Western blotting revealed the expression of the Cry1B delta-endotoxin in IRGA 424 plants transformed with the ubiquitin promoter. Data showed the production and dissemination of a high number of embryogenic calli in addition to obtaining plants transformed with the cry1Aa and cry1B genes until the reproductive phase. The feed bioassays with the transformed plants and Spodoptera frugiperda (JE Smith) larvae indicated high rates of mortality to the insect target. The highest corrected mortality rate achieved under laboratory conditions with Bt-rice plants transformed with the cry1B and cry1Aa genes was 94 and 84%, respectively. Thus, our results demonstrated the great potential of transformed Bt-rice plants in controlling the damage caused by these insect pests in rice paddy fields.

show abstract

The toxicity and histopathology of Bacillus thuringiensis Cry1Ba toxin to Spodoptera frugiperda (Lepidoptera, Noctuidae)

Cited by 4 publications

References 12 publications

Citrobacter freundii, a natural associate of the Colorado potato beetle, increases larval susceptibility to Bacillus thuringiensis

Citrobacter freundii, a natural associate of the Colorado potato beetle, increases larval susceptibility to Bacillus thuringiensis

Insecticidal activity and changes in midgut histology of the generalist herbivore, Spodoptera litura F. (Lepidoptera: Noctuidae) in response to seed extract of Annona squamosa Linn.

Indica Rice Cultivar IRGA 424, Transformed With <I>cry</I> Genes of <I>B. thuringiensis</I>, Provided High Resistance Against <I>Spodoptera frugiperda</I> (Lepidoptera: Noctuidae)

Contact Info

Product

Resources

About