The tissue expression pattern of the AtGRP5 regulatory region is controlled by a combination of positive and negative elements

Abstract

The AtGRP5 gene from Arabidopsis thaliana encodes a glycine-rich protein which has a major activity in protoderm-derived cells and is expressed in cells that undergo the first anatomical modifications leading to somatic embryo development. It has been previously demonstrated that its minimum promoter is 316 bp long including the 5'UTR and presents three putative TATA-boxes sequences and several regions that are homologous to previous characterized cis-acting elements. In order to better characterize the AtGRP5 expression and to identify the promoter regions involved in its preferential epidermal expression, in situ hybridization and 5' promoter deletions were employed. In situ hybridization and GUS expression assays indicate that, besides being present during somatic embryogenesis, AtGRP5 is also expressed during the zygotic embryo development. The sequential 5' deletions indicate that multiple negative and positive regulatory elements are present in the AtGRP5 promoter and operate in order to confer its distinct expression pattern. A 44-bp region was shown to be essential for the epidermal expression of this gene in leaves, stems, flowers and fruits, and is also responsible for high activity of the AtGRP5 promoter in zygotic embryos. An element responsible for the phloem expression was also identified in a 35-bp region.

Abiotic stress and genome dynamics: specific genes and transposable elements response to iron excess in rice

“…For the identification of known plant regulatory promoter elements, 1 kbp upstream from transcription start sites of up-regulated genes were analyzed for potential consensus sequences using the 469 CREs experimentally validated in the literature (Mangeon et al 2010 ; Tsutsui et al 2011 ) and in the PLACE Database. We found 338 predicted CREs with a significant occurrence (p ≤ 0.05).…”

A proteomic approach reveals new actors of nodule response to drought in split‐root grown pea plants

“…These three GRPs belong to the class IV of GRPs also known as RNA‐binding GRPs (GR‐RBP‐s) because besides the glycine‐rich domain, they present a RNA‐recognition motif (RRM) (Lorkovic ). GRPs have been associated to a wide range of different functions in plant cells including cell wall structure, plant defense, cell elongation, abiotic stress response or plant flowering and development (Mangeon et al ). It has been determined that the expression of GRPs is regulated by a number of external stimuli including drought (Sachetto‐Martins et al ).…”

The tomato receptor CuRe1 senses a cell wall protein to identify Cuscuta as a pathogen

“…The biological function of the full-length protein SlGRP is unclear and SlGRP may probably play other roles in tomato not related to cellular defense responses and independent of tomato CuRe1. In general, assigned functions of plant GRPs are multifaceted and range from the stabilization of cell walls to hypothesized regulating functions during abiotic and biotic stress reactions 24 , 25 , which makes it difficult to speculate about the role of the respective GRP in tomato or Cuscuta . Future work will have to reveal what the in vivo function of CrGRP for C. reflexa could be.…”