The Synthesis of Some Potentiallly Blood Compatible Heparin-like Polymeric Biomaterials

Gebelein, Charles G.; Murphy, Daniel B.

doi:10.1007/978-1-4613-1829-3_25

Cited by 7 publications

(4 citation statements)

References 20 publications

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“…Heparin is a natural polyanionic polysaccharide carrying sulfonate groups ( , ). Therefore, several investigations using sulfonated polymers have been performed ( − ). Recently, it has been found that sulfated hyaluronic acid has a heparin-like property ( − ).…”

Section: Introductionmentioning

confidence: 99%

Photoimmobilization of Sulfated Hyaluronic Acid for Antithrombogenicity

et al. 1997

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Anticoagulant polymer sulfated hyaluronic acid was patterned immobilized on a poly(ethylene terephthalate) (PET) film in a specific pattern by photolithography. Hyaluronic acid was sulfated by a sulfur trioxide-pyridine complex. The polymer was coupled with azidoaniline. The derivatized polymer was cast on a PET film from aqueous solution. After drying, the film was photoirradiated in the presence or absence of a photomask. The micropatterning was confirmed by staining with a dye, brilliant green. Since the anticoagulant polymer has negative charges, the cationic dye was adsorbed on the regions where the anticoagulant polymer was immobilized. Platelet adhesion was reduced on the sulfated hyaluronic acid-immobilized areas. The immobilized sulfated hyaluronic acid significantly reduced thrombus formation.

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Section: Introductionmentioning

confidence: 99%

Photoimmobilization of Sulfated Hyaluronic Acid for Antithrombogenicity

et al. 1997

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“…The polyelectrolyte samples were prepared by a postpolymerization functionalization reaction on polyisoprene (PI) homopolymer precursors. The polyisoprene precursors were synthesized by living anionic polymerization high-vacuum techniques, which provide controlled molecular characteristics and low polydispersities. , Consequently, the precursors were functionalized by reaction of the isoprene double bonds with chlorosulfonyl isocyanate (CSI, from Acros), followed by basic hydrolysis of the lactam intermediate with NaOH. , Thus, sodium sulfamate and carboxylate groups are introduced in the same isoprene monomeric unit. The resulting anionic polyelectrolyte is characterized by high charge density and intriguing pH sensitivity, since it carries both strongly charged pH independent SO 3 − groups and weak acidic COO − groups, which are neutralized at low pH values.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, the particular synthetic polyelectrolyte can be regarded as a must simpler analogue that would allow for comparisons with the behavior of proteins. Furthermore, the structural resemblance of SCPI to the natural polysaccharide heparin, as far as the functional polar groups are concerned, enables possible use in biomedical applications, that is, as an anticoagulant agent. , All these features give strong motivation to study the behavior of SCPI in aqueous solutions. To gain information on the effects of macromolecular structure and conformation and those of electrostatic and hydrophobic interactions in polyelectrolyte solutions, the solution behavior of SCPI was investigated as a function of the molecular weight and the concentration of the polyelectrolyte and the ionic strength, pH, and temperature of the solution.…”

Section: Introductionmentioning

confidence: 99%

Solution Behavior of Poly(sodium(sulfamate-carboxylate)isoprene), a pH Sensitive and Intrinsically Hydrophobic Polyelectrolyte

2010

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The solution properties of a novel pH sensitive and intrinsically hydrophobic polyelectrolyte poly(sodium(sulfamate-carboxylate)isoprene) (SCPI) were investigated by means of dynamic, static, and electrophoretic light-scattering and fluorescence spectroscopy techniques. Because of the pH dependent charge density of the polyelectrolyte chain, the dynamics and structure of the solution were studied at both pH 7 (high charge density) and pH 3 (low charge density) and at low ionic strength conditions for two samples of different molecular weights. In all cases, a fast and a slow diffusive mode were observed. The fast mode is attributed to the diffusion of isolated polyelectrolyte chains, while the slow mode denotes the presence of multichain domains in solution, formed by electrostatic and/or hydrophobic interactions. The size and density of the multichain domains were reduced with decreasing charge density and molecular weight. Effective charge of the particles does not depend appreciably on solution pH, while fluorescence spectroscopy revealed the presence of hydrophobic domains in the studied systems. It was found that increasing temperature resulted in the compaction of both isolated chains and multichain domains due to the hydrophobic effect. Furthermore the increase of the ionic strength of the solution led to a partial dissociation of the multichain domains at short times and at pH 7, while it led to increased aggregation and precipitation at pH 3 and long times. The performed experiments allowed for a separation of the electrostatic and hydrophobic contributions to the self-assembly of the particular polyelectrolyte systems.

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“…Although materials with truly blood compatibility have not been found, material modification is a very efficient method in improving blood compatibility [8][9][10]. To improve the blood compatibility of biomaterials, more attention was paid to heparin-like materials [11][12][13][14][15]. Heparin-like material exhibited good blood compatibility like heparin molecules, and the research about the anticoagulant mechanism of heparin-like polymers help to understand the interactive relationship between materials and blood at the molecular level and the relationship between the structure of materials and their anticoagulant activity [16].…”

Section: Introductionmentioning

confidence: 99%