The WRKY Transcription Factor WRKY71/EXB1 Controls Shoot Branching by Transcriptionally Regulating <i>RAX</i> Genes in Arabidopsis

Guo, Dongshu; Zhang, Jinzhe; Wang, Xinlei; Han, Xiaowei; Wei, Baoye; Wang, Jianqiao; Li, Boxun; Yu, Hao; Qing, Huang; Gu, Hong; Liu, Qu; Qin, Genji

doi:10.1105/tpc.15.00829

Cited by 110 publications

(67 citation statements)

References 69 publications

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“…Mesophyll protoplast preparation and transfection were performed according to a previously reported method (Yoo et al, 2007). For transient expression assays in tobacco leaves, leaves of Nicotiana benthamiana were transiently transformed and examined as previously described (Guo et al, 2015). Biological replicates represent the results of three independent assays.…”

Section: Transient Expression Assaymentioning

confidence: 99%

Type-B ARABIDOPSIS RESPONSE REGULATORs Specify the Shoot Stem Cell Niche by Dual Regulation of WUSCHEL

et al. 2017

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ORCID IDs: 0000-0003-3103-8629 (W.J.M.); 0000-0002-3129-5206 (X.S.Z.)Plants are known for their capacity to regenerate the whole body through de novo formation of apical meristems from a mass of proliferating cells named callus. Exogenous cytokinin and auxin determine cell fate for the establishment of the stem cell niche, which is the vital step of shoot regeneration, but the underlying mechanisms remain unclear. Here, we show that type-B ARABIDOPSIS RESPONSE REGULATORs (ARRs), critical components of cytokinin signaling, activate the transcription of WUSCHEL (WUS), which encodes a key regulator for maintaining stem cells. In parallel, type-B ARRs inhibit auxin accumulation by repressing the expression of YUCCAs, which encode a key enzyme for auxin biosynthesis, indirectly promoting WUS induction. Both pathways are essential for de novo regeneration of the shoot stem cell niche. In addition, the dual regulation of type-B ARRs on WUS transcription is required for the maintenance of the shoot apical meristem in planta. Thus, our results reveal a long-standing missing link between cytokinin signaling and WUS regulator, and the findings provide critical information for understanding cell fate specification.

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Section: Transient Expression Assaymentioning

confidence: 99%

Type-B ARABIDOPSIS RESPONSE REGULATORs Specify the Shoot Stem Cell Niche by Dual Regulation of WUSCHEL

et al. 2017

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“…WRKYs are TFs that have been reported to induce dwarfing (Cai et al, 2014;Guo et al, 2015;Li et al, 2016). We screened five dwarfing rootstocks and five nondwarfing rootstocks of apple and detected the expression of 29 WRKY genes that may function in plant development.…”

Section: Researchmentioning

confidence: 99%

“…In transgenic rice, inhibition of OsWRKY78 resulted in a semi-dwarf plant displaying a small-kernel phenotype due to reduced cell length . OsWRKY11 overexpression reduced the height of transgenic rice plants, leading to plants with a dwarf stature (Cai et al, 2014;Guo et al, 2015). The plant WRKY TFs are characterized by the presence of one or two WRKY domains, including the highly conserved WRKYGQK sequence, and a zinc-binding motif (Duan et al, 2007;Zhang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

MdWRKY9 overexpression confers intensive dwarfing in the M26 rootstock of apple by directly inhibiting brassinosteroid synthetase MdDWF4 expression

et al. 2017

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Dwarfing rootstocks enable high-density planting and are therefore highly desirable in modern apple (Malus domestica) production. M26 is a semi-dwarfing rootstock that is used worldwide, but identifying intensive dwarfing rootstock is a major goal of apple breeding programs. Herein, we show that MdWRKY9 mediates dwarfing by directly inhibiting the transcription of the brassinosteroid (BR) rate-limiting synthetase MdDWF4 and reducing BR production. We found that the transcriptional factor MdWRKY9 is highly expressed in all tested dwarfing rootstocks. Transgenic lines of M26 rootstock overexpressing MdWRKY9 exhibit further dwarfing, which resulted from the reduced BR levels and was reversed via exogenous brassinolide treatment. Both an in vivo chromatin immunoprecipitation (ChIP) analysis and an in vitro electrophoretic mobility shift assay (EMSA) indicated that MdWRKY9 binds to the promoter of MdDWF4. Furthermore, MdWRKY9 repressed MdDWF4 expression in stable transgenic apple plants as determined by quantitative PCR. In addition, RNA-interfered expression of MdWRKY9 in transiently transformed apple calli led to a significant increase of MdDWF4, suggesting MdWRKY9 plays a critical role in regulating the expression of MdDWF4. We report a novel dwarfing mechanism in perennial woody plants that involves WRKY-controlled BR production, and present a new dwarfing M26 rootstock for potential applications in apple production.

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“…RAX proteins have additional roles during reproductive development (Keller et al 2006;Müller et al 2006). Recently, gain-of-function of five closely related WRKY transcription factors each promoted axillary meristem initiation; one of these, EX-CESSIVE BRANCHES1 (EXB1/WRKY71), positively regulates RAX1, 2, and 3 transcription (Guo et al 2015).…”

Section: Axillary Meristem Initiationmentioning

confidence: 99%

Plant Inflorescence Architecture: The Formation, Activity, and Fate of Axillary Meristems

Zhu

Wagner

2019

Cold Spring Harb Perspect Biol

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The above-ground plant body in different plant species can have very distinct forms or architectures that arise by recurrent redeployment of a finite set of building blocks-leaves with axillary meristems, stems or branches, and flowers. The unique architectures of plant inflorescences in different plant families and species, on which this review focuses, determine the reproductive success and yield of wild and cultivated plants. Major contributors to the inflorescence architecture are the activity and developmental trajectories adopted by axillary meristems, which determine the degree of branching and the number of flowers formed. Recent advances in genetic and molecular analyses in diverse flowering plants have uncovered both common regulatory principles and unique players and/or regulatory interactions that underlie inflorescence architecture. Modulating activity of these regulators has already led to yield increases in the field. Additional insight into the underlying regulatory interactions and principles will not only uncover how their rewiring resulted in altered plant form, but will also enhance efforts at optimizing plant architecture in desirable ways in crop species.

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The WRKY Transcription Factor WRKY71/EXB1 Controls Shoot Branching by Transcriptionally Regulating RAX Genes in Arabidopsis

Cited by 110 publications

References 69 publications

Type-B ARABIDOPSIS RESPONSE REGULATORs Specify the Shoot Stem Cell Niche by Dual Regulation of WUSCHEL

Type-B ARABIDOPSIS RESPONSE REGULATORs Specify the Shoot Stem Cell Niche by Dual Regulation of WUSCHEL

MdWRKY9 overexpression confers intensive dwarfing in the M26 rootstock of apple by directly inhibiting brassinosteroid synthetase MdDWF4 expression

Plant Inflorescence Architecture: The Formation, Activity, and Fate of Axillary Meristems

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