The TIE1 Transcriptional Repressor Links TCP Transcription Factors with TOPLESS/TOPLESS-RELATED Corepressors and Modulates Leaf Development in<i>Arabidopsis</i>

Qing, Tao; Guo, Dongshu; Wei, Baoye; Zhang, Fan; Pang, Changxu; Jiang, Hao; Zhang, Jinzhe; Wei, Tong; Gu, Hongya; Liu, Qu; Qin, Genji

doi:10.1105/tpc.113.109223

Cited by 116 publications

(154 citation statements)

References 57 publications

(117 reference statements)

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“…In this complex, Cyc8 functions as a Tup1 adaptor (Tzamarias and Struhl, 1994). In plants, many transcription factors can bind TPL directly (Causier et al, 2012), but others, as in Cyc8-Tup1, recruit TPL via molecular bridges such as NINJA, JAZ, Aux/IAA, and TIE1, which links TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors to TPL (Tao et al, 2013), including the KIX proteins presented here.…”

Section: Kix8 and Kix9 Are Tpl Adaptor Proteinsmentioning

confidence: 99%

A Repressor Protein Complex Regulates Leaf Growth in Arabidopsis

et al. 2015

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Cell number is an important determinant of final organ size. In the leaf, a large proportion of cells are derived from the stomatal lineage. Meristemoids, which are stem cell-like precursor cells, undergo asymmetric divisions, generating several pavement cells adjacent to the two guard cells. However, the mechanism controlling the asymmetric divisions of these stem cells prior to differentiation is not well understood. Here, we characterized PEAPOD (PPD) proteins, the only transcriptional regulators known to negatively regulate meristemoid division. PPD proteins interact with KIX8 and KIX9, which act as adaptor proteins for the corepressor TOPLESS. D3-type cyclin encoding genes were identified among direct targets of PPD2, being negatively regulated by PPDs and KIX8/9. Accordingly, kix8 kix9 mutants phenocopied PPD loss-of-function producing larger leaves resulting from increased meristemoid amplifying divisions. The identified conserved complex might be specific for leaf growth in the second dimension, since it is not present in Poaceae (grasses), which also lack the developmental program it controls.

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Section: Kix8 and Kix9 Are Tpl Adaptor Proteinsmentioning

confidence: 99%

A Repressor Protein Complex Regulates Leaf Growth in Arabidopsis

et al. 2015

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“…To verify whether NNC1 is a transcriptional repressor, we performed a classical GAL4/UAS-based system assay to study the transcriptional repression of a protein (Tao et al, 2013). As shown in Figure 6C, the GAL4 DNA binding domain (G4DBD) that binds to the six copies of GAL4 UAS can activate the GUS gene expression.…”

Section: Nnc1 Encodes a Transcription Factor That Negatively Regulatementioning

confidence: 99%

Soybean miR172c Targets the Repressive AP2 Transcription Factor NNC1 to Activate ENOD40 Expression and Regulate Nodule Initiation

et al. 2014

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MicroRNAs are noncoding RNAs that act as master regulators to modulate various biological processes by posttranscriptionally repressing their target genes. Repression of their target mRNA(s) can modulate signaling cascades and subsequent cellular events. Recently, a role for miR172 in soybean (Glycine max) nodulation has been described; however, the molecular mechanism through which miR172 acts to regulate nodulation has yet to be explored. Here, we demonstrate that soybean miR172c modulates both rhizobium infection and nodule organogenesis. miR172c was induced in soybean roots inoculated with either compatible Bradyrhizobium japonicum or lipooligosaccharide Nod factor and was highly upregulated during nodule development. Reduced activity and overexpression of miR172c caused dramatic changes in nodule initiation and nodule number. We show that soybean miR172c regulates nodule formation by repressing its target gene, Nodule Number Control1, which encodes a protein that directly targets the promoter of the early nodulin gene, ENOD40. Interestingly, transcriptional levels of miR172c were regulated by both Nod Factor Receptor1α/5α-mediated activation and by autoregulation of nodulation-mediated inhibition. Thus, we established a direct link between miR172c and the Nod factor signaling pathway in addition to adding a new layer to the precise nodulation regulation mechanism of soybean.

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“…The histochemical GUS staining analysis was described previously (Tao et al, 2013). Briefly, tissues of pEXB1-GUS transgenic lines or DR5-GUS plants were immerged in 90% acetone for 30 min on ice and washed by phosphate buffer twice.…”

Section: Staining and Microscopymentioning

confidence: 99%

The WRKY Transcription Factor WRKY71/EXB1 Controls Shoot Branching by Transcriptionally Regulating RAX Genes in Arabidopsis

et al. 2015

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Plant shoot branching is pivotal for developmental plasticity and crop yield. The formation of branch meristems is regulated by several key transcription factors including REGULATOR OF AXILLARY MERISTEMS1 (RAX1), RAX2, and RAX3. However, the regulatory network of shoot branching is still largely unknown. Here, we report the identification of EXCESSIVE BRANCHES1 (EXB1), which affects axillary meristem (AM) initiation and bud activity. Overexpression of EXB1 in the gain-offunction mutant exb1-D leads to severe bushy and dwarf phenotypes, which result from excessive AM initiation and elevated bud activities. EXB1 encodes the WRKY transcription factor WRKY71, which has demonstrated transactivation activities. Disruption of WRKY71/EXB1 by chimeric repressor silencing technology leads to fewer branches, indicating that EXB1 plays important roles in the control of shoot branching. We demonstrate that EXB1 controls AM initiation by positively regulating the transcription of RAX1, RAX2, and RAX3. Disruption of the RAX genes partially rescues the branching phenotype caused by EXB1 overexpression. We further show that EXB1 also regulates auxin homeostasis in control of shoot branching. Our data demonstrate that EXB1 plays pivotal roles in shoot branching by regulating both transcription of RAX genes and auxin pathways.

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The TIE1 Transcriptional Repressor Links TCP Transcription Factors with TOPLESS/TOPLESS-RELATED Corepressors and Modulates Leaf Development inArabidopsis

Cited by 116 publications

References 57 publications

A Repressor Protein Complex Regulates Leaf Growth in Arabidopsis

A Repressor Protein Complex Regulates Leaf Growth in Arabidopsis

Soybean miR172c Targets the Repressive AP2 Transcription Factor NNC1 to Activate ENOD40 Expression and Regulate Nodule Initiation

The WRKY Transcription Factor WRKY71/EXB1 Controls Shoot Branching by Transcriptionally Regulating RAX Genes in Arabidopsis

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