The Synergistic Effect of Zinc Ferrite Nanoparticles Uniformly Deposited on Silver Nanowires for the Biofilm Inhibition of Candida albicans

Abstract

Near-monodisperse zinc ferrite nanoparticles (ZnFe2O4 NPs) are synthesized by a co-precipitation method and deposited on the surface of silver nanowires (AgNWs), employing a stepwise solution method. The resulting hybrid nanostructures (ZnFe2O4@AgNWs) show a thin and uniform layer of ZnFe2O4 NPs at an optimum weight ratio of 1:6 between the two component nanostructures. The hybrid nanostructures retain the high crystal quality and phase purity of their constituents. It is demonstrated that the ZnFe2O4@AgNWs hybrid nanostructures are effective at inhibiting the biofilm formation of Candida albicans cells. The biofilm inhibition activity of the hybrid nanostructures is estimated to be more than 50% at a low concentration of 100 µg/mL from both crystal violet assay and XTT assay, which are more than 8-fold higher than those of pure AgNWs and ZnFe2O4 NPs. This greatly enhanced biofilm inhibition activity is attributed to the ZnFe2O4 NPs-carrying membrane penetration by AgNWs and the subsequent interaction between Candida cells and ZnFe2O4 NPs. These results indicate that the ZnFe2O4@AgNWs hybrid nanostructures have great potential as a new type of novel antibiofilm agent.

Dual antifungal activity againstCandida albicansof copper metallic nanostructures and hierarchical copper oxide marigold‐like nanostructures grown in situ in the culture medium

“…Thakur et al . (2019) showed the randomly distributed AgNWs over surface partially penetrate inside Candida biofilms participating in the early cell death (Thakur et al . 2019).…”

“…The diameter of NWs has a strong influence on its texture (Karim, et al 2008). Thakur et al (2019) showed the randomly distributed AgNWs over surface partially penetrate inside Candida biofilms participating in the early cell death (Thakur et al 2019). Parameters of NPs and NWs such as size, shape and surface characteristics allow them to interact closely with the cell wall and cell membranes of the micro-organisms (Chatterjee et al 2014).…”

Current applications and prospects of nanoparticles for antifungal drug delivery

“…Thakur's study in 2019 indicated that the zinc ferrite nanoparticles and silver nanowires (ZnFe 2 O 4 @AgNWs) manifest both biofilm inhibition capacity for Candida albicans cells and potentially novel antifungal activities. They showed that AgNWs penetrated the biofilm matrix and transported ZnFe 2 O 4 NPs into it, so ZnFe 2 O 4 NPs could damage Candida cells by generating reactive oxygen species (ROS) (Thakur et al, 2019[ 112 ]). Szerencsés showed the same results by using citrate-coated AgNPs to inhibit the biofilm formation of Candida (Szerencsés et al, 2020[ 109 ]).…”

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Evaluation of nanomaterials to prevent oral Candidiasis in PMMA based denture wearing patients. A systematic analysis

“…These results specify that the Zinc Ferrite Nanoparticles hybrid nanostructures have great potential to be a material of choice to prevent candida infection in denture patients. 51…”