The synergistic effect of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside combined with Adriamycin on MCF-7 breast cancer cells

Abstract

ObjectiveBreast cancer has been reported to be a serious disease and a threat to women’s health. 2,3,5,4′-Tetrahydroxystilbene-2-O-β-d-glucoside (THSG) is a bioactive natural compound originating from Polygonum multiflorum Thunb., which has been shown to possess anti-inflammatory and antitumor properties. Adriamycin (ADM) is a chemotherapy agent used in tumor therapy that is limited by its side effects. However, little is known about the synergistic effect of THSG combined with ADM on breast cancer. This study seeks to investigate the effects of the combination of THSG plus ADM on MCF-7 breast cancer cells and to test the mechanisms involved.Materials and methodsMTT assay was detected to determine cell viability. Furthermore, cell apoptosis was tested by flow cytometry and TUNEL assay. In addition, protein expression was measured by Western blot analysis.ResultsThe individual treatment of THSG and ADM induced cell injury. Moreover, cotreatment further increased it, which the effect may be associated with the elevation of the apoptotic-related protein expression such as Bax/Bcl-2 and cleaved caspase-3/caspase-3. Lastly, our results also show the reduction of vascular endothelial growth factor/phosphatidylinositol 3-kinase/Akt protein expression in the individual or synergistic treatment.ConclusionTaken together, cotreatment of THSG and ADM may exert a synergistic reduction of cell injury via the inhibition of vascular endothelial growth factor/phosphatidylinositol 3-kinase/Akt pathway. Thus, THSG might possess potent anti-breast cancer effect with ADM.

2,3,5,4′-tetrahydroxystilbene-2-O-b-D-glucoside triggers the pluripotent-like possibility of dental pulp stem cells by activating the JAK2/STAT3 axis: Preliminary observations

“…It has been reported that THSG treatment significantly impedes cancer growth and invasion in vitro and in vivo . 16 However, in the present study, the MTS assay results indicated that THSG significantly enhanced the proliferation of DPSCs when the concentration was higher than 1 μM, with no cytotoxic effects at 48 and 96 h ( Fig. 1 A).…”

Anticancer Potential of Resveratrol, β-Lapachone and Their Analogues

“…In vivo assays with THSG, using azoxymethane-induced colorectal cancer in rats, induced a 50% reduction in total colonic aberrant crypt foci by the inhibition of NF-κB pathway activation [67]. In MCF-7 breast cancer cells exposed to adriamycin and THSG, the vascular endothelial growth factor/phosphatidylinositol 3-kinase/Akt pathway was inhibited, triggering apoptosis by modulating Bcl-2 and caspase-3 [68].…”

Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study

“…Stilbenes, mainly 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (TSG), possessed antioxidative, antitumor, anti-inflammatory, endothelial protective, neuroprotective, and liver-protective activities, etc. [10][11][12][13][14][15][16][17][18][19][20][21]. Anthraquinones were reported to possess many biological activities, including immunomodulating, anticancer, antimutation, antibacterial, anticancer, antioxidant, etc.…”