The Targeted Antitumor Effects of C- PC/CMC-CD59sp Nanoparticles on HeLa Cells in Vitro and in Vivo

Abstract

The novel C-PC/CMC-CD59sp-NPs were made by carbocymethyl chitosan (CMC) loading C-phycocyanin (C-PC) with the lead of CD59 specific ligand peptide (CD59sp) for targeting, and the characteristics and targeted anti-tumor mechanism were explored in order to realize the targeted therapy of C-PC on the growth of HeLa cells both in vitro and vivo. The targeting nanoparticles were synthesized by ionic-gelation method, and the optimal condition was selected out by orthogonal analysis. The properties of nanoparticles were observed by laser particle analyzer and dynamic light scattering (DLS) and Fourier Transform Infrared Spectrometer (FTIR). The effects of nanoparticles on the proliferation of HeLa cells in vitro were assessed by MTT assay. The mice model with tumor was constructed by subcutaneous injection of HeLa cells into the left axilla of NU/NU mice. The weight of tumor and the spleen were tested. The expression quantities of cleaved caspase-3, Bcl-2 were determined by western blot and immunofluorescent staining. Results showed the morphology of the finally prepared nanoparticles was well distributed with a diameter distribution of 200±11.3 nm and zeta potential of -19.5±4.12mV. Under the guidance of CD59sp, the targeting nanoparticles could targetedly and efficiently arrive at the surface of HeLa cells, and had obvious inhibitory effect on HeLa cells proliferation both in vitro and vivo. Moreover, the nanoparticles could induce cell apoptosis by up-regulation of cleaved caspase-3 proteins expression, but down-regulation of Bcl-2 and cyclinD1 proteins. Our study provided a new idea for the research and development of marine drugs, and supplied a theoretical support for the target therapy of anticancer drug.

CD59 receptor targeted delivery of miRNA-1284 and cisplatin-loaded liposomes for effective therapeutic efficacy against cervical cancer cells

“…A significant shift in the fluorescence histogram is proof of its enhanced cellular accumulations in the HeLa cells. These results were consistent with the previous reports of higher cellular uptake and higher therapeutic efficacy with CD59-tagreted carrier system in the cancer cells (Wang et al 2017).…”

CD59 receptor targeted delivery of miRNA-1284 and Cisplatin-loaded liposomes for effective therapeutic efficacy against cervical cancer cells

“…These results were consistent with the previous reports of higher cellular uptake and higher therapeutic efficacy with CD59-tagreted carrier system in the cancer cells (Wang et al, 2017).…”

Recent Progress of Marine Polypeptides as Anticancer Agents

“…However, overexpression of Akt in cancer cells leads to high susceptibility to Iturin A-induced apoptosis. Iturin A inhibited tumor growth with reduced expressions of Ki-67, P-MAPK, P-Akt, CD-31, P-FoxO3a and P-GSK3 in a xenograft model of breast cancer [39]. Iturin F 1 , iturin F 2 , iturin A 8 and iturin A 9 were separated from Bacillus sp.…”

“…C-PC/CMC-CD59sp nanoparticles can inhibit the growth of HeLa cells in vitro and tumors in vivo. Furthermore, the nanoparticles induce cellular apoptosis through downregulating Bcl-2 and cyclin D1 proteins [39].…”