The taxonomy of three species of microsporidia (Protozoa: Microspora) from an oakwood population of winter moths Operophtera brumata (L.) (Lepidoptera: Geometridae)

Morphology, molecular characteristics and prevalence of a Cystosporogenes species (Microsporidia) isolated from Agrilus anxius (Coleoptera: Buprestidae)

“…In contrast, species of the genus Endoreticulatus develop within a parasitophorous membrane derived from host endoplasmic reticulum (Brooks et al, 1988). Mature spores observed in the present study shared several structural characteristics with previously described Cystosporogenes isolates as did gross pathology, life cycle stages and ultrastructural characteristics (Canning et al, 1983;Kleespies et al, 2003;van Frankenhuyzen et al, 2004).…”

Occurrence of Cystosporogenes sp. (Protozoa, Microsporidia) in a multi-species insect production facility and its elimination from a colony of the eastern spruce budworm, Choristoneura fumiferana (Clem.) (Lepidoptera: Tortricidae)

“…Infection by Endoreticulatus is typically confined to midgut epithelial cells (Brooks et al, 1988;Milner and Briese, 1986) and is therefore not transmitted vertically (Wilson and Kaupp, 1984). Canning et al (1983) report that C. operophterae primarily infects the silk gland but that infection also spreads to gut epithelial cells, Malpighian tubules, fat body, muscles, and oocytes. Kleespies et al (2003) identified the midgut epithelium as the primary infection site of C. legeri with the infection readily spreading to other organs and tissues, such as fat body, ganglia, gonads, and eggs.…”

“…The genus Cystosporogenes was established by Canning et al (1985) to accommodate Pleistophora operophterae, a species that was described from winter moth, Operophtera brumata (L.) (Canning et al, 1983). It was transferred to the new genus based on ultrastructural criteria, the most prominent one being that all stages develop inside a vesicle that is bounded by a single membrane of uncertain origin.…”

Further observations on the ultrastructure of Cystosporogenes operophterae (Canning, 1960) (phylum Microsporidia) parasitic in Operophtera brumata L. (Lepidoptera, Geometridae)

“…Light microscopy of C. operophterae (Canning et al, 1983) and E. fidelis (Brooks et al, 1988) revealed large multinucleate plasmodia in the sporogonic phase undergoing multiple fission. In contrast, electron microscopy revealed several rather small, rounded or elongate sporogonic stages, lying separately in the vacuoles with a fibrous matrix between them (Brooks et al, 1988;Canning et al, 1985;this paper).…”

“…The generic name was subsequently changed to Pleistophora by Sprague (1977) and later to Cystosporogenes by Canning et al (1985). The species infects most tissues of the winter moth Operophtera brumata L. including the salivary glands where the infected cells are mildly hypertrophied and distributed in a regular pattern throughout the glands (Canning et al, 1983). Cystosporogenes is one of several genera in which sporogony culminates in numerous spores retained within an envelope.…”