The Surface Protein SdrF Mediates Staphylococcus epidermidis Adherence to Keratin

Trivedi, Sheetal; Uhlemann, Anne‐Catrin; Herman‐Bausier, Philippe; Sullivan, Sean B.; Sowash, Madeleine; Flores, Elizabeth Y; Khan, Sobia; Dufrêne, Yves F.; Lowy, Franklin D.

doi:10.1093/infdis/jix213

Cited by 19 publications

(16 citation statements)

References 30 publications

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“…Also involved in the adhesion of S. epidermidis to biotic surfaces are the proteins SdrF [71,72], SesC [73] and Embp [74], due to their affinity to, respectively, collagen and keratin, fibrinogen and fibronectin. In other CoNS additional adhesins with specificity to bind collagen were found, such as the SrdX in S. capitis [75], and the protein SrdI in S. saprophyticus that binds to both collagen [76] and fibronectin [77].…”

Section: Initial Adhesion To Abiotic Surfacesmentioning

confidence: 99%

Virulence Factors in Coagulase-Negative Staphylococci

et al. 2021

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Coagulase-negative staphylococci (CoNS) have emerged as major pathogens in healthcare-associated facilities, being S. epidermidis, S. haemolyticus and, more recently, S. lugdunensis, the most clinically relevant species. Despite being less virulent than the well-studied pathogen S. aureus, the number of CoNS strains sequenced is constantly increasing and, with that, the number of virulence factors identified in those strains. In this regard, biofilm formation is considered the most important. Besides virulence factors, the presence of several antibiotic-resistance genes identified in CoNS is worrisome and makes treatment very challenging. In this review, we analyzed the different aspects involved in CoNS virulence and their impact on health and food.

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Section: Initial Adhesion To Abiotic Surfacesmentioning

confidence: 99%

Virulence Factors in Coagulase-Negative Staphylococci

et al. 2021

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“…Adhesion of S. epidermidis cells in biofilms is mediated by polysaccharide intercellular adhesin, a homoglycan composed of β-1,6-linked 2-deoxy-2-amino-D-glucopyranosyl residues [ 29 ]. It has been reported that this could be influenced by enhanced expression of atle , aap , agr , ica [ 30 ], and SdrF genes [ 31 ]. It may be speculated that the irrigating protocols have an impact on the gene expression influencing adhesion.…”

Section: Discussionmentioning

confidence: 99%

Root canal irrigants influence the hydrophobicity and adherence of Staphylococcus epidermidis to root canal dentin: an in vitro study

et al. 2018

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ObjectivesTo determine the effect of root canal irrigants on the hydrophobicity and adherence of Staphylococcus epidermidis (S. epidermidis) to root canal dentin in vitro.Materials and MethodsRoot dentin blocks (n = 60) were randomly divided into 4 groups based on the irrigation regimen: group 1, saline; group 2, 5.25% sodium hypochlorite (NaOCl); group 3, 5.25% NaOCl followed by 17% ethylenediaminetetraacetic acid (EDTA); group 4, same as group 3 followed by 2% chlorhexidine (CHX). The hydrophobicity of S. epidermidis to root dentin was calculated by cell surface hydrophobicity while the adherence was observed by fluorescence microscopy, and bacteria were quantified using ImageJ software (National Institutes of Health). Statistical analysis of the data was done using Kruskal-Wallis test and Mann-Whitney U test (p = 0.05).ResultsThe hydrophobicity and adherence of S. epidermidis to dentin were significantly increased after irrigating with group 3 (NaOCl-EDTA) (p < 0.05), whereas in group 4 (NaOCl-EDTA-CHX) both hydrophobicity and adherence were significantly reduced (p < 0.05).ConclusionsThe adherence of S. epidermidis to dentin was influenced differently by root canal irrigants. Final irrigation with CHX reduces the bacterial adherence and may impact biofilm formation.

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“…In S. epidermidis, three Sdr proteins have been identified; SdrF, SdrG (Fbe) and SdrH. SdrF has been shown to mediate strong binding to keratins, keratinocytes and nasal epithelial cells [59]. In S. aureus, SdrD has been shown to mediate adhesion to keratinocytes through binding to desmoglein1, expressed in human epidermis [60].…”

Section: Adhesion To Human Keratinocytes and Bacterial Surface Proteimentioning

confidence: 99%

Identification of surface proteins in a clinical Staphylococcus haemolyticus isolate by bacterial surface shaving

et al. 2020

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Background: The skin commensal Staphylococcus haemolyticus is an emerging nosocomial pathogen. Despite its clinical relevance, published information about S. haemolyticus virulence factors is scarce. In this study, the adhesive and biofilm forming properties of ten clinical and ten commensal S. haemolyticus strains were examined using standard adhesion and biofilm assays. One of the clinical strains was used to identify expressed surface proteins using bacterial surface shaving. Protein abundance was examined by a comparative analysis between bacterial protein expression after human keratinocyte (HaCaT) colonization and growth in cell culture media supplemented with serum. Relative protein quantification was performed by labeling peptides with tandem mass tags (TMT) prior to Mass Spectrometry analysis. Surface proteins can be used as novel targets for antimicrobial treatment and in diagnostics. Results: Adherence to fibronectin, collagen and plastic was low in all tested strains, but with significantly higher adhesion to fibronectin (p = 0.041) and collagen (p = 0.001) in the commensal strains. There was a trend towards higher degree of biofilm formation in the clinical strains (p = 0.059). By using surface shaving, 325 proteins were detected, of which 65 were classified as surface proteins. Analyses showed that the abundance of nineteen (5.8%) proteins were significantly changed following HaCaT colonization. The bacterial Toll/interleukin-1 like (TIRs) domain containing protein (p = 0.04), the transglycosylase SceD (p = 0.01), and the bifunctional autolysin Atl (p = 0.04) showed a 1.4, 1.6-and 1.5-fold increased abundance. The staphylococcal secretory antigen (SsaA) (p = 0.04) was significantly downregulated (− 1.5 fold change) following HaCaT colonization. Among the 65 surface proteins the elastin binding protein (Ebps), LPXAG and LPXSG domain containing proteins and five LPXTG domain containing proteins were identified; three Sdr-like proteins, the extracellular matrix binding protein Embp and a SasH-like protein.

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The Surface Protein SdrF Mediates Staphylococcus epidermidis Adherence to Keratin

Abstract: These studies demonstrate that SdrF mediates adherence to human keratin and suggest that SdrF may facilitate S. epidermidis colonization of the skin.

Cited by 19 publications

References 30 publications

Virulence Factors in Coagulase-Negative Staphylococci

Virulence Factors in Coagulase-Negative Staphylococci

Root canal irrigants influence the hydrophobicity and adherence of Staphylococcus epidermidis to root canal dentin: an in vitro study

Identification of surface proteins in a clinical Staphylococcus haemolyticus isolate by bacterial surface shaving

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