The therapeutic effect of Bletilla striata extracts on LPS-induced acute lung injury by regulation of inflammation and oxidation

Wang, Yongjie; Huang, Weizhen; Zhang, Jiaozhen; Yang, Min; Qi, Qiuchen; Wang, Kaiming; Li, Ang; Zhao, Zhongxi

doi:10.1039/c6ra17059e

Cited by 12 publications

(9 citation statements)

References 46 publications

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“…Treatment with BG@BSA NPs reduced MDA and increased SOD in the lung. Similar results have been documented using BM 60 [25].…”

Section: Antioxidant Activitysupporting

confidence: 89%

“…However, the lung wet/dry ratios were greatly decreased in animal treated with BG@BSA NPs, thus con rming the protective effect of BG@BSA NPs. BM60 decreased the lung wet-to-dry ratio, which suggested the protective effect on LPS-induced ALI [25].…”

Section: Effect Of Bg@bsa Nps On Cell Viability and No Productionmentioning

confidence: 81%

“…11b-d). Similarly, the in ammatory cells and TNF-a, IL-1b, and IL-6 production were greatly inhibited by BM60 [25].…”

Section: Mpo and In Ammatory Cytokine Activitymentioning

confidence: 85%

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Novel Combined Preparation and Investigation of Bergenin Loaded Albumin Nanoparticles for the Treatment and Care of Acute Lung Injury: In Vitro and In Vivo Evaluations

Yan

Chen

Xie

2021

Preprint

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A new method for targeting lung infections is of great interest using biodegradable nanoparticles. In this study, bergenin loaded BSA NPs were developed against lung injury. Briefly, BG@BSA NPS were synthesized and characterized. HPLC recorded the major peak of bergenin. UV-vis spectra had an absorbance at 376nm. XRD revealed the presence of crystalline particles. FTIR confirmed the occurrence of functionalized molecules in the synthesized NPS. The particles were highly stable with a net negative charge of -24.2. The morphology of NPS were determined by SEM and TEM. The mean particle size was 124.26nm. The production of NO by NR8383 cells was decreased by BG@BSA NPs. Also, in mice, lipopolysaccharide mediated acute lung inflammation was induced. BG@BSA NPs reduced macrophages and neutrophils in BALF and remarkably enhanced wet-to-dry weight (W/D) ratios and myeloperoxidase (MPO) activity. Further, BG@BSA NPs inhibited the production of inflammatory cells as well as tumour necrosis factor. The histopathological studies revealed that the damage and neutrophil infiltration was greatly inhibited by BG@BSA NPs. This indicates that BG@BSA NPs may be used to treat lung infections Therefore, this study has given new insight into producing an active drug for the treatment of lung associated diseases in the future.

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“…Treatment with BG@BSA NPs reduced MDA and increased SOD in the lung. Similar results have been documented using BM 60 [25].…”

Section: Antioxidant Activitysupporting

confidence: 89%

Section: Effect Of Bg@bsa Nps On Cell Viability and No Productionmentioning

confidence: 81%

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Novel Combined Preparation and Investigation of Bergenin Loaded Albumin Nanoparticles for the Treatment and Care of Acute Lung Injury: In Vitro and In Vivo Evaluations

Yan

Chen

Xie

2021

Preprint

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“…We found that Wang et al [43] also reported an antiinflammatory, antioxidant system regulating an active part of Bletilla striata. Briefly, the 95% ethanol extract of Bletilla striata was fractionated by systematic solvent; then, the ethyl acetate fraction was eluted by gradient ethanol on MCI column chromatography, combined with total phenol content and DPPH free radical scavenging activity screening; an active fraction BM60 was obtained and characterized by HPLC-ESI-HRMS.…”

Section: Discussionsupporting

confidence: 53%

Anti-Inflammatory Effect Fraction of Bletilla striata and Its Protective Effect on LPS-Induced Acute Lung Injury

Zhang

Ning

Pan

et al. 2021

Mediators of Inflammation

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Bletilla striata is a well-known traditional Chinese herb with anti-inflammatory properties that is widely used in the treatment of lung conditions such as silicosis, tuberculosis, and pneumogastric hemorrhage. However, little information on the anti-inflammatory ingredients and their activities is available. In this study, an effect fraction of Bletilla striata (EFBS) was enriched, and its anti-inflammatory activities and underlying mechanisms were investigated. EFBS was enriched by polyamide column chromatography and characterized by HPLC; an LPS-induced acute lung injury model was used to evaluate the anti-inflammatory activities of EFBS. Meanwhile, the main anti-inflammation-contributing ingredients and possible molecular mechanism of anti-inflammatory activity in EFBS were verified by component-knockout method combined with LPS-induced RAW264.7 cell model. The EFBS mainly consisted of coelonin (15.88%), batatasin III (32.49%), 3 ′ -O-methylbatatasin III (6.96%), and 3-hydroxy-5-methoxy bibenzyl (2.51%). Pretreatment with the EFBS (20 mg/kg and 60 mg/kg) for five days prior to the administration of LPS resulted in decreases in wet-to-dry lung weight ratio, neutrophil number, MPO activity, total protein concentration, NO level, and MDA level, as well as IL-1β, IL-6, MCP-1, and TNF-α concentrations in the bronchoalveolar lavage fluid. Western blot analysis demonstrated the increased expressions of iNOS, COX-2, and NF-κB p65 in the LPS treatment group, all of which were ameliorated by EFBS pretreatment. Histological examination confirmed the protective effect of the EFBS. Additionally, component-knockout assay confirmed that these four quantitative components contributed significantly to the anti-inflammatory effect of EFBS. Coelonin, batatasin III, 3 ′ -O-methylbatatasin III and 3-hydroxy-5-methoxy bibenzyl were the main anti-inflammatory components of EFBS and could regulate the expression of downstream inflammatory cytokines by inhibiting p65 nuclear translocation. These findings uncover, in part, the molecular basis underlying the anti-inflammatory activity of Bletilla striata.

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“…As the synthesis of MIPs is also relatively easy and cheap, they are a preferred substitute for natural receptors and have been widely used in the elds of chromatography, solid phase extraction, chemical sensors, etc. 24,25 However, with free radical polymerization methods, the rate of chain growth cannot be controlled, and side reactions during chain transfer and chain termination cause substantial variation in the size of the polymer, 26 making it difficult to prepare conventional MIPs. Living/ controlled free radical polymerization methods such as surfaceinitiated atom transfer radical polymerization (SI-ATRP) can produce more uniform polymers by controlling the rate of chain growth.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of monodisperse magnetic restricted microspheres for recognition of thiamphenicol in milk

et al. 2021

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The therapeutic effect of Bletilla striata extracts on LPS-induced acute lung injury by regulation of inflammation and oxidation

Abstract: Identification of active ingredients, and their structures, from Bletilla striata and investigation of the antioxidative and anti-inflammatory responses in vitro and in vivo.

Cited by 12 publications

References 46 publications

Novel Combined Preparation and Investigation of Bergenin Loaded Albumin Nanoparticles for the Treatment and Care of Acute Lung Injury: In Vitro and In Vivo Evaluations

Novel Combined Preparation and Investigation of Bergenin Loaded Albumin Nanoparticles for the Treatment and Care of Acute Lung Injury: In Vitro and In Vivo Evaluations

Anti-Inflammatory Effect Fraction of Bletilla striata and Its Protective Effect on LPS-Induced Acute Lung Injury

Synthesis of monodisperse magnetic restricted microspheres for recognition of thiamphenicol in milk

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