The Synthesis and Stability Study of Silver Nanoparticles Prepared by Using <i>p</i>-Aminobenzoic Acid as Reducing and Stabilizing Agent

Abstract

A study to examine the performance of p-aminobenzoic acid as both reducing agent for silver nitrate to silver nanoparticles (AgNPs) and stabilizing agent for the formed AgNPs has been done. The synthesis of AgNPs was performed by mixing silver nitrate solution as precursor with p-aminobenzoic acid solution and heating it in a boiling water bath. After the solution turned to yellow, the reaction stopped by cooling it in tap water. The formed AgNPs were analyzed by using UV-Vis spectrophotometry to evaluate their SPR absorption in wavelength range of 400–500 nm. The synthesis process was highly depend on the pH, reaction time, and mole ratios of the reactants. The synthesis only occur in pH 11 and at reaction time 30 min, the particle size of the formed AgNPs was 12 ± 7 nm. Longer reaction time increased the reducing performance of p-aminobenzoic acid in AgNPs synthesis but decreased its stabilizing performance. The increase of silver nitrate amount relative to p-aminobenzoic acid in the synthesis increased the reducing and stabilizing performance of p-aminobenzoic acid and the optimum mole ratio between AgNO3 and p-aminobenzoic acid was 5:100 (AgNO3 to p-aminobenzoic acid).

The Performance of p-Aminosalicylic Acid As Reducing and Stabilizing Agent in Silver Nanoparticles Synthesis

“…The same optimum pH was also obtained for several reducing agents, which are the derivatives of the benzoic acid, such as hydroxybenzoic acid 16,17 , dihydroxybenzoi acid 18 , and aminobenzoic acid 21 . The synthesis of AgNPs by using derivatives of benzoic acid is usually conducted under basic conditions.…”

Effect of Reaction Time and Stability Properties of Gold Nanoparticles Synthesized by <i>p</i>-Aminobenzoic Acid and <i>p</i>-Aminosalicylic Acid

“…It is predicted that the longer the solution is heated, the molecules in the solution would still be active, even when the heating process has stopped. The active molecules will react further so the reduction process would still occur even when the heating has been stopped [20].…”

Antibacterial Activity of Silver Nanoparticles Capped by <i>p</i>-Aminobenzoic Acid on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>

“…The synthesis of AgNPs was performed according to the previous study [11] with some modification. Briefly, p-aminobenzoic acid (Mr = 137.14 g mol -1 ) was weighed and dissolved in distilled water.…”

“…Synthesis of AgNPs was performed by reacting 5 × 10 -3 mol L -1 p-aminobenzoic acid and silver nitrate at various concentrations, ranging from 0 to 1.5 × 10 -3 mol L -1 . The synthesis was performed in the optimum condition based on the previous study [11]. The pH was 11 because the reaction can only take place in this condition.…”

“…The synthesis of AgNPs using p-aminobenzoic acid as a reducing and stabilizing agent was found to be environmentally friendly because of the use of a non-toxic chemical as a reducing and stabilizing agent and because the reaction happened in a short time. The synthesized AgNPs also had excellent stability for more than 16 weeks [11]. In this study, the antibacterial activity of AgNPs which were synthesized using p-aminobenzoic acid was evaluated.…”