The Tape-Stripping Technique as a Method for Drug Quantification in Skin

Escobar-Chávez, José Juan; Merino-Sanjuán, V.; López-Cervantes, Miriam; Urbán-Morlán, Zaida; Piñón-Segundo, Elizabeth; Quintanar‐Guerrero, David; Ganem-Rondero, Adriana

doi:10.18433/j3201z

Cited by 219 publications

(180 citation statements)

References 117 publications

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“…Tape stripping is a simple and effective method for removing the SC (Figure 1) and is commonly employed during in vivo studies investigating the percutaneous penetration and disposition of topically applied candidate drugs as well as in investigations of drugs intended to restore damaged epithelial barriers (Escobar-Chavez et al 2008). One study was performed with the objective to assess the remaining thickness of the SC following 0, 10, 20, 30, 40, and 50 repetitions of tape stripping of skin on 3 young adult male Yucatan miniature swine weighing 33-36 kg each.…”

Section: Skin Stripping For Dermal Penetrationmentioning

confidence: 99%

The Miniature Swine as a Model in Experimental and Translational Medicine

2016

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The use of the miniature swine as a nonrodent species in research has continued to expand for over a decade, and they are becoming routinely used both in experimental pharmacology and as a therapeutic model for human diseases. Miniature swine models are regularly used for studies designed to assess efficacy and safety of new therapeutic compounds given through different routes of exposure and are used as an alternative model to rodents, canines, or nonhuman primates. Translational preclinical swine study data presented here support the current understanding that miniature swine are the animal model of choice for the assessment of drugs targeting endocrine, dermal, and ocular disorders. Because research investigators need to be familiar with some of the important features of the models developed in the miniature swine in order to place clinical and experimental findings in their proper perspective, relevant references and data from these models will be presented, compared, and partially illustrated.

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Section: Skin Stripping For Dermal Penetrationmentioning

confidence: 99%

The Miniature Swine as a Model in Experimental and Translational Medicine

2016

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“…This technique in its standardized form is well suited to determine the DPK of topical formulations, where the drug concentration is determined in the SC layers, which are progressively removed by tape stripping. This method is a simple, efficient, quick and relatively non-invasive approach to assess the quality and efficacy of drugs, excipients, cosmetics in the skin following the topical dermatological application (Escobar-Chávez et al, 2008). Indeed, the drug or excipients level in the skin can be determined either in the removed tape strips, or directly in the tape stripped skin.…”

Section: Tape Stripping Techniquementioning

confidence: 99%

Basic considerations in the dermatokinetics of topical formulations

Nair

Jacob

Al‐Dhubiab

et al. 2013

Braz. J. Pharm. Sci.

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Assessing the bioavailability of drug molecules at the site of action provides better insight into the efficiency of a dosage form. However, determining drug concentration in the skin layers following topical application of dermatological formulations is a great challenge. The protocols followed in oral formulations could not be applied for topical dosage forms. The regulatory agencies are considering several possible approaches such as tape stripping, microdialysis etc. On the other hand, the skin bioavailability assessment of xenobiotics is equally important for topical formulations in order to evaluate the toxicity. It is always possible that drug molecules applied on the skin surface may transport thorough the skin and reaches systemic circulation. Thus the real time measurement of molecules in the skin layer has become obligatory. In the last two decades, quite a few investigations have been carried out to assess the skin bioavailability and toxicity of topical/dermatological products. This review provides current understanding on the basics of dermatokinetics, drug depot formation, skin metabolism and clearance of drug molecules from the skin layers following application of topical formulations.Uniterms: Dermatological formulations/pharmacokinetics. Dermatological formulations/ toxicity. Dermatokinetics. Skin/metabolism. Microdialysis. Tape stripping.A avaliação da biodisponibilidade de moléculas de fármacos no sítio de ação oferece melhor compreensão sobre a eficiência da forma de dosagem. Entretanto, a determinação da concentração de fármaco nas camadas da pele em seguida à aplicação tópica de formulações dermatológicas é um grande desafio. Os protocolos seguidos para as formulações orais não podem ser aplicados para as formulações tópicas. As agências regulatórias consideram várias abordagens possíveis, tape stripping, microdiálise etc. Por outro lado, a avaliação da biodisponibilidade de xenobióticos na pele é igualmente importante para as formulações tópicas para se avaliar a toxicidade. É sempre possível que as moléculas de fármaco aplicadas na superfície da pele sejam transportadas através da pele e alcancem a circulação sistêmica. Assim, a medida em tempo real de moléculas na camada da pele tem se tornado obrigatória. Nas últimas duas décadas, realizaram-se poucas pesquisas para avaliar a biodisponibilidade da pele e a toxicidade de produtos tópicos/dermatológicos. Esta revisão fornece a compreensão atual com base na dermatocinética, formação de fármaco de depósito, metabolismo da pele e o clearance das moléculas de fármaco das camadas da pele em seguida à aplicação de formulações tópicas.Unitermos: Formulações dermatológicas/farmacocinética. Formulações dermatológicas/toxicidade. Dermatocinética Pele/metabolismo. Microdiálise. Tape stripping.

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“…This method is based on removing of stratum corneum layers of the skin. The amount of removed stratum corneum is not constant and it depends on many parameters such as cohesion between cells, hydration or body sites [40]. This technique has been used for evaluation of the barrier function of skin, i.e.…”

Section: Diagnostics Of Barrier Properties Of the Skin-tewl (Transepimentioning

confidence: 99%

Enhancement of Percutaneous Absorption on Skin by Plasma Drug Delivery Method

Shimizu¹,

Krištof²

2017

Advanced Technology for Delivering Therapeutics

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Transdermal drug delivery (TDD) is a painless method of low-dose drug delivery. The advantages and disadvantages of transdermal drug delivery methods are named and basic methods such as using chemical enhancers, iontophoresis and electrophoresis are introduced. One of the promising methods make use of plasma which is generated in atmospheric pressure mostly in volume or on surface dielectric barrier discharge (DBD) or in plasma jet. As the plasma produces various particles according to the used gas, UV radiation and heat, their effects on skin and barrier function are described. Improvement of transdermal drug delivery of hydrophilic drug galantamine hydrobromide (GaHBr) using microplasma electrode is introduced.

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The Tape-Stripping Technique as a Method for Drug Quantification in Skin

Cited by 219 publications

References 117 publications

The Miniature Swine as a Model in Experimental and Translational Medicine

The Miniature Swine as a Model in Experimental and Translational Medicine

Basic considerations in the dermatokinetics of topical formulations

Enhancement of Percutaneous Absorption on Skin by Plasma Drug Delivery Method

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