The tooth on-a-chip: a microphysiologic model system mimicking the biologic interface of the tooth with biomaterials

França, Cristiane Miranda; Tahayeri, Anthony; Rodrigues, Nara Sousa; Ferdosian, Shirin; Puppin-Rontani, Regina Maria; Sereda, Grigoriy; Ferracane, Jack L.; Bertassoni, Luiz E.

doi:10.1039/c9lc00915a

Cited by 65 publications

(58 citation statements)

References 39 publications

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“…(D ) Conventional staining methods on fixed samples are also possible after several days and illustrate the remarkable effect of dental materials on pulp cells even after 7 days from their application. Reproduced from 58 . (E ) Endothelial cells cultured on the tooth-on-a-chip microdevice for 24 hours, either with (bottom right ) or without (bottom left ) perfusion of EDTA through the dentin tubules for 1 minute, showing a clear vasculogenic effect due to the presence of dentin matrix molecules and flow.…”

Section: Discussionmentioning

confidence: 99%

“…Understanding the participation of dentin on the formation of the dental pulp vasculature is an important step to enable the controllable regeneration of a functional tissue. One of the recent developments that seeks to address this goal, among other objectives, is the fabrication of a microfluidic device that our team has recently referred to as the tooth-ona-chip 58 . The tooth-on-a chip is a controllable model system that enables investigation of pulp-related mechanisms in vitro.…”

Section: The Tooth-on-a-chip and Microdevices To Study The Dental Pulmentioning

confidence: 99%

“…Our group has recently described the development and characterization of such a "tooth-on-a-chip" model in a recent article 58 , and a few characteristics are noteworthy. For instance, the model can be engineered to enable the formation of a controllable interface between the tooth and exogenous oral components (ie, bacteria, dental materials, saliva, oral care products) just like in a real cavity, where the dental pulp forms a direct interface with the dentin and indirect contact with the oral cavity via the dentin tubules.…”

Section: The Tooth-on-a-chip and Microdevices To Study The Dental Pulmentioning

confidence: 99%

“…response of odontoblasts in the pulp to acid etching, different monomer molecules, and the whole process of a 2-step adhesive system 58 , some of which are illustrated in Figure 7. These results offer a unique window of the dental pulp response to injury and show a diverse set of morphologic changes as the materials are interfaced with the tooth, which were previously unknown.…”

Section: The Tooth-on-a-chip and Microdevices To Study The Dental Pulmentioning

confidence: 99%

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Progress and Challenges in Microengineering the Dental Pulp Vascular Microenvironment

Bertassoni

2020

Journal of Endodontics

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Section: Discussionmentioning

confidence: 99%

Section: The Tooth-on-a-chip and Microdevices To Study The Dental Pulmentioning

confidence: 99%

Section: The Tooth-on-a-chip and Microdevices To Study The Dental Pulmentioning

confidence: 99%

Section: The Tooth-on-a-chip and Microdevices To Study The Dental Pulmentioning

confidence: 99%

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Progress and Challenges in Microengineering the Dental Pulp Vascular Microenvironment

Bertassoni

2020

Journal of Endodontics

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“…[ 32–34 ] Altogether, this technology represents a platform for building human physiological models and investigating drug discovery and toxicology research. [ 35–38 ] During the last decade, organs‐on‐a‐chip platforms have been widely used to mimic the physiological environment of different organs such as lung, [ 39,40 ] kidney, [ 41,42 ] heart, [ 43,44 ] intestine, [ 45,46 ] teeth, [ 47 ] and bone. There have been many review papers concerning the development of the general topic of organ‐on‐a‐chip, as well as specific platforms devoted to various organ systems in the literature.…”

Section: Introductionmentioning

confidence: 99%

Bone‐on‐a‐Chip: Microfluidic Technologies and Microphysiologic Models of Bone Tissue

Mansoorifar

Gordon

Bergan

et al. 2020

Adv Funct Materials

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Bone is an active organ that continuously undergoes an orchestrated process of remodeling throughout life. Bone tissue is uniquely capable of adapting to loading, hormonal, and other changes happening in the body, as well as repairing bone that becomes damaged to maintain tissue integrity. On the other hand, diseases such as osteoporosis and metastatic cancers disrupt normal bone homeostasis leading to compromised function. Historically, the ability to investigate processes related to either physiologic or diseased bone tissue is limited by traditional models that fail to emulate the complexity of native bone. Organ‐on‐a‐chip models are based on technological advances in tissue engineering and microfluidics, enabling the reproduction of key features specific to tissue microenvironments within a microfabricated device. Compared to conventional in vitro and in vivo bone models, microfluidic models, and especially organ‐on‐a‐chip platforms, provide more biomimetic tissue culture conditions, with increased predictive power for clinical assays. In this review, microfluidic and organ‐on‐a‐chip technologies designed for understanding the biology of bone as well as bone‐related diseases and treatments are reported. Finally, the authors discuss the limitations of the current models and point toward future directions for microfluidics and organ‐on‐a‐chip technologies in bone research.

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Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research

Guttenplan

Birgani

Giselbrecht

et al. 2021

Adv Healthcare Materials

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In recent years, the use of microfabrication techniques has allowed biomaterials studies which were originally carried out at larger length scales to be miniaturized as so-called "on-chip" experiments. These miniaturized experiments have a range of advantages which have led to an increase in their popularity. A range of biomaterial shapes and compositions are synthesized or manufactured on chip. Moreover, chips are developed to investigate specific aspects of interactions between biomaterials and biological systems. Finally, biomaterials are used in microfabricated devices to replicate the physiological microenvironment in studies using so-called "organ-on-chip," "tissue-on-chip" or "disease-on-chip" models, which can reduce the use of animal models with their inherent high cost and ethical issues, and due to the possible use of human cells can increase the translation of research from lab to clinic. This review gives an overview of recent developments at the interface between microfabrication and biomaterials science, and indicates potential future directions that the field may take. In particular, a trend toward increased scale and automation is apparent, allowing both industrial production of micron-scale biomaterials and high-throughput screening of the interaction of diverse materials libraries with cells and bioengineered tissues and organs.

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The tooth on-a-chip: a microphysiologic model system mimicking the biologic interface of the tooth with biomaterials

Abstract: The tooth-on-chip is a novel platform that emulates the interface between dental materials, tooth and the underlying dental pulp.

Cited by 65 publications

References 39 publications

Progress and Challenges in Microengineering the Dental Pulp Vascular Microenvironment

Progress and Challenges in Microengineering the Dental Pulp Vascular Microenvironment

Bone‐on‐a‐Chip: Microfluidic Technologies and Microphysiologic Models of Bone Tissue

Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research

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