The swine histo compatibility system SLA: serological studies in the common Swiss and Danish swine breeds

Abstract

SummaryAlloantisera were produced in common Swiss and Danish swine breeds by immunization with leucocytes or skin‐grafts. Out of the 126 antisera, 66 were chosen for further study based on titrations employing lymphocytes from unrelated pigs typed with French SLA antisera (Vaiman, Chardon & Renard, 1979). The 66 selected antisera and the French reagents defining 26 SLA specificities were used to type lymphocytes from 595 unrelated pigs of the common Swiss and Danish breeds. The reaction‐patterns of the French, Swiss and Danish antisera were adequately correlated for the French SLA specificities Nos FJ 2, 3, 6, 7, 8, 9, 11, 14, 19, 20 and 24 and for the haplotypes FJ 15.1.18 and FJ 5.4. In addition, a cluster of correlated Danish and Swiss antisera characterized a new specificity, provisionally designated CPH 31. This specificity was frequent in the Danish Landrace pigs.Using the reagents identified in this report, the segregation of SLA markers was studied. Back‐cross families demonstrated segregation of 15 distinct SLA haplotypes of which 14 are common in French Landrace or Large White. Differences were found in haplotype frequencies in both the Swiss and the Danish Landrace and Large White breeds.

Genetic analysis of reproductive traits and antibody response in a PRRS outbreak herd1

“…Although associated with immune response activity, TAP1 has been shown to be expressed in the blastocyst stage in mice, indicating that this gene may also play a role in embryo preimplantation (Cooper et al, 1998). In general, associations between the MHC region/genes and reproductive processes have been extensively reported in the literature, with spontaneous abortion and infertility in humans (Jin et al, 1995), in reproductive success in mice (Warner, 1986;Liljander et al, 2006), and in reproductive traits in the pig, such as piglet mortality (Kristensen, 1985), ovulation rate (Rothschild et al, 1984), and fetus survival (Joyce et al, 2008), supporting the results that ELISA S/P ratio may be used as an indicator trait for reproductive traits.…”

Detection of a new non‐MHC alloantigenic system (SLD) in pigs

“…SLA antigens in the Czech pigs were detected by 14 groups of cytotoxic sera determining antigens known under the laboratory numbers Ll-Ll4 (Hruban et al 1977). SLA antigens in the Danish pigs were detected by 37 French and 28 Danish antisera detecting 23 different antigenic specificities (Kristensen et al, 1985).…”

A new case of blood cell chimerism in pigs

“…According to the standard procedures of progeny testing stations, No 241 was slaughtered at 90 kg ( 5 months of age) and further tests were excluded.SLA typing was performed on lymphocytes from the apparent chimeric pig (No 241), the parents and two littermates. The class I SLA antigens were determined with the panel of SLA antisera described by Kristensen et al (1985) using the micro-lymphocytotoxicity test. Four distinct SLA haplotypes were demonstrated on the lymphocytes from the chimeric pig, namely FJ 20-8.2.11, FJ 20-7.2, FJ 10.14 235…”

“…SLA typing was performed on lymphocytes from the apparent chimeric pig (No 241), the parents and two littermates. The class I SLA antigens were determined with the panel of SLA antisera described by Kristensen et al (1985) and CPH 8.31 (Table 2). In contrast the two littermates, which were identical, possessed only two haplotypes (CPH 8.31 and FJ 20-8.2.11).…”