The teleost polymeric Ig receptor counterpart in ballan wrasse (Labrus bergylta) differs from pIgR in higher vertebrates

Etayo, Angela; Bjørgen, Håvard; Koppang, Erling Olaf; Hordvik, Ivar

doi:10.1016/j.vetimm.2022.110440

Cited by 10 publications

(3 citation statements)

References 58 publications

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“…Next, we quantified the expression of pIgRL3.5 and pIgRL4.2 in all tissues of adult zebrafish and found that their highest expression levels occurred in the gut or liver, which is consistent with the expression patterns of pIgR or pIgRL genes in previous studies in sea bass, crucian carp (Carassius auratus ), ballan wrasse (Labrus bergylta ), and dojo loach (Misgurnus anguillicaudatus ) [26][27][28][29] . These results confirmed that pIgRL3.5 and pIgRL4.2 were mainly expressed in the zebrafish intestinal mucosa, suggesting their roles in mucosal immunity.…”

Section: Discussionsupporting

confidence: 86%

Identification of two pIgR-like molecules in teleost fish with opposite roles in mucosal immunity against bacterial infection

xu,

Xu,

Wang

et al. 2024

Preprint

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The polymeric immunoglobulin (Ig) receptor-like (pIgRL) molecules have been identified in teleost fish. However, compare to the functional studies of their related genes (mammalian CD300 family, et al) in eliminating pathogen invasion while preserving homeostasis, the roles of pIgRL in teleost fish remain unclear. In this study, we demonstrated that a pair of pIgRL molecules in zebrafish, pIgRL3.5 and pIgRL4.2, were highly expressed in the intestine and immune cells. Moreover, we constructed an Edwardsiella piscicida infection model, which induced strong inflammatory responses in the zebrafish intestine. Interestingly, pIgRL3.5 and pIgRL4.2 exhibited opposite inducible expression patterns in response to bacterial infection, suggesting that they perform different roles. More importantly, by conducting overexpression and knockdown experiments, our findings demonstrated that zebrafish pIgRL3.5 played a protective role in the host defense against E. piscicida infection by inhibiting excessive inflammatory responses. In contrast, pIgRL4.2 facilitated pathogen growth and dissemination in zebrafish intestine. Collectively, our findings were the first to demonstrate that a pair of pIgRL molecules in teleost fish play opposite roles in mucosal immune response to bacterial infection. Therefore, our results provide crucial insights into the conserved role of pIgRL molecules in immune regulatory functions throughout vertebrate evolution.

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Section: Discussionsupporting

confidence: 86%

Identification of two pIgR-like molecules in teleost fish with opposite roles in mucosal immunity against bacterial infection

xu,

Xu,

Wang

et al. 2024

Preprint

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“…The polymeric immunoglobulin receptor (pIgR) is a key player during mucosal immune responses [22]. In fish, pIgR can bind to IgT and/or IgM [23]. However, little is known about pIgR-mediated immune excretion.…”

Section: Introductionmentioning

confidence: 99%

The Distribution of IgT mRNA+ Cells in the Gut of the Atlantic Salmon (Salmo salar L.)

Castro,

Barac,

Hansen

et al. 2023

Animals

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The newly discovered IgT+ B cell is thought to play a dominant role in mucosal immunity, but limited studies have examined its distribution in fish species, hindering our understanding of its function. This study investigated IgT and poly Ig receptor (pIgR) mRNA+ cell distribution in Atlantic salmon (Salmo salar) gut using RNAscope in situ hybridization (ISH) and assessed the effects of vaccination. The pyloric caeca, mid-intestine (first and second parts), and posterior segment in two weight stages (Group 1: avg. 153 g, Group 2: avg. 1717 g) were examined in both vaccinated and unvaccinated fish. ISH revealed more IgT mRNA+ cells in the second part of the midgut compared to other intestinal segments, as well as a higher number of positive cells in Group 2 (older fish). In line with previous findings, intraperitoneal vaccination had no significant impact on the number of IgT+ transcripts. IgT mRNA+ cells were found mostly in the lamina propria and near capillaries, while pIgR was registered in both the lamina propria and mucosa. Interestingly, vaccinated fish presented adhesions and granulomatous tissue in the peritoneum, with both IgT and pIgR mRNA+ cells. Taken together, these results suggest that the distribution of IgT mRNA+ cells in the intestine of Atlantic salmon is region-specific and is not affected by intraperitoneal vaccination but varies with fish age.

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“…Differently from mammals, Igs in teleost only four types (IgM, IgD, IgZ/IgT, IgM-IgZ chimera) [12], of which IgM was discovered firstly [13]. To date, polymeric immunoglobulin receptor (pIgR), one of the FcRs, in grass carp (Ctenopharyngodon idellus) [14], flounder (Paralichthys olivaceus) [15], Nile tilapia (Oreochromis niloticus) [16], ballan wrasse (Labrus bergylta) [17] and largemouth bass (Micropterus salmoides) [18] have been reported that could bind to IgM. However, regarding classical IgM-binding FcRs homologs, it has only been identified in FcγRI of ayu (Plecoglossus altivelis) [19] and FcRI of channel catfish (Ictalurus punctatus) [2].…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of Largemouth Bass (Micropterus salmoides) Soluble FcγR Homolog in Response to Bacterial Infection

Nie

et al. 2022

IJMS

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Fc receptors (FcRs) are key players in antibody-dependent cellular phagocytosis (ADCP) with their specific recognition of the Fc portion of an immunoglobulin. Despite reports of FcγR-mediated phagocytosis in mammals, little is known about the effects of soluble FcγRs on the immune response. In this study, FcγRIα was cloned from the largemouth bass (Micropterus salmoides) (MsFcγRIα). Without a transmembrane segment or a cytoplasmic tail, MsFcγRIα was identified as a soluble form protein and widely distributed in the spleen, head kidney, and intestine. The native MsFcγRIα was detected in the serum of Nocardia seriolae-infected largemouth bass and the supernatants of transfected HEK293 cells. Additionally, it was verified that the transfected cells’ surface secreted MsFcRIα could bind to largemouth bass IgM. Moreover, the expression changes of MsFcγRIα, Syk, and Lyn indicated that MsFcγRIα was engaged in the acute phase response to bacteria, and the FcγR-mediated phagocytosis pathway was activated by Nocardia seriolae stimulation. Furthermore, recombinant MsFcγRIα could enhance both reactive oxygen species (ROS) and phagocytosis to Nocardia seriolae of leukocytes, presumably through the interaction of MsFcγRIα with a complement receptor. In conclusion, these findings provided a better understanding of the function of soluble FcγRs in the immune response and further shed light on the mechanism of phagocytosis in teleosts.

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The teleost polymeric Ig receptor counterpart in ballan wrasse (Labrus bergylta) differs from pIgR in higher vertebrates

Cited by 10 publications

References 58 publications

Identification of two pIgR-like molecules in teleost fish with opposite roles in mucosal immunity against bacterial infection

Identification of two pIgR-like molecules in teleost fish with opposite roles in mucosal immunity against bacterial infection

The Distribution of IgT mRNA+ Cells in the Gut of the Atlantic Salmon (Salmo salar L.)

Functional Characterization of Largemouth Bass (Micropterus salmoides) Soluble FcγR Homolog in Response to Bacterial Infection

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